The expression of the fusion gene was partially repressed in the presence of an excess of glucose. Expression was restored either by the mutation () or by addition of decoyinine, an inhibitor of GMP synthetase, to the medium. By constructing a fusion with a smaller fragment of the gene, we observed a β-galactosidase profile in which expression was completely repressed by an excess of glucose. This expression was restored by the addition of decoyinine. These results indicate that the expression of the gene is regulated by at least two different mechanisms, one sensitive to glucose, the other not. Furthermore, the glucose-sensitive regulation was shown to reside at the transcriptional level. It is likely that the reduced expression of the gene in the presence of glucose at an early stage of sporulation causes the repression of sporulation.


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