1887

Abstract

An analysis of the genes governing the osmoregulatory choline-glycine betaine pathway of was performed. A 9 kb HI fragment, located 30 to 39 kb counterclockwise of the RI site of , coded for all known Bet activities. The following genes were identified: the gene for the choline dehydrogenase, the gene for the betaine aldehyde dehydrogenase, and the gene or operon for the high-affinity choline transport. The and the genes were named in this paper, and the clockwise gene order was shown to be Subcloning gave plasmids which expressed each of the three Bet activities separately. The cloned genes remained osmotically regulated, indicating the existence of several osmotically regulated promoters in the region. , which carried the region of in the broad-host-range vector pRK293 expressed the three Bet activities and displayed increased osmotic tolerance in the presence of choline.

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1988-06-01
2022-01-22
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References

  1. Adams C. W., Hatfield G. W. 1984; Effects of promoter strengths and growth conditions on copy number of transcription-fusion vectors. Journal of Biological Chemistry 259:7399–7403
    [Google Scholar]
  2. Ahmad S. I., Pritchard R. H. 1972; Location of the gene specifying cytosine deaminase in Escherichia coli. Molecular and General Genetics 118:323–325
    [Google Scholar]
  3. Bochner B. R., Huang H. C., Schieven G. L. 1980; Positive selection for loss of tetracycline resistance. Journal of Bacteriology 143:926–933
    [Google Scholar]
  4. Bolivar F., Rodriguez R. L., Greene P. J., Betlach M. C., Heyneker H. L., Boyer H. W., Crosa J. H., Falkow S. 1977; Construction and characterization of new cloning vehicles. II. A multipurpose cloning system. Gene 2:95–113
    [Google Scholar]
  5. Cairney J., Booth I. R., Higgins C. F. 1985a; Salmonella typhimurium proP gene encodes a transport system for the osmoprotectant betaine. Journal of Bacteriology 164:1218–1223
    [Google Scholar]
  6. Cairney J., Booth I. R., Higgins C. F. 1985b; Osmoregulation of gene expression in Salmonella typhimurium: proU encodes an osmotically induced betaine transport system. Journal of Bacteriology 164:1224–1232
    [Google Scholar]
  7. Csonka L. N. 1981; Proline over-production results in enhanced osmotolerance in Salmonella typhimurium. Molecular and General Genetics 182:82–86
    [Google Scholar]
  8. Ditta G., Schmidhauser T., Yakobson E., Lu P., Liang X. W., Finlay D. R., Guiney D., Helinski D. R. 1985; Plasmids related to the broad host range vector, pRK290, useful for gene cloning and for monitoring gene expression. Plasmid 13:149–153
    [Google Scholar]
  9. Epstein W. 1986; Osmoregulation by potassium transport in Escherichia coli. FEMS Microbiology Reviews 39:73–78
    [Google Scholar]
  10. Galinski E. A., Trüper H. G. 1982; Betaine, a compatible solute in the extremely halophilic bacterium Ectothiorhodospira halochloris. FEMS Micro-biology Letters 13:357–360
    [Google Scholar]
  11. Gowrishankar J., Jayashree P., Rajkumari K. 1986; Molecular cloning of an osmoregulatory locus in Escherichia coli: increased proU gene dosage results in enhanced osmotolerance. Journal of Bacteriology 168:1197–1204
    [Google Scholar]
  12. Hadley R. G., Hu M., Timmons M., Yun K., Deonier R. C. 1983; A partial restriction map of the proA-purE region of the Escherichia coli K-12 chromosome. Gene 22:281–287
    [Google Scholar]
  13. Higgins C. F., Sutherland L., Cairney J., Booth I. R. 1987; The osmotically regulated proU locus of Salmonella typhimurium encodes a periplas- mic betaine-binding protein. Journal of General Microbiology 133:305–310
    [Google Scholar]
  14. Hohn B. 1979; In vitro packaging of λ and cosmid DNA. Methods in Enzymology 68:299–309
    [Google Scholar]
