1887

Abstract

The recovery of sp. S14 cells from energy- and nutrient-starvation was monitored after the addition of glucose minimal medium. Upshift experiments were done throughout a starvation period of 200 h to determine whether cells were more responsive to nutrient addition at the onset of starvation, or if the previously described programme of starvation-induced cellular reorganization had to be completed before cells could become committed to recovery following nutritional upshifts. The kinetics of macromolecular synthesis (RNA, protein and DNA), the rate of respiration and changes in median cell volume in response to nutritional upshifts at different times of starvation were examined. The relative rates of RNA and protein synthesis increased immediately upon addition of glucose minimal medium; the increase in protein synthesis was not dependent on a parallel increase in RNA synthesis, indicating that the starved cells may have an excess of protein synthesizing machinery, including stable RNA and functional ribosomes. The subsequent increase in the rate of DNA replication was initiated approximately 60 min before the first apparent cell division at approximately one doubling of the theoretical minimal cell volume (V). Two-dimensional gel electrophoresis was used to demonstrate the fate of starvation-specific proteins during the upshift, and also the synthesis of recovery-induced proteins that were not found in starving cells. Most starvation-inducible proteins were repressed immediately at the onset of the nutritional upshift, while 11 of the 21 recovery-induced proteins identified were expressed exclusively during the maturation phase and were subsequently repressed at the onset of regrowth. The possible role of such maturation-specific proteins in the rapid formation of a reproductive cell committed to growth and division is discussed.

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1990-11-01
2021-10-19
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References

