1887

Abstract

A 26 bp DNA probe has been constructed with minimal degeneracy to the protein sequence for enterotoxin. The probe has been hybridized against a 6–10 kb chromosomal bank from 8239, prepared as a dIII partial digest in pHG165. From this survey a clone has been identified containing a 6·8 kb DNA insert with strong hybridization to the probe. Direct plasmid sequencing has identified a translational reading frame within this clone which correlates with the known protein sequence for the type A enterotoxin. DNA sequences 5′ to this open reading frame and containing the putative transcriptional control regions show areas of significant homology with regions upstream from the ATG codon of the tetanus toxin gene.

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/content/journal/micro/10.1099/00221287-135-4-903
1989-04-01
2022-01-18
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References

  1. Bartlett J.A., Gaillard R.K. JR Joklik W.K. 1986; Sequencing of supercoiled plasmid DNA. BioTechniques 4:208–210
    [Google Scholar]
  2. Bryan F.L. 1969; What the sanitarian should know about Clostridium perfringens food-borne illness. Journal of Milk Food Technology 32:381–389
    [Google Scholar]
  3. Cannon R.E., Tatti K.M., Wu G.J. 1985; A rapid, small-scale procedure for the preparation of plasmid DNA for screening mutants by the dide-oxynucleotide chain-terminator sequencing method. BioTechniques 3:93–96
    [Google Scholar]
  4. Duncan C.L. 1970; Clostridium perfringens food poisoning. Journal of Milk Food Technology 33:35–41
    [Google Scholar]
  5. Eisel U., Jarausch W., Goretzki K., Henschen A., Engels J., Weller U., Hudel M., Habermann E., Niemann H. 1986; Tetanus toxin: primary structure, expression in E. coli and homology with botulinum toxins. EMBO Journal 5:2495–2502
    [Google Scholar]
  6. Frieben W.R., Duncan C.L. 1973; Homology between enterotoxin protein and spore structural protein in Clostridium perfringens type A. European Journal of Biochemistry 39:393–401
    [Google Scholar]
  7. Genigeorgis C. 1975; Public health importance of Clostridium perfringens . Journal of the American Veterinary Medical Association 167:821–827
    [Google Scholar]
  8. Goldner S.B., Solberg M., Jones S., Post L.S. 1986; Enterotoxin synthesis by nonsporulating cultures of Clostridium perfringens . Applied and Environmental Microbiology 52:407–412
    [Google Scholar]
  9. Granum P.E. 1985; The amino-acid sequence of the enterotoxin from Clostridium perfringens type A. FEBS Letters 182(2):479–484
    [Google Scholar]
  10. Granum P.E., Telle V., Olsvik O., Stavn A. 1984; Enterotoxin formation by Clostridium perfringens during sporulation and vegetative growth. International Journal of Food Microbiology 1:43–49
    [Google Scholar]
  11. Guerry P., Leblanc D.J., Falkow S. 1973; General method for the isolation of plasmid deoxyribonucleic acid. Journal of Bacteriology 116:1064–1066
    [Google Scholar]
  12. Hatheway C.L., Whaley D.N., Dowell V.R. JR 1980; Epidemiological aspects of Clostridium perfringens foodbome illness. Food Technology 34:77–79 & 90
    [Google Scholar]
  13. Heath L.S., Sloan G.L., Heath H.E. 1986; A simple and generally applicable procedure for releasing DNA from bacterial cells. Applied and Environmental Microbiology 51:1138–1140
    [Google Scholar]
  14. Maniatis T., Fritsch E.F., Sambrook J. 1982 Molecular Cloning: a Laboratory Manual. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory;
    [Google Scholar]
  15. Marusyk R., Sergeant A. 1980; A simple method for dialysis of small-volume samples. Analytical Biochemistry 105:403–404
    [Google Scholar]
  16. Okita T.W., Rodriguez R.L., Preiss J. 1981; Cloning of the glycogen biosynthetic enzyme structural genes of Escherichia coli. Biosynthesis of bacterial glycogen. Journal of Biological Chemistry 256:6944–6952
    [Google Scholar]
  17. Ryu S., Labbe R. 1989; Analysis of spore coat proteins of Clostridium perfringens type A. In Tenth International Spores Conference -1988. Slepecky R., Smith I. Edited by Washington, DC: American Society for Microbiology; (in the Press)
    [Google Scholar]
  18. Shine J., Dalgarno C.L. 1975; Determination of cistron specificity in bacterial ribosomes. Nature, London 254:34–38
    [Google Scholar]
  19. Sloma A., Gross M. 1983; Molecular cloning and nucleotide sequence of the type 1 β-lactamase gene from Bacillus cereus . Nucleic Acids Research 11:4997–5004
    [Google Scholar]
  20. Smith W.P., Mcdonel J.L. 1980; Clostridium perfringens type A: in vitro system for sporulation and enterotoxin synthesis. Journal of Bacteriology 144:306–311
    [Google Scholar]
  21. Stark R.L., Duncan C.L. 1971; Biological characteristics of Clostridium perfringens type A enterotoxin. Infection and Immunity 4:89–96
    [Google Scholar]
  22. Stewart G.S.A.B., Lubinsky-Mink S., Jackson C.G., Cassel A., Kuhn J. 1986; pHG165: a pBR322 copy number derivative of pUC8 for cloning and expression. Plsamid 15:172–181
    [Google Scholar]
  23. Williams S.A., Slatko B.E., Moran L.S., Desimone S.M. 1986; Sequencing in the fast lane: a rapid protocol for [α-35S] dATP dideoxy DNA sequencing. BioTechniques 4:138–147
    [Google Scholar]
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