1887

Abstract

An obligately symbiotic bacterium, strain T, associating with a specific strain S of a thermophilic sp. was isolated during screening for micro-organisms that produce heat-stable tryptophanase and -tyrosinase. A colony containing both strains was finally isolated by successive selection with bacitracin. In mixed culture with strain S under low aeration conditions at 60 °C, growth of strain T was initiated following lysis of the cells. No independent growth of strain T was observed in any of the media tested, even in the presence of other thermophilic strains or cell-free extracts of strain S or its killed cells. No physical adhesion between cells of strains S and T was observed microscopically. Tryptophanase and β-tyrosinase production by strain T were induced by -tryptophan and L-tyrosine, respectively. The name gen. nov., sp. nov. is proposed for strain T which is a Gram-negative rod (0·25–0·35 µm diameter) with a multilayer surface structure containing meso-diaminopimelic acid. The G+C content of the DNA is 65·1 mol%; iso-C, iso-c and anteiso-C acids are the major cellular fatty acids. The type strain is strain IAM 13621.

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/content/journal/micro/10.1099/00221287-134-8-2353
1988-08-01
2022-01-21
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