1887

Abstract

Tn differs in size and restriction map from the well-characterized Tn. It also differs in its preferential and high-frequency insertion into the 6 kb I-C region of RP1. The affinity for this region is perpetuated in pVS76, a clone of RP1 I-C in pBR322. Restriction mapping of independent pVS76::Tn derivatives revealed that Tn inserted at the same site (or small region) in I-C corresponding to the 35 kb coordinate in RP1. Insertion occurred in both orientations, but one was preferred. When I-C was deleted from RP1, acquisition of Tn was reduced and the sites of insertion randomized.

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1989-11-01
2021-05-14
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