1887

Abstract

Phosphoprotein patterns in two mutants of (ts) and (ts) , were analysed by two-dimensional polyacrylamide gel electrophoresis. Comparison with the phosphoprotein patterns of the mutants (ts) and , analysed in a previous study, demonstrated not only that the gene product is a positive element in the regulation of adenylyl cyclase activity, as suggested by recent studies, but that it is also a negative element in the phosphorylation of a 31 kDa protein (p31c and p31d), a protein whose phosphorylation is correlated with cell cycle arrest, and dephosphorylation with cell cycle initiation, respectively. Moreover, the phosphorylation phenotype of p31c and p31d suggests that the activity of the protein is subject to feedback regulation by cAMP-dependent protein kinase, and that the protein is a key element in an ammonium (NH ) signal-response system.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-134-9-2481
1988-09-01
2024-04-26
Loading full text...

Full text loading...

/deliver/fulltext/micro/134/9/mic-134-9-2481.html?itemId=/content/journal/micro/10.1099/00221287-134-9-2481&mimeType=html&fmt=ahah

References

  1. Broek D., Toda T., Michaeli T., Levin L., Birchmeier C., Zoller M., Powers S., Wlgler M. 1987; The S. cerevisiae CDC25 gene product regulates the RAS/adenylate cyclase pathway. Cell 48:789–799
    [Google Scholar]
  2. Camonis J. H., Kalekine M., Gondri B., Garreau H., Boy-Marcotte E., Jaquet M. 1986; Characterization, cloning and sequence analysis of the CDC25 gene which controls the cyclic AMP level of Saccharomyces cerevisiae . EMBO Journal 5:375–380
    [Google Scholar]
  3. Daniel J., Becker J. M., Enari E., Levitski A. 1987; The activation of adenylate cyclase by guanyl nucleotides in Saccharomyces cerevisiae is controlled by the CDC25 start gene product. Molecular and Cellular Biology 7:3857–3861
    [Google Scholar]
  4. Kataoka T., Powers S., Mcgill C., Fasano O., Strathern J., Broach J., Wigler M. 1984; Genetic analysis of yeast RAS1 and RAS2 genes. Cell 37:437–445
    [Google Scholar]
  5. Lisziewicz J., Godany A., Forster H.-H., Kuntzel H. 1987; Isolation and nucleotide sequence of a Saccharomyces cerevisiae protein kinase gene that suppresses the cell cycle start mutation cdc25. Journal of Biological Chemistry 262:2549–2553
    [Google Scholar]
  6. Martegani E., Baroni M., Vanoni M. 1986; Interaction of cAMP with the CDC25-mediated step in the cell cycle of budding yeast. Experimental Cell Research 162:544–548
    [Google Scholar]
  7. Matsumoto K., Uno I., Ishikawa T. 1983; Initiation of meiosis in yeast mutants defective in adenylate cyclase and cyclic AMP-dependent protein kinase. Cell 32:417–423
    [Google Scholar]
  8. Matsumoto K., Uno I., Ishikawa T. 1984; Identification of the structural gene and nonsense alleles for adenylate cyclase in Saccharomyces cerevisiae . Journal of Bacteriology 157:277–282
    [Google Scholar]
  9. Matsumoto K., Uno I., Ishikawa T. 1985; Genetic analysis of the role of cAMP in yeast. Yeast 1:15–24
    [Google Scholar]
  10. Nikawa J., Cameron S., Toda T., Ferguson K. M., Wigler M. 1987; Rigorous feedback control of cAMP levels in Saccharomyces cerevisiae . Genes and Development 1:931–937
    [Google Scholar]
  11. O’Farrell . 1975; High resolution two-dimensional electrophoresis of proteins. Journal of Biological Chemistry 250:4007–4021
    [Google Scholar]
  12. Olempska-Beer Z., Freese E. 1987; Initiation of meiosis and sporulation in Saccharomyces cerevisiae does not require a decrease in cyclic AMP. Molecular and Cellular Biology 7:2141–2147
    [Google Scholar]
  13. Portillo F., Mazon M. J. 1986; The yeast start mutant cdc25 is defective in the activation of plasma membrane ATPase by glucose. Journal of Bacteriology 168:1254–1257
    [Google Scholar]
  14. Reed S. I. 1980; The selection of Saccharomyces cerevisiae mutants defective in the start event of cell division. Genetics 95:561–577
    [Google Scholar]
  15. Robinson L. C., Gibbs J. B., Marshall M. S., Sigal I. S., Tatchell K. 1987; CDC25: a component of the RAS-adenylate cyclase pathway in Saccharomyces cerevisiae . Science 235:1218–1221
    [Google Scholar]
  16. Shilo V., Simchen G., Shilo B. 1978; Initiation of meiosis in cell-cycle initiation mutants of Saccharomyces cerevisiae . Experimental Cell Research 112:241–248
    [Google Scholar]
  17. Sibley D., Benovic J., Caron M., Lefkowitz R. 1987; Regulation of transmembrane signaling by receptor phosphorylation. Cell 48:913–922
    [Google Scholar]
  18. Toda T., Uno I., Ishikawa T., Powers S., Kataoka T., Broek D., Cameron S., Broach J., Matsumoto K., Wigler M. 1985; In yeast, RAS proteins are controlling elements of adenylate cyclase. Cell 40:27–36
    [Google Scholar]
  19. Tripp M. L., Piñon R. 1986; Control of the cAMP pathway by the cell cycle start function, CDC25, in Saccharomyces cerevisiae . Journal of General Microbiology 132:1143–1151
    [Google Scholar]
  20. Tripp M. L., Piñon R., Meisenhelder J., Hunter T. 1986; Identification of phosphoproteins correlated with proliferation and cell cycle arrest in Saccharomyces cerevisiae: positive and negative regulation by cAMP-dependent protein kinase. Proceedings of the National Academy of Sciences of the United States of America 83:5973–5977
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-134-9-2481
Loading
/content/journal/micro/10.1099/00221287-134-9-2481
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error