1887

Abstract

Earlier studies on the fermentation of D-[1-C]- and -[3,4-C]glucose by showed that lactate was the major fermentation product and that it was probably produced by glycolysis. It was therefore recommended that be reclassified as a homofermentative organism. In the present investigation, products of -[1-C]-, -[2-C]- and -[3,4-C]glucose fermented by were isolated, and their specific radioactivities and the distribution patterns of radioactivity in their C-atoms were determined. The positional labelling patterns of the fermentation products, their specific radioactivities and their concentrations confirmed that glucose is degraded via the glycolytic pathway. Some secondary decarboxylation/dissimilation of pyruvate to acetate, formate and CO was also observed. These results provide conclusive proof that is indeed a homofermentative organism. Results obtained with -[U-C]glucose showed that approximately three-quarters of the lactate but less than 10 % each of the formate and acetate were produced from glucose. The remainder was presumably derived to a varying degree from endogenous non-glucose sources such as fructose and/or amino acids.

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1988-08-01
2024-05-10
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