1887

Abstract

Earlier studies on the fermentation of D-[1-C]- and -[3,4-C]glucose by showed that lactate was the major fermentation product and that it was probably produced by glycolysis. It was therefore recommended that be reclassified as a homofermentative organism. In the present investigation, products of -[1-C]-, -[2-C]- and -[3,4-C]glucose fermented by were isolated, and their specific radioactivities and the distribution patterns of radioactivity in their C-atoms were determined. The positional labelling patterns of the fermentation products, their specific radioactivities and their concentrations confirmed that glucose is degraded via the glycolytic pathway. Some secondary decarboxylation/dissimilation of pyruvate to acetate, formate and CO was also observed. These results provide conclusive proof that is indeed a homofermentative organism. Results obtained with -[U-C]glucose showed that approximately three-quarters of the lactate but less than 10 % each of the formate and acetate were produced from glucose. The remainder was presumably derived to a varying degree from endogenous non-glucose sources such as fructose and/or amino acids.

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1988-08-01
2021-05-12
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References

  1. Abo-Elnaga I. G., Kandler O. 1965; Zur Taxonomie der Gattung Lactobacillus Beijerink.2. Das Subgenus Betabacterium Orla Jensen. Zentralblat für Bakteriologie, Parasitenkunde, Infectionskrank- heiten und Hygiene, 2. Abt. 119:117–129
    [Google Scholar]
  2. Bergmeyer H. U., MÖllering H. 1974; Methods for determination of metabolites:acetate. In Methods of Enzymatic Analysis, 2nd edn.. pp. 1520–1528 Bergmeyer H. U. Edited by New York& London: Academic Press;
    [Google Scholar]
  3. Bernt E., Bergmeyer H. U. 1974; Methods for determination of metabolites :d-fructose. In Methods of Enzymatic Analysis, 2nd edn.. pp. 1304–1307 Bergmeyer H. U. Edited by New York & London: Academic Press;
    [Google Scholar]
  4. Cluley H. J. 1962; Suspension scintillation counting of carbon-14 barium carbonate. Analyst 87:170–177
    [Google Scholar]
  5. Dawes E. A. 1980; Determination of metabolic pathways. In Quantitative Problems in Biochemistry, 6th edn. pp. 248–267 London & New York:: Longman.;
    [Google Scholar]
  6. De Bruyn I. N., Louw A. I., Visser L., Holzapfel W. H. 1987; Lactobacillus divergens is a homofermentative organism. Systematic and Applied Microbiology 9:173–175
    [Google Scholar]
  7. Entner N., Doudoroff M. 1952; Glucose and gluconic acid oxidation of Pseudomonas saccharophila . Journal of Biological Chemistry 196:853–862
    [Google Scholar]
  8. Gottschalk G. 1979; Bacterial fermentation. In Bacterial Metabolism pp. 218–219 New York:: Springer-Verlag.;
    [Google Scholar]
  9. Guerrant G. O., Lambert M. A., Moss C. W. 1982; Analysis of short chain acids from anaerobic bacteria by high performance liquid chromatography. Journal of Clinical Microbiology 16:355–360
    [Google Scholar]
  10. Holzapfel W. H., Gerber E. S. 1983; Lactobacillus divergens sp. nov., a new heterofermentative Lactobacillus species producing l( + )-lactate. Systematic and Applied Microbiology 4:522–534
    [Google Scholar]
  11. Kandler O., Weiss N. 1986; Genus Lactobacillus . In Bergey’s Manual of Systematic Bacteriology 11 pp. 1209–1234 Sneath P. H. A. Edited by Baltimore: Williams & Wilkins;
    [Google Scholar]
  12. Noll F. 1974; Methods for determination of metabolites :l-( + )-lactate. In Methods of Enzymatic Analysis, 2nd edn.. pp. 1475–1479 Bergmeyer H. U. Edited by New York & London: Academic Press;
    [Google Scholar]
  13. Sharpe M. E. 1981; The Genus Lactobacillus . In The Prokaryotes pp. 1653–1679 Starr M. P., Stolp H., Trüper H. G., Balows A., schlegel H. G. Edited by Berlin: Springer-Verlag;
    [Google Scholar]
  14. Stams A. J. M., Hansen T. A., Skyring G. W. 1985; Utilization of amino acids as energy substrates by two marine Desulfovibrio strains. FEMS Microbiology Ecology 31:11–15
    [Google Scholar]
  15. Williams J. F., Rienits K. G., schofield P. J., Clark M. G. 1971; The pentose phosphate pathway in rabbit liver. Biochemical Journal 123:923–943
    [Google Scholar]
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