1887

Abstract

SUMMARY: A rapid and efficient method of transforming by electroporation is described. A number of factors are important in determining the level of transformation obtained. Cells grown in the presence of glycine and isonicotinic acid hydrazide and harvested in early exponential growth phase were much easier to transform. The recovery medium on which transformants were isolated also had a significant effect on the number of transformants obtained because cells were osmotically or electrochemically sensitive following electroporation. Transformation efficiencies of up to 5 x 10 transformants per μg plasmid DNA with homologously derived DNA and 2 x 10 transformants per μg of DNA derived from were obtained.

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/content/journal/micro/10.1099/00221287-136-2-255
1990-02-01
2019-10-23
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http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-136-2-255
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