1887

Abstract

Exfoliated vaginal epithelial cells with attached bacteria, termed ‘clue cells’, which were procured from a patient with non-specific vaginitis, were stained with ruthenium red and examined by transmission electron microscopy. The attached bacteria appeared to adhere by means of an outer fibrillar coat. An epithelial tissue culture cell line (McCoy) and human red blood cells to which strains of attached were similarly examined. The adherence of to the epithelial cell line appeared to be mediated by an outer fibrillar coat while adherence to red cells appeared to be mediated by fimbriae. Transmission electron microscopy was performed on the strains used. Thin sections of tissue-culture-adherent strains revealed a dense outer fibrillar coat whereas the surface of the haemagglutinating strains showed fine fimbriae. Negative staining of haemagglutinating strains demonstrated fimbriae on a minority of organisms.

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/content/journal/micro/10.1099/00221287-135-3-475
1989-03-01
2022-01-23
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References

  1. Amsel R., Totten P.A., Spiegel C.A., Chen K.C.S., Eschenbach D., Holmes K.K. 1983; Non-specific vaginitis. American Journal of Medicine 74:14–22
    [Google Scholar]
  2. Boustouller Y.L., Johnson A.P., Taylor-Robinson D. 1987; Pili on Gardnerella vaginalis studied by electron microscopy. Journal of Medical Microbiology 23:327–329
    [Google Scholar]
  3. Crisswell B.S., Stenback W.A., Black S.H., Gardner H.L. 1972; Fine structure of Haemophilus vaginalis . Journal of Bacteriology 109:930–932
    [Google Scholar]
  4. Easmon C.S.P., Ison C.A. 1986; Gardnerella vaginalis and bacterial vaginosis. In Medical Micro-biology 4 pp 53–76 Easmon C. S. F., Jeljaszewicz J. Edited by London: Academic Press;
    [Google Scholar]
  5. Greenwood J.R. 1983; Current taxonomic status of Gardnerella vaginalis . Scandinavian Journal of Infectious Diseases (Supplement) 40:11–14
    [Google Scholar]
  6. Ison C.A., Easmon C.S.F. 1985; Studies on the mechanism of adhesion of Gardnerella vaginalis to human erythrocytes. Scandinavian Journal of Nephrology (Supplement) 86:190–193
    [Google Scholar]
  7. Johnson A.P., Davies H.A. 1984; Demonstration by electron microscopy of pili of Gardnerella vaginalis . British Journal of Venereal Diseases 60:196–197
    [Google Scholar]
  8. Knutton S., Williams P.H., Lloyd D.R., Candy D.C.A., Mcneish A.S. 1984; Ultrastructural study of adherence to and penetration of cultured cells by two invasive Escherichia coli strains isolated from infants with enteritis. Infection and Immunity 44:599–608
    [Google Scholar]
  9. Mårdh P.-A., Weström L. 1976; Adherence of bacteria to vaginal epithelial cells. Infection and Immunity 13:661–666
    [Google Scholar]
  10. Peeters M., Piot P. 1985; Adhesion of Gardnerella vaginalis to vaginal epithelial cells. Variables affecting adhesion and inhibition by metronidazole. Genitourinary Medicine 65:391–395
    [Google Scholar]
  11. Reyn A., Birch-Anderson A., Lapage S.P. 1966; An electron microscope study of thin sections of Haemophilus vaginalis (Gardner & Dukes) and some possibly related species. Canadian Journal of Microbiology 12:1125–1136
    [Google Scholar]
  12. Scott T.G., Smyth C.J. 1987; Haemagglutination and tissue culture adhesion of Gardnerella vaginalis . Journal of General Microbiology 133:1999–2005
    [Google Scholar]
  13. Scott T.G., Smyth C.J., Keane C.T. 1987; In vitro adhesiveness and biotype of Gardnerella vaginalis strains in relation to the occurrence of clue cells in vaginal discharges. Genitourinary Medicine 63:47–53
    [Google Scholar]
  14. Sobel J.D., Schneider J., Kaye D., Levison M. 1981; Adherence of bacteria to vaginal epithelial cells at various stages in the menstrual cycle. Infection and Immunity 32:194–197
    [Google Scholar]
  15. Taylor E., Phillips I. 1983; The identification of Gardnerella vaginalis . Journal of Medical Microbiology 16:83–92
    [Google Scholar]
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