Construction of Multicopy Expression Vectors for Regulated Over-production of Proteins in Klebsiella pneumoniae and Other Enteric Bacteria

References

1. Amann E., Brosius J., Ptashne M. 1983; Vectors bearing a hybrid trp-lac promoter useful for regulated expression of cloned genes in Escherichia coli. Gene 25:167–178
   [Google Scholar]
2. Austin S., Henderson N., Dixon R. 1987; Requirements for transcriptional activation in vitro of the nitrogen-regulated glnA and nifLA promoters from Klebsiella pneumoniae: dependence on activator concentration. Molecular Microbiology 1:92–100
   [Google Scholar]
3. Casadaban M., Cohen S. N. 1980; Analysis of gene control signals by DNA fusion and cloning in Escherichia coli. Journal of Molecular Biology 138:179–207
   [Google Scholar]
4. Chang A., Cohen S. N. 1978; Construction and characterisation of amplifiable multicopy DNA cloning vehicles derived from the pl5A cryptic miniplasmid. Journal of Bacteriology 134:1141–1156
   [Google Scholar]
5. Holmes D. S., Quigley M. 1981; A rapid boiling method for the preparation of bacterial plasmids. Analytical Biochemistry 114:193–197
   [Google Scholar]
6. Hunt T., Magasanik B. 1985; Transcription of glnA by purified Escherichia coli components: core RNA polymerase and the products of glnF, glnG and glnL. Proceedings of the National Academy of Sciences of the United States of America 82:8453–8457
   [Google Scholar]
7. Maniatis T., Fritsch E. F., Sambrook J. 1982 Molecular Cloning. A Laboratory Manual Cold Spring Harbor, NY: Cold Spring Harbor Laboratory;
   [Google Scholar]
8. Merrick M. J., Gibbins J. R. 1985; The nucleotide sequence of the nitrogen-regulation gene ntrA of Klebsiella pneumoniae and comparison with conserved features in bacterial RNA polymerase sigma factors. Nucleic Acids Research 13:7607–7620
   [Google Scholar]
9. Merrick M. J., Gibbins J. R., Postgate J. R. 1987; A rapid and efficient method for plasmid transformation of Klebsiella pneumoniae and Escherichia coli. Journal of General Microbiology 133:2053–2057
   [Google Scholar]
10. Müller-Hill B., Crapo L., Gilbert W. 1968; Mutants that make more lac repressor. Proceedings of the National Academy of Sciences of the United States of America 59:1259–1264
    [Google Scholar]
11. Paul W., Merrick M. 1987; The nucleotide sequence of the nifM gene of Klebsiella pneumoniae and identification of a new nif gene - nifZ. European Journal of Biochemistry 170:259–265
    [Google Scholar]
12. Pouwels PH., Enger-Valk B. E., Brammar W. J. 1985 Cloning Vectors. A Laboratory Manual Amsterdam: Elsevier;
    [Google Scholar]
13. Remaut E., Stanssens P., Fiers W. 1981; Plasmid vectors for high-efficiency expression controlled by the p_L promoter of coliphage lambda. Gene 15:81–93
    [Google Scholar]
14. Spratt B. G., Hedge P. J., Te Heesen S., Edelman A., Broome-Smith J. K. 1986; Kanamycin- resistant vectors that are analogues of plasmids pUC8, pUC9, pEMBL8 and pEMBL9. Gene 41:337–342
    [Google Scholar]
15. Stark M. J. R. 1987; Multicopy expression vectors carrying the lac repressor gene for regulated high- level expression of genes in Escherichia coli. Gene 51:255–267
    [Google Scholar]