We investigated eight serine pathway mutants of AM1; one lacked serine glyoxylate aminotransferase, four were defective in glycerate kinase, and three were blocked in the unknown part of the serine pathway which involves conversion of acetyl-CoA to glyoxylate. We complemented six of these mutants using a gene library of AM1 DNA and identified two new regions of the chromosome which encode polypeptides necessary for C-1 assimilation. One is an 8·8 kbp III fragment required for the production of glycerate kinase and the other a III fragment of approximately 13 kbp essential for the conversion of acetyl-CoA to glyoxylate. These new C-1 regions of the chromosome are not closely linked to the previously described methanol oxidation genes, nor to a gene required for the production of an active malyl-CoA lyase. Our mutants were also complemented by clones from a gene library of a related pink pigmented facultative methylotroph, .


Article metrics loading...

Loading full text...

Full text loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error