1887

Abstract

A sp. isolated from compost degraded the hemicellulose fraction of straw efficiently but apparently not native cellulose. Ball-milled straw induced endoglucanase, -glucosidase, -xylanase and -xylosidase. Carboxymethylcellulose, cellotetraose and cellotriose induced cellulolytic enzymes specifically whereas cellobiose acted as inducer for -glucosidase only. Cellotriose and cellotetraose induced -glucosidase, but only partially induced endoglucanase. Hemicellulose (in the form of xylan) and xylobiose induced only -xylanase and -xylosidase. Kraft lignin and syringic acid induced -xylanase and endoglucanase but not the other enzymes. 3,4-Dimethoxycinnamic acid slightly induced -xylanase whereas 3,5-dimethoxy-4-hydroxycinnamic acid specifically induced endoglucanase. Neither veratric acid nor vanillic and ferulic acids induced any of the cellulolytic or hemicellulolytic enzymes. Enzyme production was subject to a form of carbon catabolite repression. Endoglucanase and -xylanase were excreted into the culture medium. Four protein components, one acidic (pI 5·2) and three basic (pI 8·15, 8·45 and 8·65) exhibited -xylanase activity. Two acidic components (pI 3·55 and 3·75) displayed endoglucanase activity.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-135-2-285
1989-02-01
2021-10-20
Loading full text...

Full text loading...

/deliver/fulltext/micro/135/2/mic-135-2-285.html?itemId=/content/journal/micro/10.1099/00221287-135-2-285&mimeType=html&fmt=ahah

References

  1. Ball A.S., Mccarthy A.J. 1988; Saccharification of straw by actinomycete enzymes. Journal of General Microbiology 134:2139–2147
    [Google Scholar]
  2. Bernier R. JR Desrochers M., Jurasek L., Paice M.G. 1983; Isolation and characterization of a xylanase from Bacillus subtilis . Applied and Environmental Microbiology 46:511–514
    [Google Scholar]
  3. Biely P., Vrsanska A., Kratky Z. 1980; Xylan degrading enzymes of the yeast Cryptococcus albidus . European Journal of Biochemistry 108:313–321
    [Google Scholar]
  4. Biely P., Mislovicova D., Toman R. 1985a; Soluble chromogenic substrates for the assay of endo-1,4-β-xylanases and endo-1,4-β-glucanases. Analytical Biochemistry 144:142–146
    [Google Scholar]
  5. Biely P., Markovic O., Mislovicova D. 1985b; Sensitive detection of endo-1,4-β-glucanases and endo-1,4-β-xylanases in gels. Analytical Biochemistry 144:147–154
    [Google Scholar]
  6. De Stevens. 1957; Preparation and properties of cellulose, hemicellulose and cellobiose. Methods in Enzymology 3:31–35
    [Google Scholar]
  7. Dubois M., Gilles K.A., Hamilton J.K., Rebers P.A., Smith F. 1956; Colorimetric methods for the determination of sugars and related substances. Analytical Chemistry 28:350–356
    [Google Scholar]
  8. Ferchak J.D., Pye E.K. 1983; Effect of glucose and other sugars on the /1-1,4-glucosidase of Ther-monospora fusca . Biotechnology and Bioengineering 25:2855–2864
    [Google Scholar]
  9. Flaigl W., Bentelspaecher H., Riets E. 1975; Chemical composition and physical properties of humic substances. In Soil Components 1 Organic Components,pp. 1–211 Gieseking J.E. Edited by Berlin: Springer-Verlag;
    [Google Scholar]
  10. Godden B., Penninckx M. 1984; Identification and evolution of the cellulolytic microflora present during composting of cattle manure: on the role of Actinomyces sp. Annales de microbiologie 135B:69–78
    [Google Scholar]
  11. Goodfellow M., Williams S.T. 1983; Ecology ofactinomycetes. Annual Review of Microbiology 126:189–216
    [Google Scholar]
  12. Halliwell G., Lovelady J. 1981; Utilization of carboxymethylcellulose and enzyme synthesis by Trichoderma koningii . Journal of General Microbiology 126:211–217
    [Google Scholar]
  13. Hopwood D.A., Bibb M.J., Chater K.F., Kieser T., Bruton C.J., Kieser H.M., Lydiate D.J., Smith C.P., Ward J.M., Schrempf H. 1985 Genetic Manipulation of Streptomyces pp. 3–5 Norich: John Innes Foundation;
    [Google Scholar]
  14. Kluepfel D., Ishaque M. 1982; Xylan induced cellulolytic enzymes in Streptomyces flavogriseus . Development Industrial Microbiology 23:389–396
    [Google Scholar]
  15. Kluepfel D., Shareck F., Mondou F., Moro-Soli R. 1986; Characterization of cellulase and xylanase activities of Streptomyces lividans . Applied and Microbial Biotechnology 24:230–234
    [Google Scholar]
  16. Mccarthy A.J. 1987; Lignocellulose degrading actinomycetes. FEMS Microbiology Reviews 46:145–163
    [Google Scholar]
  17. Malfait M., Godden B., Penninckx M. 1984; Growth and cellulase production of Micromonospora chalcae and Pseudonocardia thermophila . Annales de microbiologie 135B:79–89
    [Google Scholar]
  18. Miller G.L. 1959; Use of dinitrosalicylic acid reagent for determination of reducing sugar. Analytical Chemistry 31:426–428
    [Google Scholar]
  19. Theater R.M., Wood P.J. 1982; Use of Congo Red-polysaccharide interactions in enumeration and characterization of cellulolytic bacteria from the bovine rumen. Applied and Environmental Microbiology 43:777–780
    [Google Scholar]
  20. Updegraaf D.M. 1969; Semi-micro determination of cellulose in biological materials. Analytical Chemistry 32:420–424
    [Google Scholar]
  21. Vanzyl W.H. 1985; A study of the cellulase produced by three mesophilic actinomycetes grown on bagasse as substrate. Biotechnology and Bioengineering 27:1367–1373
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-135-2-285
Loading
/content/journal/micro/10.1099/00221287-135-2-285
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error