  15. Imhoff J. F., Rodriguez-Valera F. 1984; Betaine is the main compatible solute of halophilic eubacteria. Journal of Bacteriology 160:478–479
    [Google Scholar]
  16. Jakowec M. W., Smith L. T., Dandekar A. M. 1985; Recombinant plasmid conferring proline overproduction and osmotic tolerance. Applied and Environmental Microbiology 50:441–446
    [Google Scholar]
  17. Landfald B., Strøm A. R. 1986; Choline-glycine betaine pathway confers a high level of osmotic tolerance in Escherichia coli. Journal of Bacteriology 165:849–855
    [Google Scholar]
  18. Larsen P. I., Sydnes L. K., Landfald B., Strøm A. R. 1987; Osmoregulation in Escherichia coli by accumulation of organic osmolytes: betaines, glutamic acid, and trehalose. Archives of Microbiology 147:1–7
    [Google Scholar]
  19. Le Rudulier D., Strøm A. R., Dandekar A. M., Smith L. T., Valentine R. C. 1984; Molecular biology of osmoregulation. Science 224:1064–1068
    [Google Scholar]
  20. Maniatis T., Fritsch E. F., Sambrook J. 1982 Molecular Cloning. A Laboratory Manual Cold Spring Harbor, New York: Cold Spring Harbor Laboratory;
    [Google Scholar]
  21. May G., Faatz E., Villarejo M., Bremer E. 1986; Binding protein dependent transport of glycine betaine and its osmotic regulation in Escherichia coli K12. Molecular and General Genetics 205:225–233
    [Google Scholar]
  22. Miller J. H. 1972 Experiments in Molecular Genetics Cold Spring Harbor, New York: Cold Spring Harbor Laboratory;
    [Google Scholar]
  23. Reed R. H., Borowitzka L. J., Mackay M. A., Chudek J. A., Foster R., Warr S. R. C., Moore D. J., Stewart W. D. P. 1986; Organic solute accumulation in osmotically stressed cyanobacteria. FEMS Microbiology Reviews 39:51–56
    [Google Scholar]
  24. Roberts I., Mountford R., High N., Bitter-Suermann D., Jann K., Tlmmis K., Boulnois G. 1986; Molecular cloning and analysis of genes for production of K5, K7, K12, and K92 capsular polysaccharides in Escherichia coli. Journal of Bacteriology 168:1228–1233
    [Google Scholar]
  25. Shkedy-Vinkler C., Avi-DOR Y. 1975; Betaine induced stimulation of respiration at high osmolarities in a halotolerant bacterium. Biochemical Journal 150:219–226
    [Google Scholar]
  26. Simon R., Priefer U., Pühler A. 1983; A broad host range mobilization system for in vivo genetic engineering: transposon mutagenesis in gram negative bacteria. Biotechnology 1:784–791
    [Google Scholar]
  27. Soberon X., Covarrubias L., Bolivar F. 1980; Construction and characterization of new cloning vehicles. IV. Deletion derivatives of pBR 322 and pBR 325. Gene 9:287–305
    [Google Scholar]
  28. Stoker N. G., Fairweather N. F., Spratt B. G. 1982; Versatile low-copy-number plasmid vectors for cloning in Escherichia coli. Gene 18:335–341
    [Google Scholar]
  29. Strøm A. R., Falkenberg P., Landfald B. 1986; Genetics of osmoregulation in Escherichia coli: uptake and biosynthesis of organic osmolytes. FEMS Microbiology Reviews 39:79–86
    [Google Scholar]
  30. Styrvold O. B., Falkenberg P., Landfald B., Eshoo M. W., Bjørnsen T., Strøm A. R. 1986; Selection, mapping, and characterization of osmoregulatory mutants of Escherichia coli blocked in the choline-glycine betaine pathway. Journal of Bacteriology 165:856–863
    [Google Scholar]
  31. Sutherland L., Cairney J., Elmore M. J., Booth I. R., Higgins C. F. 1986; Osmotic regulation of transcription: induction of the proU betaine transport gene is dependent on accumulation of intracellular potassium. Journal of Bacteriology 168:805–814
    [Google Scholar]
  32. Tsuge H., Futamura Y., Nakano Y., Ohashi K. 1980; Rat liver mitochondrial choline dehydrogenase. Estimation of molecular weight. Biochemistry International 1:519–525
    [Google Scholar]
  33. Yancey P. H., Clark M. E., Hand S. C., Bowlus R. D., Somero G. N. 1982; Living with water stress: evolution of osmolyte systems. Science 217:1214–1222
    [Google Scholar]
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