1887

Abstract

The ability of selected bacterial cultures to synthesize ethylene during growth in nutrient broth supplemented with methionine or 2-oxo-4-methylthiobutyric acid (KMBA) was examined. Although most cultures transformed KMBA into ethylene, only those of SPAO and were able to convert exogenously added methionine to ethylene. In chemically defined media, SPAO produced the highest amounts of ethylene from methionine and KMBA. This capability was affected by the nature of the carbon source and the type and amount of nitrogen source used for growth. When glutamate was used as sole source of carbon and nitrogen for growth, the activity of the ethylenogenic enzymes was reduced to 25% of that observed with cultures grown with glucose and NHCl. Neither methionine nor KMBA significantly affected the ethylenogenic capacity of SPAO. Menadione and paraquat, compounds that generate superoxide radicals, stimulated ethylene synthesis by harvested cells, but not by cell-free extracts of SPAO. In addition, cells of , which produced no ethylene in culture in the presence of exogenously added KMBA, yet possessed the necessary enzymes in an active form, were able to synthesize ethylene from KMBA when incubated with menadione or paraquat.

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1989-11-01
2021-10-16
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References

  1. Adams D. O., Yang S. F. 1979; Ethylene biosynthesis: identification of 1-aminocyclopro- pane-l-carboxylic acid as an intermediate in the conversion of methionine to ethylene. Proceedings of the National Academy of Sciences of the United States of America 76:170–174
    [Google Scholar]
  2. Adams D. O., Yang S. F. 1981; Ethylene the gaseous plant hormone: mechanism and regulation of biosynthesis. Trends in Biochemical Sciences 6:161–164
    [Google Scholar]
  3. Billington D. C., Golding B. T., Primrose S. B. 1979; Biosynthesis of ethylene from methionine. Isolation of the putative intermediate 4-methylthio- 2-oxobutanoate from culture fluids of bacteria and fungi. Biochemical Journal 82:827–836
    [Google Scholar]
  4. Fawcett J. K., Scott J. E. 1960; A rapid and precise method for the determination of urea. Journal of Clinical Pathology 13:156–159
    [Google Scholar]
  5. Feierman D. E., Cederbaum A. I. 1985; Ethylene production from a-keto-4-thiomethylbutyric acid by isolated rat liver cells. Suspension medium, and perfusates in the absence and presence of iron. Journal of Free Radicals in Biology & Medicine 1:155–162
    [Google Scholar]
  6. Hemming B. C., Gubler C. J. 1979; High-pressure liquid chromatography of a-keto acid-dinitro- phenylhydrazones. Analytical Biochemistry 92:3140
    [Google Scholar]
  7. Ince J. E., Knowles C. J. 1985; Ethylene formation by cultures of Escherichia coli. Archives of Microbiology 141:209–213
    [Google Scholar]
  8. Ince J. E., Knowles C. J. 1986; Ethylene formation by cell-free extracts of Escherichia coli. Archives of Microbiology 146:151–158
    [Google Scholar]
  9. Knowles C. J., Bunch A. W. 1986; Microbial cyanide metabolism. Advances in Microbial Physiology 27:73–106
    [Google Scholar]
  10. Primrose S. B. 1976 Formation of ethylene by Escherichia coli Journal of General Microbiology 95:159–165
    [Google Scholar]
  11. Primrose S. B. 1977; Evaluation of the role of methional, KMBA and peroxidase in ethylene formation by Escherichia coli. Journal of General Microbiology 98:519–528
    [Google Scholar]
  12. Primrose S. B. 1979; Ethylene and agriculture: the role of the microbe. Journal of Applied Bacteriology 46:1–25
    [Google Scholar]
  13. Primrose S. B., Dilworth M. J. 1976; Ethylene production by bacteria. Journal of General Microbiology 93:177–181
    [Google Scholar]
  14. Yang S. F. 1969; Further studies on ethylene formation from a-keto-y-methylthiobutyric acid or β- methylthiopropinoaldehyde by peroxidase in the presence of sulphide and oxygen. Journal of Biological Chemistry 244:4360–4365
    [Google Scholar]
  15. Yang S. F. 1980; Regulation of ethylene biosynthesis. Horticultural Science 15:238–243
    [Google Scholar]
  16. Yang S. F. 1985; Biosynthesis and action of ethylene. Horticultural Science 20:41–45
    [Google Scholar]
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