1887

Abstract

A library of genomic DNA fragments produced by partial 3A digestion was established in K12 using the plasmid vector pAT153. The library was screened using immune syphilitic rabbit serum and six recombinant phenotypes expressing eight treponemal polypeptides were detected. With two exceptions, all the recombinant gene products were the same size as polypeptides detected on Western immunoblots of The genes encoding three novel gene products, with molecular masses in SDS-PAGE of 42, 17 and 15·5 kDa, which had not been cloned previously from were also identified. Monoclonal antibodies which reacted with four of the eight recombinant polypeptides were generated.

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1989-09-01
2021-07-23
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References

  1. Bailey M.J., Cockayne A., Penn C.W. 1987; Monoclonal antibodies directed against surface- associated polypeptides of Treponemapallidum define a biologically active antigen.. Journal of General Microbiology 133:1793–1803
    [Google Scholar]
  2. Beall J.A., Mitchell G.F. 1986; Identification of a particular antigen from a parasite cDNA library using antibodies affinity purified from selected portions of Western blots.. Journal of Immunological Methods 86:217–233
    [Google Scholar]
  3. Borenstein L.A., Radolf J.D., Fehniger T.E., Blanco D.R., Miller J.N., Lovett M.A. 1988; Immunization of rabbits with recombinant Treponema pallidum surface antigen 4D alters the course of experimental syphilis.. Journal of Immunology 140:2415–2421
    [Google Scholar]
  4. Brahamsha B., Greenberg E.P. 1988; Immuno- electron microscope analysis of Spirochaetaaurantiaperiplasmic flagella and molecular cloning of a major axial filament antigen.. Abstracts of the Annual Meeting of the American Society for Microbiology p. 193:
    [Google Scholar]
  5. Broome W.S., Gilbert W. 1978; Immunological screening method to detect specific translation products.. Proceedings of the National Academy of Sciences of the United States of America 75:2746–2749
    [Google Scholar]
  6. Coates S.R., Sheridan P.J., Hansen D.S., Laird W.J., Erlich H.A. 1986; Serospecificity of a cloned protease-resistant Treponema pallidum-specific antigen expressed in Escherichia coli.. Journal of Clinical Microbiology 133:1397–1407
    [Google Scholar]
  7. Cockayne A., Bailey M.J., Penn C.W. 1987; Analysis of sheath and core structures of the axial filament of Treponema pallidum.. Journal of General Microbiology 133:1397–1407
    [Google Scholar]
  8. Van Embden J.D., Van Der Donk H.J., Van Eijk R.V., Van Der Heide H.G., De Jong J.A., Van Olderen M.F., Osterhaus A.D., Schouls L.M. 1983; Molecular cloning and expression of Treponema pallidum DNA in Escherichia coli K-12.. Infection and Immunity 42:187–196
    [Google Scholar]
  9. Fehniger T.E., Walfield A.M., Cunningham T.M., Radolf J.D., Miller J.N., Lovett M.A. 1984; Purification and characterization of a cloned protease-resistant Treponema pallidumspecific antigen.. Infection and Immunity 46:598–607
    [Google Scholar]
  10. Hanff P.A., Fehniger T.E., Miller J.N., Lovett M.A. 1982; Humoral immune response in human syphilis to polypeptides of Treponema pallidum.. Journal of Immunology 129:1287–1291
    [Google Scholar]
  11. Hanff P.A., Miller J.N., Lovett M.A. 1983a; Molecular characterisation of common treponemal antigens.. Infection and Immunity 40:825–828
    [Google Scholar]
  12. Hanff P.A., Bishop N.H., Miller J.N., Lovett M.A. 1983b; Humoral immune response in experimental syphilis to polypeptides of Treponema pallidum.. Journal of Immunology 131:1973–1977
    [Google Scholar]
  13. Hansen E.B., Pedersen P.E., Schouls L.M., Severin E., Van Embden J.D.A. 1985; Genetic characterization and partial sequence determination of a Treponema pallidum operon expressing two immunogenic membrane proteins in Escherichia coli.. Journal of Bacteriology 162:1227–1237
    [Google Scholar]
  14. Hensel U., Jobst-Wellensiek H., Bhakdi S. 1985; Sodium dodecyl sulphate-polyacrylamide gel electrophoresis immunoblotting as a serological tool in the diagnosis of syphilitic infections.. Journal of Clinical Microbiology 21:82–87
    [Google Scholar]
  15. Hindersson P., Bangsdorf J. 1987; Cloning and expression of 4 Treponema pallidum antigens identified by monoclonal antibodies.. Abstracts of the Annual Meeting of the American Society for Microbiology D47 p. 79:
    [Google Scholar]
  16. Hindersson P., Cockayne A., Schouls L.M., Van Embden J.D.A. 1986; Immunochemical characterization and purification of Treponema pallidumantigen TpD expressed by Escherichia coli K-12.. Sexually Transmitted Diseases 13:237–244
    [Google Scholar]
  17. Hindersson P., Knudsen J.D., Axelsen N.H. 1987; Cloning and expression of Treponema pallidum common antigen (Tp-4) in Escherichia coli K12.. Journal of General Microbiology 133:587–596
    [Google Scholar]
  18. Hsu P.-L., Qin M., Norris S.J., Sell S. 1988; Isolation and characterization of recombinant Escherichia coli clones secreting a 24-kilodalton antigen of Treponema pallidum.. Infection and Immunity 56:1135–1143
