Summary: In standard liquid medium containing 5g mannitol l and 1g glutamic acid l, SU-47 cells accumulated 350 mg cyclic 1,2-β-glucans (g protein). The cyclic glucans were 36% glycerol-1-phosphatesubstituted and 64% were uncharged. In the same medium with 10 g mannitol l, repeating units of succinoglycan (1110 mg l) were found as extracellular carbohydrates, and only low amouns of the succinoglycan polymer (up to 300 mg l) were excreted. By raising the omotic pressure of the medium by the addition of NaCl or other ionic and non-ionic osmolytes, succinoglycan production could be stimulated: up to 2·4 g l at 0·2 m-NaCl was produced at the expense of the repeating units. Above 0·2 m-NaCl growth was slowed down, and succinoglycan excretion diminished. At 1 -NaCl growth stopped completely. In standard medium containing 0·6 -NaCl the amount of cellular cyclic 1,2-β-glucans was lowered to 150 mg (g protein) out of which the glycerol-1-phosphatesubstituted glucan fraction was reduced to 15%. Instead, high amounts of oliogosaccharides were synthesized as osmoprotectants, with trehalose as the major component [up to 200 mg (g protein)]. Glycogen synthesis was completely suppressed at this salt conenctration, while poly β-hydroxyutyric acid syntheis was unaffected.


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