1887

Abstract

Mutants of the yeast , exhibiting decreased ability to utilize -glucose as the sole carbon source, were obtained by selecting for resistance to 2-deoxyglucose. Enzyme studies confirmed that these strains are defective in the hexose-phosphorylating enzymes and are unable to phosphorylate -glucose to -glucose 6-phosphate. The results confirmed the presence of two hexokinases, A and B, with ratios of -glucose to -fructose phosphorylation activity of 1·3/1·0 and 3·0/1·0, respectively, and a -glucose-specific glucokinase. The behaviour of a hexose-negative strain, able to ferment -xylose in the presence of -glucose, is described.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-135-7-2019
1989-07-01
2024-05-12
Loading full text...

Full text loading...

/deliver/fulltext/micro/135/7/mic-135-7-2019.html?itemId=/content/journal/micro/10.1099/00221287-135-7-2019&mimeType=html&fmt=ahah

References

  1. Barnard E. A. 1975; Hexokinases from yeast.. Methods in Enzymology 42:6–20
    [Google Scholar]
  2. Bicho P. A., Runnals P. L., Cunningham J. D., Lee H. 1988; Induction of xylose reductase and xylitol dehydrogenase activities in Pachysolen tanno- philus and Pichia stipitis on mixed sugars.. Applied and Environmental Microbiology 54:50–54
    [Google Scholar]
  3. Colowick S.P. 1973; The hexokinases.. In The Enzymes, 3rd edn. 9 pp. 1–48 Boyer P. D. Edited by New York: Academic Press;
    [Google Scholar]
  4. Ditzelmuller G., Kubicek C. P., Wohrer W., Rohr M. 1984a; Xylose metabolism in Pachysolen tannophilus: purification and properties of xylose reductase.. Canadian Journal of Microbiology 30:1330–1336
    [Google Scholar]
  5. Ditzelmuller G., Kubicek C. P., Wohrer W., Rohr M. 1984b; Xylitol dehydrogenase from Pachysolen tannophilus.. FEMS Microbiology Letters 25:195–198
    [Google Scholar]
  6. Entian K.-D., Mecke D. 1982; Genetic evidence for a role of hexokinase isoenzyme PII in carbon catabolite repression in Saccharomyces cerevisiae.. Journal of Biological Chemistry 257:870–874
    [Google Scholar]
  7. Fernández R., Herrero P., Moreno F. 1985; Inhibition and inactivation of glucose-phosphorylat- ing enzymes from Saccharomyces cerevisiae by d-xylose.. Journal of General Microbiology 131:2705–2709
    [Google Scholar]
  8. Frohlich K.-U., Entian K.-D., Mecke D. 1984; Cloning and restriction analysis of the hexokinase PII gene of the yeast Saccharomyces cerevisiae.. Molecular and General Genetics 194:144–148
    [Google Scholar]
  9. Gancedo J., Clifton D., Fraenkel D. G. 1977; Yeast hexokinase mutants.. Journal of Biological Chemistry 252:4443–4444
    [Google Scholar]
  10. James A.P., Zahab D. M. 1982; A genetic system for Pachysolen tannophilus, a pentose-fermenting yeast.. Journal of General Microbiology 128:2297–2301
    [Google Scholar]
  11. James A. P., Zahab D. M. 1983; The construction and genetic analysis of polyploids and aneuploids of the pentose-fermenting yeast, Pachysolen tannophilus.. Journal of General Microbiology 129:2489–2494
    [Google Scholar]
  12. Lobo Z., Maitra P. K. 1977a; Resistance to 2-deoxyglucose in yeast: a direct selection of mutants lacking glucose-phosphorylating enzymes.. Molecular and General Genetics 152:297–300
    [Google Scholar]
  13. Lobo Z., Maitra P. K. 1977b; Genetics of yeast hexokinase.. Genetics 86:727–744
    [Google Scholar]
  14. Maitra P. K. 1970; A glucokinase from Saccharomyces cerevisiae.. Journal of Biological Chemistry 245:2423–2431
    [Google Scholar]
  15. Maleszka L. G., James A. P., Ruiten H., Schneider H. 1983; Xylitol dehydrogenase mutants of Pachysolen tannophilus and the role of xylitol in d-xylose catabolism.. FEMS Microbiology Letters 17:227–229
    [Google Scholar]
  16. Morimoto S., Matsuo M., Azuma K., Sinskey A.J. 1986; Purification and properties of d-xylu- lose reductase from Pachysolen tannophilus.. Journal of Fermentation Technology 64:219–225
    [Google Scholar]
  17. Morimoto S., Takahiko T., Azuma K., Oshima T., Sinskey A.J. 1987; Purification and properties of aldose reductase from Pachysolen tannophilus.. Journal of Fermentation Technology 65:17–21
    [Google Scholar]
  18. Ramel A. H., Rustrum Y. M., Jones J. G., Barnard E. A. 1971; Yeast hexokinase. IV. Multiple forms of hexokinase in the yeast cell.. Biochemistry 10:3499–3508
    [Google Scholar]
  19. Slininger P. J., Bolen P. L., Kurthman C. P. 1987; Pachysolen tannophilus: properties and process considerations for ethanol production from d-xylose.. Enzyme and Microbial Technology 9:5–15
    [Google Scholar]
  20. Wedlock D. N., Thornton R. J. 1989; A hexokinase associated with catabolite repression in Pachysolen tannophilus.. Journal of General Microbiology 135:2013–2028
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-135-7-2019
Loading
/content/journal/micro/10.1099/00221287-135-7-2019
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error