SUMMARY: Earlier studies on the fermentation of D-[1-C]- and D-[3,4-C]glucose by showed that lactate was the major fermentation product and that it was probably produced by glycolysis. It was therefore recommended that be reclassified as a homofermentative organism. In the present investigation, products of D-[1-C]-, D-[2-C]- and D-[3,4-C]glucose fermented by were isolated, and their specific radioactivities and the distribution patterns of radioactivity in their C-atoms were determined. The positional labelling patterns of the fermentation products, their specific radioactivities and their concentrations confirmed that glucose is degraded via the glycolytic pathway. Some secondary decarboxylation/dissimilation of pyruvate to acetate, formate and CO was also observed. These results provide conclusive proof that is indeed a homofermentative organism. Results obtained with D-[U-C]glucose showed that approximately three-quarters of the lactate but less than 10% each of the formate and acetate were produced from glucose. The remainder was presumably derived to a varying degree from endogenous non-glucose sources such as fructose and/or amino acids.


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