SUMMARY: The phylogenetic relationships of and were examined using reverse transcriptase sequencing of 16S ribosomal RNA. The sequences were aligned with other actinomycete sequences and a phylogenetic tree was constructed. ATCC 39323 and ATCC 31947 formed distinct sublines within the family Their status as distinct genera was supported by the results of DNA: DNA pairing experiments. The inclusion of and and two unpublished spp. sequences in the rRNA analysis resulted in joining the family In previous studies, this strain had formed an isolated line of descent. It is proposed that the genera and be classified in the family Further work is needed to confirm the phylogeny of Chemotaxonomic analyses of lipids and cell walls from support these findings and confirm previous work.


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