    [Google Scholar]
  19. Ijsselmuiden O.E., Schouls L.M., Stolz E., Aelbers G.N.M., Agterberg C.M., Van Embden J.D.A. 1989; Sensitivity and specificity of an enzyme linked immunosorbent assay using the recombinant DNA-derived Treponema pallidum protein TmpA for serodiagnosis of syphilis and the potential use of TmpA for assessing the effect of antibiotic therapy.. Journal of Clinical Microbiology 27:152–157
    [Google Scholar]
  20. Jones S.A., Marchitto K.S., Miller J.N., Norgard M.A. 1984; Monoclonal antibody with haemagglutination, immobilization, and neutralization activities defines an immunodominant, 47,000 mol. wt., surface-exposed immunogen of Treponema pallidum (Nichols).. Journal of Experimental Medicine 160:1404–1420
    [Google Scholar]
  21. Laemmli U.K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4.. Nature; London: 227680–685
    [Google Scholar]
  22. Lukehart S.A., Baker-Zander S.A., Gubish E.R. 1982; Identification of Treponema pallidumantigens: comparison with a non-pathogenic tre- poneme.. Journal of Immunology 129:833–838
    [Google Scholar]
  23. Maniatis T., Fritsch E.F., Sambrook J. 1982 Molecular Cloning: A Laboratory Manual. Cold Spring Harbor NY:: Cold Spring Harbor Laboratory.;
    [Google Scholar]
  24. Norgard M.V., Chamberlain N.R., Swancutt M.A., Goldberg M.S. 1986; Cloning and expression of the major 47-kilodalton surface immunogen of Treponema pallidum in Escherichia coli.. Infection and Immunity 54:500–506
    [Google Scholar]
  25. Norgard M.V., Miller J.N. 1983; Cloning and expression of Treponema pallidum (Nichols) antigen genes in Escherichia coli.. Infection and Immunity 42:435–445
    [Google Scholar]
  26. Norris S.J., Alderete J.F., Axelsen N.H., Bailey M.J., Baker-Zander S.A., Baseman J.B., Bassford P.J., Baughn R.E., Cockayne A., Hanff P.A., Hindersson P., Larsen S.A., Lovett M.A., Lukehart S.A., Miller J.N., Moskophidis M.A., Muller F., Norgard M.V., Penn C.W., Stamm L.V., Van Embden J.D., Wicher K. 1987; Identity of Treponema pallidum subsp. pallidum polypeptides: correlation of sodium dodecyl sulphate-polyacrylamide gel electrophoresis results from different laboratories.. Electrophoresis 8:77–92
    [Google Scholar]
  27. O’Connor C.G., Ashman L.K. 1982; Application of nitrocellulose transfer techniques and alkaline phosphatase-conjugated anti-immunoglobulin for determination of the specificity of monoclonal antibodies to protein mixtures.. Journal of Immuno-logical Methods 54:267–271
    [Google Scholar]
  28. Pedersen N.S., Axelsen N.H., Jorgensen B.B., Petersen C.S. 1980; Antibodies in secondary syphilis against five of forty Reiter treponeme antigens.. Scandinavian Journal of Immunology 11:629–633
    [Google Scholar]
  29. Penn C.W. 1983; The use of the rabbit as a model for syphilis infection.. In Experimental Bacterial and Parasitic Infections pp. 145–151 Keusch G., Wadstrom T. Edited by Amsterdam: Elsevier Biomedical;
    [Google Scholar]
  30. Penn C.W., Cockayne A., Bailey M.J. 1985; The outer membrane of Treponema pallidum: biological significance and biochemical properties.. Journal of General Microbiology 131:2349–2357
    [Google Scholar]
  31. Peterson K.M., Baseman J.B., Alderete J.F. 1986; Isolation of a Treponema pallidum gene encoding immunodominant outer envelope protein P6, which reacts with sera from patients at different stages of syphilis.. Journal of Experimental Medicine 164:1160–1170
    [Google Scholar]
  32. Radolf J.D., Norgard M.V. 1988; Pathogen specificity of T. pallidum subsp. pallidum membrane proteins identified by phase partitioning with Triton X-114.. Infection and Immunity 56:1825–1828
    [Google Scholar]
  33. Sanchez P.J., Mccracken G.H. Jr Wendel G.D., Olsen K., Threlkeld N., Norgard M.V. 1989; Molecular analysis of the fetal IgM response to Treponema pallidum antigens: implications for improved serodiagnosis of congenital syphilis.. Journal of Infectious Diseases 159:508–517
    [Google Scholar]
  34. Stamm L.V., Folds J.D., Bassford P.J. Jr 1982; Expression of Treponema pallidum antigens in Escherichia coli K-12.. Infection and Immunity 36:1238–1241
    [Google Scholar]
  35. Strugnell R.A., Williams W.F., Drummond L.P., Pedersen J.S., Toh B.H., Faine S. 1986; Development of increased seroblot reactivity against a 45,000 dalton polypeptide of Treponema pallidum(Nichols) correlates with the establishment of chancre immunity in syphilitic rabbits.. Infection and Immunity 51:957–960
    [Google Scholar]
  36. Swancutt M.A., Twehous D.A., Norgard M.V. 1986; Monoclonal antibody selection and analysis of a recombinant DNA-derived surface immunogen of Treponema pallidum expressed in Escherichia coli.. Infection and Immunity 52:110–119
    [Google Scholar]
  37. Twigg A.J., Sherratt D.J. 1980; Trans- complementable copy-number mutants of plasmid ColEl.. Nature; London: 283216–218
    [Google Scholar]
  38. Walfield A.M., Hanff P.A., Lovett M.A. 1982; Expression of Treponema pallidum antigens in Escherichia coli.. Science 216:522–523
    [Google Scholar]
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