1887

Abstract

The genes for () urease were cloned and the protein was overexpressed (up to 18% of total protein consisted of this enzyme) in several hosts. The small size of the DNA encoding urease (3·5 kb), the restriction map, and the regulation of enzyme expression directed by the recombinant plasmid are distinct from other cloned ureases. Nickel concentration did not affect urease gene expression, as demonstrated by the high levels of apoenzyme measured in cells grown in nickel-free media. However, nickel was required for urease activity. The overproducing recombinant strain was used for immunogold electron microscopic localization studies to demonstrate that urease is a cytoplasmic enzyme.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-135-6-1769
1989-06-01
2022-01-26
Loading full text...

Full text loading...

/deliver/fulltext/micro/135/6/mic-135-6-1769.html?itemId=/content/journal/micro/10.1099/00221287-135-6-1769&mimeType=html&fmt=ahah

References

  1. Armbruster B.L., Carlemalm E., Chiovetti R., Garavito R.M., Hobot J.A., Kellenberger E., Villiger W. 1982; Specimen preparation for electron microscopy using low temperature embed-ding resins. Journal de Microscopie 126:77–85
    [Google Scholar]
  2. Bast E. 1988; Nickel requirement for the formation of active urease in purple sulphur bacteria (Chromatiaceae).. Archives of Microbiology 150:6–10
    [Google Scholar]
  3. Bendayan M. 1984; Protein A-gold electron microscopic immunochemistry: methods, applications, and limitations.. Journal of Electron Microscopy Technique 1:243–270
    [Google Scholar]
  4. Blake M.S., Johnston K.H., Russell-Jones G.L., Gotschlich E.C. 1984; A rapid, sensitive method for detection of alkaline phosphatase- conjugated anti-antibody on western blots. Analytical Biochemistry 136:175–179
    [Google Scholar]
  5. Collins C.M., Falkow S. 1988; Genetic analysis of an Escherichia coli urease locus: evidence of DNA rearrangement. Journal of Bacteriology 170:1041–1045
    [Google Scholar]
  6. Doyle C.M., Arp D.J. 1988; Nickel affects expression of the nickel-containing hydrogenase of Alcaligenes latus.. Journal of Bacteriology 170:3891–3896
    [Google Scholar]
  7. Dretzen G., Bellard P., Sassone-Corsi P., Chambon P. 1981; A reliable method for recovery of DNA fragments from agarose and acrylamide gels. Analytical Biochemistry 112:295–298
    [Google Scholar]
  8. Friedrich B., Magasanik B. 1977; Urease of Klebsiella aerogenes: control of its synthesis by glutamine synthetase. Journal of Bacteriology 131:446–452
    [Google Scholar]
  9. Gerlach G.-F., Clegg S., Nichols W.A. 1988; Characterization of the genes encoding urease activity of Klebsiella pneumoniae.. FEMS Microbiology Letters 50:131–135
    [Google Scholar]
  10. Hanahan D. 1983; Studies on transformation of Escherichia coli with plasmids. Journal of Molecular Biology 166:557–580
    [Google Scholar]
  11. Hausinger R.P. 1987; Nickel utilization by microorganisms. Microbiological Reviews 51:22–42
    [Google Scholar]
  12. Herrera A., Elhai J., Hohn B., Wolk C.P. 1984; Infrequent cleavage of cloned Anabaena variabilis DNA by restriction endonucleases from A. variabilis.. Journal of Bacteriology 160:781–785
    [Google Scholar]
  13. Jones B.D., Mobley H.L.T. 1988; Proteus mirabilis urease: genetic organization, regulation, and expression of structural genes. Journal of Bacteriology 170:3342–3349
    [Google Scholar]
  14. Kado C.I., Liu S.-T. 1981; Rapid procedure for detection and isolation of large and small plasmids. Journal of Bacteriology 145:1365–1373
    [Google Scholar]
  15. Kellenberger E., Durrenberger M., Villiger W., Carlemalm E., Wurtz M. 1987; The efficiency of immunolabel on Lowicryl sections compared to theoretical predictions. Journal of Histochemistry and Cytochemistry 35:959–969
    [Google Scholar]
  16. Kim S.-D., Spizizen J. 1985; Molecular cloning and expression of Bacillus pasteurii urease gene in Escherichia coli.. Korean Journal of Applied Microbiology & Bioengineering 13:297–302
    [Google Scholar]
  17. Laemmli U.K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, London 227:680–685
    [Google Scholar]
