1887

Abstract

We investigated eight serine pathway mutants of AM1; one lacked serine glyoxylate aminotransferase, four were defective in glycerate kinase, and three were blocked in the unknown part of the serine pathway which involves conversion of acetyl-CoA to glyoxylate. We complemented six of these mutants using a gene library of AM1 DNA and identified two new regions of the chromosome which encode polypeptides necessary for C-1 assimilation. One is an 8·8 kbp III fragment required for the production of glycerate kinase and the other a III fragment of approximately 13 kbp essential for the conversion of acetyl-CoA to glyoxylate. These new C-1 regions of the chromosome are not closely linked to the previously described methanol oxidation genes, nor to a gene required for the production of an active malyl-CoA lyase. Our mutants were also complemented by clones from a gene library of a related pink pigmented facultative methylotroph, .

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1989-02-01
2022-01-24
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