1887

Abstract

An extracellular endoglucanase (1,4--glucanohydrolase, EC 3.2.1.4) produced by D-14 (ATCC 48104) has been purified to homogeneity by ammonium sulphate precipitation and two consecutive ion-exchange chromatographic steps on DEAE-Sephadex A-50 columns. The enzyme was purified 13·8-fold and was homogeneous by analytical PAGE and SDS-PAGE. It has a high apparent , of about 100000. The pH and temperature optima for its activity were 4·8 and 65°C respectively. The of the purified enzyme for CMC (sodium salt) was 3 mg ml. The enzyme displayed low activity toward salicin and -nitrophenyl --glucoside. The activity was enhanced in the presence of Na, K and Ca but effectively inhibited by Hg, Fe, Mg, Cu and NH . Inhibition studies indicated that the enzyme may be a metalloprotein and/or that it requires metal lons for its optimum activity.

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1990-10-01
2024-04-26
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References

  1. Andrews P. 1964; Estimation of molecular weight of protein by Sephadex gel filtration. Biochemical Journal 91:222–233
    [Google Scholar]
  2. Au K.S., Chan H.Y. 1987; Purification and properties of the endo-l,4-β-glucanase from Bacillus subtilis. Journal of General Microbiology 133:2155–2162
    [Google Scholar]
  3. Davis B.J. 1964; Disc electrophoresis. II. Method and application to serum proteins. Annals of the New York Academy of Sciences 121:404–427
    [Google Scholar]
  4. Dubois M., Gilles K.A., Hamilton J.K., Rebers P.A., Smith F. 1956; Colorimetric method for determination of sugars and related substances. Analytical Chemistry 28:350–356
    [Google Scholar]
  5. Eriksen J., Goksoyr J. 1976; The effect of temperature on growth and cellulase (β-1,4-endoglucanase) production in the compost fungus ChaetomiumthermophUe var. dissitum. Archives of Microbiology 110:233–238
    [Google Scholar]
  6. Eriksson K.E., Pettersson B. 1975; Extracellular enzyme system utilized by the fungus Sporotrichumpulverulentum(Chrysosporiumlignorum) for the breakdown of cellulose. European Journal of Biochemistry 51:193–206
    [Google Scholar]
  7. Funaguma T., Hara A. 1988; Purification and properties of two β-glucosidases from PenicilliumhergueiBanier and Sartory. Agricultural and Biological Chemistry 52:749–755
    [Google Scholar]
  8. Laemmli U.K. 1970; Cleavage of structural proteins during assembly of the head of the bacteriophage T4. Nature; London: 227680–685
    [Google Scholar]
  9. Langsford M.L., Gilkes N.R., Wakarchuk W.W., Kilburn D.G., Miller R.C.Jr warren R.A.J. 1984; The cellulase system of Cellulomonasfimi. Journal of General Microbiology 130:1367–1376
    [Google Scholar]
  10. Lupo D., Stutzenberger F. 1988; Changes in endoglucanase patterns during growth of Thermomonosporacurvata on cellulose. Applied and Environmental Microbiology 54:588–589
    [Google Scholar]
  11. Mackenzie C.R., Bilous D. 1982; Location and kinetic properties of the cellulase system of Acetivibriocellulolyticus. Canadian Journal of Microbiology 28:1158–1164
    [Google Scholar]
  12. Mandels M. 1982; Cellulases. In Annual Reports on Fermentation Processes, 5: pp 35–78 Edited by Tsao G.T. New York: Academic Press;
    [Google Scholar]
  13. Miller G.L. 1959; Use of dinitrosalicylic reagent for determination of reducing sugar. Analytical Chemistry 31:426–428
    [Google Scholar]
  14. Moreira A.R., Phillips J.A., Humphrey A.E. 1981; Production of cellulases by Thermomonospora sp. Biotechnology and Bioengineering 23:1339–1347
    [Google Scholar]
  15. Ng T.K., Zeikus J.G. 1981; Purification and characterization of an endoglucanase from Clostridium thermocellum. Biochemical Journal 199:341–350
    [Google Scholar]
  16. Olutiola P.O. 1982; Characterization of cellulase from Humicolalanuginosa. Experientia 38:1332–1333
    [Google Scholar]
  17. Pugsley A.P., Schwartz M. 1985; Export and secretion of proteins by bacteria. FEMS Microbiology Reviews 32:3–38
    [Google Scholar]
  18. Ramasamy K., Verachtert H. 1980; Location of cellulase components in Pseudomonas sp.isolated from activated sludge. Journal of General Microbiology 117:181–191
    [Google Scholar]
  19. Romanelli R.A., Houston C.W., Barnett S.M. 1975; Studies on thermophilic fungi. Applied Microbiology 30:276–281
    [Google Scholar]
  20. Rosenberg S.L. 1975; Temperature and pH optima for 21 species of thermophilic and thermotolerant fungi. Canadian Journal of Microbiology 21:1535–1540
    [Google Scholar]
  21. Roy S.K., Raha S.K., Dey S.K., Chakrabarty S.L. 1988; Induction and catabolite repression of β-glucosidase synthesis in Myceliophthorathermophila D-14 (ATCC 48104). Applied and Environmental Microbiology 54:2152–2153
    [Google Scholar]
  22. Roy S.K., Raha S.K., Dey S.K., Chakrabarty S.L. 1989; Immobilization of β-glucosidase from MyceliophthorathermophilaD-14. Enzyme and Microbial Technology 11:431–435
    [Google Scholar]
  23. Roy S.K., Raha S.K., Dey S.K., Chakrabarty S.L. 1990; Effect of temperature on the production and location of cellulase complex in Myceliophthorathermophila D-14. Enzyme and Microbial Technology in the Press
    [Google Scholar]
  24. Sen S., Abraham T.K., Chakrabarty S.L. 1981; Cellulolytic activity of Myceliophthorathermophila D-14. Current Science 50:598–600
    [Google Scholar]
  25. Sen S., Abraham T.K., Chakrabarty S.L. 1982; Characteristics of the cellulase produced by Myceliophthorathermophila D-14. Canadian Journal of Microbiology 28:271–277
    [Google Scholar]
  26. Sen S., Abraham T.K., Chakrabarty S.L. 1983; Induction of cellulase in Myceliophthorathermophila D-14. Canadian Journal of Microbiology 29:1258–1260
    [Google Scholar]
  27. Shoemaker S.P., Brown R.D.Jr 1978; Characterization of endo- 1,4-β-D-glucanases purified from Trichodermaviride. Biochimica et Biophysica Acta 523:147–161
    [Google Scholar]
  28. Stewart B.J., Leatherwood J.M. 1976; Derepressed synthesis of cellulase by Cellulomonas. Journal of Bacteriology 128:609–615
    [Google Scholar]
  29. Tong C.C., Cole A.L., Shepherd M.F. 1980; Purification and properties of the cellulases from the thermophilic fungus Thermoascusaurantiacus. Biochemical Journal 191:83–94
    [Google Scholar]
  30. Wood T.M., Mccrae S.L. 1978; The cellulase of Trichodermakoningii. Biochemical Journal 171:61–72
    [Google Scholar]
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