  18. Mackerras A.H., Smith G.D. 1986; Urease activity of the cyanobacterium Anabaena cylindrica.. Journal of General Microbiology 132:2749–2752
    [Google Scholar]
  19. Maclachlan P.R., Sanderson K.E. 1985; Transformation of Salmonella typhimurium with plasmid DNA: differences between rough and smooth strains. Journal of Bacteriology 161:442–445
    [Google Scholar]
  20. Magasanik B. 1982; Genetic control of nitrogen assimilation in bacteria. Annual Review of Genetics 16:135–168
    [Google Scholar]
  21. Maniatis T., Fritsch E.F., Sambrook J. 1982 Molecular Cloning: a Laboratory Manual Cold Spring Harbor, NY: Cold Spring Harbor Laboratory;
    [Google Scholar]
  22. Mckinney M.M., Parkinson A. 1987; A simple, non-chromatographic procedure to purify immuno-globulins from serum and ascites fluid. Journal of Immunological Methods 96:271–278
    [Google Scholar]
  23. Mclean R.J.C., Cheng K.-J., Gould W.D., Costerton J.W. 1985; Cytochemical localization of urease in a rumen Staphylococcus sp. by electron microscopy. Applied and Environmental Microbiology 49:253–255
    [Google Scholar]
  24. Mclean R.J.C., Cheng K.-J., Gould W.D., Nickel J.C., Costerton J.W. 1986; Histoche- mical and biochemical urease localization in the periplasm and outer membrane of two Proteus mirabilis strains. Canadian Journal of Microbiology 32:772–778
    [Google Scholar]
  25. Messing J., Crea R., Seeburg P.H. 1981; A system for shotgun DNA sequencing. Nucleic Acids Research 9:309–321
    [Google Scholar]
  26. Miller J.H. 1972 Experiments in Molecular Genetics Cold Spring Harbor, NY: Cold Spring Harbor Laboratory;
    [Google Scholar]
  27. Mobley H.L.T., Jones B.D., Jerse A.E. 1986; Cloning of urease gene sequences from Providencia stuartii.. Infection and Immunity 54:161–169
    [Google Scholar]
  28. Mulrooney S.B., Lynch M.J., Mobley H.L.T., Hausinger R.P. 1988; Purification, characteriza-tion, and genetic organization of recombinant Providencia stuartii urease expressed by Escherichia coli.. Journal of Bacteriology 170:2202–2207
    [Google Scholar]
  29. Neidhardt F.C., Bloch P.L., Smith D.F. 1974; Culture media for enterobacteria. Journal of Bacteriology 119:736–747
    [Google Scholar]
  30. Ørskov I. 1974; Genus VI. Klebsiella Trevisan 1885. In Bergey’s Manual of Determinative Bacteriology, 8th edn. pp 321–324 Buchanan R. E., Gibbons N. E. Edited by Baltimore: Williams & Wilkins;
    [Google Scholar]
  31. Rees T.A.V., Bekheet I.A. 1982; The role of nickel in urea assimilation by algae. Planta 156:385–387
    [Google Scholar]
  32. Smith C.J., Hespell R.B., Bryant M.P. 1980; Ammonia assimilation and glutamate formation in the anaerobe Selenomonas ruminantium.. Journal of Bacteriology 141:593–602
    [Google Scholar]
  33. Soberon X., Covarrubias L., Bolivar F. 1980; Construction and characterization of new cloning vehicles, IV. Deletion derivatives of pBR322 and pBR325. Gene 9:287–305
    [Google Scholar]
  34. Stults L.W., Sray W.A., Maier R.J. 1986; Regulation of hydrogenase biosynthesis by nickel in Bradyrhizobium japonicum.. Archives of Microbiology 146:280–283
    [Google Scholar]
  35. Todd M.J., Hausinger R.P. 1987; Purification and characterization of the nickel-containing multi-component urease from Klebsiella aerogenes.. Journal of Biological Chemistry 262:5963–5967
    [Google Scholar]
  36. Vieira J., Messing J. 1982; The pUC plasmids, an M13mp7-derived system for insertion mutagenesis and sequencing with universal primers. Gene 19:259–268
    [Google Scholar]
  37. Walz S.E., Wray S.K., Hull S.I., Hull R.A. 1988; Multiple proteins encoded within the urease gene complex of Proteus mirabilis.. Journal of Bacteriology 170:1027–1033
    [Google Scholar]
  38. Winkler R.G., Polacco J.C., Eskew D.L., Welch R.M. 1983; Nickel is not required for apourease synthesis in soybean seeds. Plant Physiology 72:262–263
    [Google Scholar]
  39. Yanisch-Perron C., Vieira , Messing J. 1985; Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mpl8 and pUC19 vectors. Gene 33:103–119
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-135-6-1769
Loading
/content/journal/micro/10.1099/00221287-135-6-1769
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error