1887

Abstract

The SDS-PAGE patterns of the iron-regulated outer-membrane proteins from 70 strains of isolated from various human and animal infections were analysed and the nature of the siderophores produced was examined. Iron-regulated 81 kDa and 74 kDa protein bands seen in SDS-PAGE gels were characterized further by immunoblotting using anti-81 kDa and anti-74 kDa (Cir) sera. The results showed considerable differences between the patterns of the iron-regulated outer-membrane proteins exhibited by the different strains. Nevertheless, three distinct and characteristic profiles, based on the most prominent bands expressed, could be identified, although not all strains produced patterns which matched with one of these. These results suggest the possibility of using the pattern of iron-regulated outer-membrane proteins expressed, as well as siderophores produced, as a new set of markers to characterize groups of pathogenic

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-134-6-1549
1988-06-01
2021-10-26
Loading full text...

Full text loading...

/deliver/fulltext/micro/134/6/mic-134-6-1549.html?itemId=/content/journal/micro/10.1099/00221287-134-6-1549&mimeType=html&fmt=ahah

References

  1. Arnow L. E. 1937; Colorimetric determination of the components of 3,4-dihydroxyphenylalanine- tyrosine mixtures. Journal of Biological Chemistry 228:531–537
    [Google Scholar]
  2. Achtman M., Pluschke G. 1986; Clonal analysis of descent and virulence among selected Escherichia coli . Annual Review of Microbiology 40:185–210
    [Google Scholar]
  3. Achtman M., Mercer A., Kusecek B., Pohl A., Heuzenroeder M., Aaronson W., Sutton A., Silver R. P. 1983; Six widespread bacterial clones among Escherichia coli K1 isolates. Infection and Immunity 39:315–335
    [Google Scholar]
  4. Achtman M., Heuzenroeder M., Kusecek B., Ochman H., Caugant D., Selander R. K., Vaisanen-Rhen V., Korhonen T. K., Stuart S., Ørskov F., Ørskov I. 1986; Clonal analysis of Escherichia coli 02:K1 isolated from diseased humans and animals. Infection and Immunity 51:268–276
    [Google Scholar]
  5. Atkin C. L., Neilands J. B., Phaff H. J. 1970; Rhodotorulic acid from species of Leucosporidium, Rhodosporidium, Rhodotorula, Sporidiobolus and Sporobolomyces and a new alanine-containing ferrichrome from Cryptococcus melibiosium . Journal of Bacteriology 103:722–733
    [Google Scholar]
  6. Bindereif A., Braun V., Hantke K. 1982; The cloacin receptor of ColV-bearing Escherichia coli is part of the Fe3+-aerobactin transport system. Journal of Bacteriology 150:1472–1475
    [Google Scholar]
  7. Braun V. 1981; Escherichia coli cells containing the plasmid ColV produce the ionophore aerobactin. FEMS Microbiology Letters 11:225–228
    [Google Scholar]
  8. Carbonetti N. H., Boonchai S., Parry S. H., Vaisanen-Rhen V., Korhonen T. K., Williams P. H. 1986; Aerobactin-mediated iron uptake by Escherichia coli isolates from human extra-intestinal infections. Infection and Immunity 51:966–968
    [Google Scholar]
  9. Chart H., Griffiths E. 1985; Antigenic and molecular homology of the ferric enterobactin receptor protein of Escherichia coli . Journal of General Microbiology 131:1503–1509
    [Google Scholar]
  10. Filip C., Fletcher G., Wulff J. L., Earhart C. F. 1973; Solubilization of the cytoplasmic membrane of Escherichia coli by the ionic detergent sodium lauryl sarcosinate. Journal of Bacteriology 115:717–722
    [Google Scholar]
  11. Grewal K. K., Warner P. J., Williams P. H. 1982; An inducible outer membrane protein involved in aerobactin mediated iron transport by ColV strains of Escherichia coli . FEBS Letters 140:27–30
    [Google Scholar]
  12. Griffiths E. 1983; Adaptation and multiplication of bacteria in host tissues. Philosophical Transactions of the Royal Society B303:85–96
    [Google Scholar]
  13. Griffiths E. 1987; The iron-uptake systems of . pathogenic bacteria. In Iron and Infection; Molecular, Physiological and Clinical Aspects pp 69–137 Bullen J. J., Griffiths E. Edited by Chichester: Wiley;
    [Google Scholar]
  14. Griffiths E., Stevenson P., Joyce P. 1983; Pathogenic Escherichia coli express new outer membrane proteins when growing in vivo . FEMS Microbiology Letters 16:95–99
    [Google Scholar]
  15. Griffiths E., Stevenson P., Thorpe R., Chart H. 1985a; Naturally occuring antibodies in human sera that react with the iron regulated outer membrane proteins of Escherichia coli . Infection and Immunity 47:808–813
    [Google Scholar]
  16. Griffiths E., Stevenson P., Hale T. L., Formal S. B. 1985b; Synthesis of aerobactin and a 76000- dalton iron-regulated outer membrane protein by Escherichia coli Kl2-Shigellaflexneri hybrids and by enteroinvasive strains of Escherichia coli . Infection and Immunity 49:67–71
