1887

Abstract

Motility of C58C consisted of long straight runs, with relatively few tumbles. Speeds of up to 60 μm s and runs of up to 500 μm were recorded. The propulsive mechanism appeared to resemble that of Chemotaxis towards carbohydrates resolved four groups of sugars: chemoattractants with peaks at 10 (sucrose, glucose and fructose); 10 (maltose, lactulose and galactose); 10 (raffinose, stachyose and arabinose); and weak or non-attractants (palatinose, lactose, cellobiose and xylose). In descending order, the magnitude of the responses was as follows: sucrose ⪢ maltose > lactulose > glucose > galactose/fructose > stachyose/arabinose/raffinose. The amino acids valine and arginine were good chemoattractants with peaks at 10 , but no significant attraction was observed with alanine, cysteine, methionine or glycine. These results are indicative of a highly sensitive chemotaxis system towards sugars in C58C, and suggest a role for this process in the ecology of the organism.

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1988-06-01
2024-04-26
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References

  1. Adler J. 1973; A method for measuring chemotaxis and use of the method to determine optimum conditions for chemotaxis by Escherichia coli . Journal of General Microbiology 74:77–91
    [Google Scholar]
  2. Ames P., Schluederberg S. A., Bergman K. 1980; Behavioural mutants of Rhizobium meliloti . Journal of Bacteriology 141:722–727
    [Google Scholar]
  3. Ashby A. M., Watson M. D., Shaw C. H. 1987; A Ti-plasmid determined function is responsible for chemotaxis towards the plant wound product acetosyringone. FEMS Microbiology letters 41:189–192
    [Google Scholar]
  4. Bashan Y. 1986; Migration of the rhizosphere bacteria Azospirillum brasilenseand Pseudomonas fluorescenstowards wheat roots in the soil. Journal of General Microbiology 132:3407–3414
    [Google Scholar]
  5. Berg H. C., Brown D. A. 1972; Chemotaxis in Escherichia colianalysed by three-dimensional tracking. Nature; London: 239500–504
    [Google Scholar]
  6. Chet I., Zilberstein Y., Henis Y. 1973; Chemotaxis of Pseudomonas lachrymansto plant extracts and to water droplets collected from the leaf surfaces of resistant and susceptible plants. Physiological Plant Pathology 3:473–479
    [Google Scholar]
  7. Götz R., Schmitt R. 1987; Rhizobium melilotiswims by unidirectional, intermittent rotation of right-handed flagellar helices. Journal of Bacteriology 169:3146–3150
    [Google Scholar]
  8. Götz R., Limmer N., Ober K., Schmitt R. 1982; Motility and chemotaxis in two strains of Rhizobiumwith complex flagella. Journal of General Microbiology 128:789–798
    [Google Scholar]
  9. Kandler O., Hopf H. 1980; Occurrence, metabolism and function of oligosaccharides. In The Biochemistry of Plants 10 pp. 221–270 New York: Academic Press;
    [Google Scholar]
  10. Kerr A. 1969; Crown gall of stone fruit. I. Isolation of Agrobacterium tumefaciensand related species. Australian Journal of Biological Sciences 22:111–116
    [Google Scholar]
  11. Kerr A. 1974; Soil microbiological studies on Agrobacterium radiobacterand biological control of crown gall. Soil Science 118:168–172
    [Google Scholar]
  12. Klapwijk P. M., Van Beelen P., Schilperoort R. A. 1979; Isolation of a recombination deficient Agrobacterium tumefaciensmutant. Molecular and General Genetics 173:171–175
    [Google Scholar]
  13. Lichtenstein C. 1986; A bizarre vegetal bestiality. Nature; London: 322682–683
    [Google Scholar]
  14. Miller J. H. 1972 Experiments in Molecular Genetics Cold Spring Harbor, New York: Cold Spring Harbor Laboratory;
    [Google Scholar]
  15. Nester E. W., Gordon M. P., Amasino R. M., Yanofsky M. 1984; Crown gall: a molecular and physiological analysis. Annual Review of Plant Physiology 35:387–413
    [Google Scholar]
  16. Nikaido H., Vaara M. 1985; Molecular basis of bacterial outer membrane permeability. Microbiological Reviews 49:1–32
    [Google Scholar]
  17. Okker R. J. H., Spaink H., Hille J., Van Brussel T. A. N., Lugtenberg B., Schilperoort R. A. 1984; Plant inducible promoter of the Agrobacterium tumefaciensTi-plasmid. Nature; London: 312564–566
    [Google Scholar]
  18. Schroth M. N., Weinhold A. R., Mccain A. H., Hildebrand D. C., Ross N. 1971; Biology and control of Agrobacterium tumefaciens . Hilgardia 40:536–552
    [Google Scholar]
  19. Shaw C. H., Ashby A. M., Watson M. D. 1986; Plant tumour induction. Nature; London: 324415
    [Google Scholar]
  20. Stachel S. E., Zambryski P. 1986; virAand virGcontrol the plant-induced activation of the T-DNA transfer process of A. tumefaciens . Cell 46:325–333
    [Google Scholar]
  21. Stachel S. E., Messens E., Van Montagu M., Zambryski P. 1985; Identification of the signal molecules produced by wounded plant cells that activate T-DNA transfer in Agrobacterium tumefaciens . Nature; London: 318624–629
    [Google Scholar]
  22. Stachel S. E., Nester E. W., Zambryski P. C. 1986; A plant cell factor induces Agrobacterium tumefaciens virgene expression. Proceedings of the National Academy of Sciences of the United States of America 83:379–383
    [Google Scholar]
  23. Van Larebeke N., Engler G., Holsters M., Van Den Elsacker S., Zaenen I., Schilperoort R. A., Schell J. 1974; Large plasmid in Agrobacterium tumefaciensessential for crown gall inducing ability. Nature; London: 252:169–170
    [Google Scholar]
  24. Watson B., Currier T. C., Gordon M. P., Chilton M.-D., Nester E. W. 1975; Plasmid required for virulence of Agrobacterium tumefaciens . Journal of Bacteriology 123:255–264
    [Google Scholar]
  25. Winans S. C., Ebert P. R., Stachel S. E., Gordon M. P., Nester E. W. 1986; A gene essential for Agrobacteriumvirulence is homologous to a family of positive regulatory loci. Proceedings of the National Academy of Sciences of the United States of America 83:8278–8282
    [Google Scholar]
  26. Ziegler R. N., Peirce C., Bergman K. 1986; Mapping and cloning of a fla-cheregion of the Rhizobium melilotichromosome. Journal of Bacteriology 168:785–790
    [Google Scholar]
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