1887

Abstract

Mutants of the yeast , exhibiting decreased ability to utilize -glucose as the sole carbon source, were obtained by selecting for resistance to 2-deoxyglucose. Enzyme studies confirmed that these strains are defective in the hexose-phosphorylating enzymes and are unable to phosphorylate -glucose to -glucose 6-phosphate. The results confirmed the presence of two hexokinases, A and B, with ratios of -glucose to -fructose phosphorylation activity of 1·3/1·0 and 3·0/1·0, respectively, and a -glucose-specific glucokinase. The behaviour of a hexose-negative strain, able to ferment -xylose in the presence of -glucose, is described.

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/content/journal/micro/10.1099/00221287-135-7-2019
1989-07-01
2022-01-24
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