  1. Albertson N. H., Jones G. W., Kjelleberg S. 1987; The detection of starvation specific antigens in two marine bacteria. Journal of General Microbiology 133:2225–2231
    [Google Scholar]
  2. Albertson N. H., Nyström T., Kjelleberg S. 1990a; Starvation-induced modulations in binding protein-dependent glucose transport by the marine Vibrio sp. S14. FEMS Microbiology Letters 70:205–210
    [Google Scholar]
  3. Albertson N. H., Nyström T., Kjelleberg S. 1990b; Exoprotease activity during starvation of two marine bacteria. Applied and Environmental Microbiology 56:218–223
    [Google Scholar]
  4. Albertson N. H., Nyström T., Kjelleberg S. 1990c; Functional mRNA half-lives in the marine Vibrio sp. S14 during starvation and recovery. Journal of General Microbiology 136:2195–2199
    [Google Scholar]
  5. Amy P. S., Pauling C., Morita R. Y. 1983a; Starvation-survival processes of a marine Vibrio . Applied and Environmental Microbiology 45:1041–10481
    [Google Scholar]
  6. Amy P. S., Pauling C., Morita R. Y. 1983b; Recovery from nutrient starvation by a marine Vibrio sp. Applied and Environmental Microbiology 45:1685–1690
    [Google Scholar]
  7. Boschwitz H., Halvorson H. O., Keynan A., Milner Y. 1985; Trypsin-like enzymes from dormant and germinated spores of Bacillus cereus T and their possible involvement in germination. Journal of Bacteriology 164:302–309
    [Google Scholar]
  8. Dawson M. P., Humphrey B. A., Marshall K. C. 1981; Adhesion: a tactic in the survival strategy of a marine vibrio during starvation. Current Microbiology 6:195–199
    [Google Scholar]
  9. Delieu T., Walker D. A. 1972; An improved cathode for the measurement of photosynthetic oxygen evolution by isolated chloroplasts. New Phytology 71:201–205
    [Google Scholar]
  10. Donachie W. D., Robinson A. C. 1987; Cell division: parameter values and the process. In Escherichia coli and Salmonella typhi- murium, Cellular and Molecular Biology 2 pp. 1578–1593 Neidhardt F. C. Edited by Washington, DC: American Society for Microbiology;
    [Google Scholar]
  11. Dow C. S., Whittenbury R., Carr N. G. 1983; The ‘shut down’ or ‘growth precursor’ cell - an adaptation for survival in a potentially hostile environment. Symposia of the Society for General Microbiology 34:187–247
    [Google Scholar]
  12. Foster S. J., Johnstone K. 1989; The trigger mechanism of bacterial spore germination. In Regulation of Prokaryotic Development pp. 89–107 Smith I., Slepecky R. A., Setlow P. Edited by Washington, DC: American Society for Microbiology;
    [Google Scholar]
  13. Gould G. W. 1970; Germination and the problem of dormancy. Journal of Applied Bacteriology 33:34–49
    [Google Scholar]
  14. Helmstetter C. E., Pierucci O., Weinberger M., Holmes M., Tang M. -S. 1979; Control of cell division in Escherichia coli . In The Bacteria 7 pp. 517–579 Ornston J. R., SokatchL L. N. Edited by New York: Academic Press;
    [Google Scholar]
  15. Jones K. L., Rhodes-Roberts M. E. 1981; The survival of marine bacteria under starvation conditions. Journal of Bacteriology 50:247–258
    [Google Scholar]
  16. Kjelleberg S., Hermansson M. 1984; Starvation-induced effects on bacterial surface characteristics. Applied and Environmental Microbiology 48:497–503
    [Google Scholar]
  17. Kjelleberg S., Humphrey B. A., Marshall K. C. 1982; Effects of interfaces on small, starved marine bacteria. Applied and Environmental Microbiology 43:1166–1172
    [Google Scholar]
  18. Kjelleberg S., Humphrey B. A., Marshall K. C. 1983; Initial phase of starvation and activity of bacteria at surfaces. Applied and Environmental Microbiology 46:978–984
    [Google Scholar]
  19. Kjelleberg S., Hermansson M., Mårdén P., Jones G. W. 1987; The transient phase between growth and nongrowth of heterotrophic bacteria, with emphasis on the marine environment. Annual Review of Microbiology 41:25–49
    [Google Scholar]
  20. Koch A. L. 1971; The adaptive response of Escherichia coli to a feast and famine existence. Advances in Microbial Physiology 6:147–217
    [Google Scholar]
  21. Koch A. L. 1979; Microbial growth at low concentrations of nutrients. In Strategies of Microbial Life in Extreme Environments, Dahlem Konferenzen pp. 261–279 Shilo M. Edited by Weinheim: Verlag Chemie;
    [Google Scholar]
  22. Kuznetsov S. I., Dubina A. G., Lapteva N. A. 1979; Biology of oligotrophic bacteria. Annual Review of Microbiology 33:377–387
    [Google Scholar]
  23. Maaløe O. 1979; Regulation of the protein-synthesizing machinery ribosomes, tRNA, factors and so on. In Biological Regulation and Development 1 Goldberger R. F. Edited by New York: Plenum Press;
    [Google Scholar]
  24. Malmcrona-Friberg K., Tunlid A., Mårdén P., Kjelleberg S., Odham G. 1986; Chemical changes in the cell envelope and poly-β-hydroxybutyrate during short term starvation of a marine bacterial isolate. Archives of Microbiology 144:340–345
    [Google Scholar]
  25. Mårdén P., Tunlid A., Malmcrona-Friberg K., Odham G., Kjelleberg S. 1985; Physiological and morphological changes during short term starvation of marine bacterial isolates. Archives of Microbiology 142:326–332
    [Google Scholar]
  26. Mårdén P., Nyström T., Kjelleberg S. 1987; Uptake of leucine by a marine Gram-negative heterotrophic bacteria isolated from marine waters. FEMS Microbiology Ecology 45:233–241
    [Google Scholar]
  27. Mårdén P., Hermansson M., Kjelleberg S. 1988; Incorporation of tritiated thymidine by marine bacterial isolates when undergoing a starvation survival response. Archives of Microbiology 149:427–432
    [Google Scholar]
  28. Mencher J. R., Blankenship L. C. 1971; Enhancement of Bacillus cereus spore lytic enzyme by heat labile non-dialysable factor in spore extracts. Biochimica et Biophysica Acta 230:646–648
    [Google Scholar]
  29. Morita R. Y. 1982; Starvation-survival of heterotrophs in the marine environment. Advances in Microbial Ecology 6:171–198
    [Google Scholar]
  30. Morita R. Y. 1985; Starvation and miniaturisation of heterotrophs, with special emphasis on maintenance of the starved viable state. In Bacteria in Their Natural Environments pp. 111–130 Fletcher M. M., Floodgate G. D. Edited by London: Academic Press;
    [Google Scholar]
  31. Novitsky J. A., Morita R. Y. 1976; Morphological characterization of small cells resulting from nutrient starvation of a psychrophilic marine vibrio. Applied and Environmental Microbiology 32:617–622
    [Google Scholar]
  32. Novitsky J. A., Morita R. Y. 1977; Survival of a psychrophilic marine vibrio under long-term nutrient starvation. Applied and Environmental Microbiology 33:635–641
    [Google Scholar]
  33. Nyström T., Kjelleberg S. 1987; The effect of cadmium on starved heterotrophic bacteria isolated from marine waters. FEMS Microbiology Ecology 45:143–151
    [Google Scholar]
  34. Nyström T., Kjelleberg S. 1989; Role of protein synthesis in the cell division and starvation induced resistance to autolysis of a marine Vibrio during the initial phase of starvation. Journal of General Microbiology 135:1599–1606
    [Google Scholar]
  35. Nyström T., Mårdén P., Kjelleberg S. 1986; Relative changes in the incorporation rates of leucine and methionine during starvation survival of two bacteria isolated from marine waters. FEMS Microbiology Ecology 38:285–292
    [Google Scholar]
  36. Nyström T., Albertson N., Kjelleberg S. 1988; Synthesis of membrane and periplasmic proteins during starvation of a marine Vibrio sp. Journal of General Microbiology 134:1645–1651
    [Google Scholar]
  37. Nyström T., Albertson N. H., Kjelleberg S. 1989; Physiological and molecular adaptation to non-growth by marine vibrios. Recent Advances in Microbial Ecology 5:80–84
    [Google Scholar]
  38. O’Farrell P. H. 1975; High resolution two-dimensional electrophoresis of proteins. Journal of Biological Chemistry 250:4007–4021
    [Google Scholar]
  39. Poindexter J. S. 1981a; Oligotrophy: fast and famine existence. Advances in Microbial Ecology 5:63–89
    [Google Scholar]
  40. Poindexter J. S. 1981b; The caulobacters: ubiquitous unusual bacteria. Microbiological Reviews 45:123–179
    [Google Scholar]
  41. Tempest D. W., Neijssel O. M. 1981 Basic Life Sciences 18:335–356
    [Google Scholar]
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