    [Google Scholar]
  17. Hollifield W. C. Jr Fiss E. H., Neilands J. B. 1978; Modification of a ferric enterobactin receptor protein from the outer membrane of Escherichia coli . Biochemical and Biophysical Research Communications 83:739–746
    [Google Scholar]
  18. Korhonen T. K., Valtonen M. V., Parkkinen J., Vaisanen-Rhen V., Finne J., Ørskov F., Ørskov I., Svenson S. B., Makela P. H. 1985; Serotypes, hemolysin production and receptor recognition of Escherichia coli strains associated with neonatal sepsis and meningitis. Infection and Immunity 48:486–491
    [Google Scholar]
  19. Lawlor K. M., Daskaleros P. A., Robinson R. E., Payne S. M. 1987; Virulence of iron transport mutants of Shigella flexneri and utilisation of host iron compounds. Infection and Immunity 55:594–599
    [Google Scholar]
  20. Linggood M. A., Roberts M., Ford S., Parry S. H., Williams P. H. 1987; Incidence of the aerobactin iron uptake system among Escherichia coli isolates from infections of farm animals. Journal of General Microbiology 133:835–842
    [Google Scholar]
  21. Marolda C. L., Valvano M. A., Lawlor K. M., Payne S. M., Crosa J. H. 1987; Flanking and internal regions of chromosomal genes mediating aerobactin iron uptake systems in enteroinvasive Escherichia coli and Shigella flexneri . Journal of General Microbiology 133:2269–2278
    [Google Scholar]
  22. Montgomerie J. Z., Bindereif A., Neilands J. B., Kahmanson G. M., Guze L. B. 1984; Association of hydroxamate siderophore (aerobactin) with Escherichia coli isolated from patients with bacteremia. Infection and Immunity 46:835–838
    [Google Scholar]
  23. Nassif X., Mazert M. C., Mounier J., Sansonetti P. J. 1987; Evaluation with an iuc::Tnl0 mutant of the role of aerobactin production in the virulence of Shigella flexneri . Infection and Immunity 55:1963–1969
    [Google Scholar]
  24. Neilands J. B. 1982; Microbial envelope proteins related to iron. Annual Review of Microbiology 36:285–309
    [Google Scholar]
  25. Selander R. K., Korhonen T. K., Vaisanen-Rhen V., Williams P. H., Pattison P. E., Caugant D. A. 1986; Genetic relationships and clonal structure of strains of Escherichia coli causing neonatal septicemia and meningitis. Infection and Immunity 52:213–222
    [Google Scholar]
  26. Simon E. H., Tessman I. 1963; Thymidine- requiring mutants of phage T4. Proceedings of the National Academy of Sciences of the United States of America 50:526–532
    [Google Scholar]
  27. Smith H. W., Green P., Parsell Z. 1983; Vero cell toxins in Escherichia coli and related bacteria: transfer by phage and conjugation and toxic action in laboratory animals, chickens and pigs. Journal of General Microbiology 129:3121–3137
    [Google Scholar]
  28. Stenderup J., Ørskov F. 1983; The clonal nature of enteropathogenic Escherichia coli strains. Journal of Infectious Diseases 148:1019–1024
    [Google Scholar]
  29. Stevenson P., Griffiths E. 1985; Detection of antibodies and antigens by immunblotting. In The Virulence of Escherichia coli, Reviews and Methods pp 457–464 Sussman M. Edited by London: Academic Press;
    [Google Scholar]
  30. Stuart S. J., Greenwood J. K. T., Luke R. K. J. 1982; Iron-suppressible production of hydroxamate by Escherichia coli isolates. Infection and Immunity 36:870–875
    [Google Scholar]
  31. Sussman M. 1985; Esherichia coli in human and animal disease. In The Virulence of Escherichia coli; Reviews and Methods pp 7–47 Sussman M. Edited by London: Academic Press;
    [Google Scholar]
  32. Vaisanen-Rhen V., Elo J., Vaisanen E., Siitonen A., Ørskov I., Ørskov F., Svenson S. B., Makela P. H., Korhonen T. K. 1984; P-fimbriated clones among uropathogenic Escherichia coli strains. Infection and Immunity 43:149–155
    [Google Scholar]
  33. Valvano M. A., Silver R. P., Crosa J. H. 1986; Occurrence of chromosome or plasmid-mediated aerobactin iron transport systems and hemolysin production among clonal groups of human invasive strains of Escherichia coli Kl. Infection and Immunity 52:192–199
    [Google Scholar]
  34. Williams P., Chart H., Griffiths E., Stevenson P. 1987; Expression of high affinity iron uptake systems by clinical isolates of Klebsiella. FEMS Microbiology Letters 44:407–412
    [Google Scholar]
  35. Williams P. H. 1979; Novel iron uptake system specified by ColV plasmids: an important component in the virulence of Escherichia coli . Infection and Immunity 26:925–932
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-134-6-1549
Loading
/content/journal/micro/10.1099/00221287-134-6-1549
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error