1887

Abstract

The developmental cycle of the obligate intracellular prokaryote involves the serial alternation of two distinct morphological forms of the organism. To examine the basis of chlamydial differentiation we have searched for developmentally regulated gene products in this species. Chlamydia-infected cells were pulse-labelled with [S]cysteine at various stages of development and the products of synthesis examined by SDS-PAGE. Our results indicate that the synthesis of the cysteine-rich outer-membrane proteins is developmentally regulated, occurring only late in the cycle during the conversion of reticulate bodies to elementary bodies. Both hydroxyurea and ampicillin block this conversion; as a result of this blockade the cysteine-rich outer-membrane proteins are not produced in the presence of either drug.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-134-4-997
1988-04-01
2022-01-27
Loading full text...

Full text loading...

/deliver/fulltext/micro/134/4/mic-134-4-997.html?itemId=/content/journal/micro/10.1099/00221287-134-4-997&mimeType=html&fmt=ahah

References

  1. Barbour A. J., Amano K.-I., Hackstadt I., Perry L., Caldwell H. D. 1982; Chlamydia trachomatishas penicillin-binding proteins but not detectable muramic acid. Journal of Bacteriology 151:420–428
    [Google Scholar]
  2. Batteiger B. E., Newhall W. J. V., Jones R. B. 1968; Differences in outer membrane proteins of lymphogranuloma venereum and trachoma biovars ofChlamydia trachomatis. Infection and Immunity 50:488–494
    [Google Scholar]
  3. Bavoil P., Ohlin A., Schachter J. 1984; Role of disulfide bonding in outer membrane structure and permeability inChlamydia trachomatis. Infection and Immunity 44:479–485
    [Google Scholar]
  4. Becker Y. 1978; The chlamydia: molecular biology of procaryotic obligate parasites of eucaryotes. Microbiological Reviews 42:274–306
    [Google Scholar]
  5. Bonner W. M., Laskey R. A. 1974; A film detection method for tritium-labeled proteins and nucleic acids in polyacrylamide gels. European Journal of Biochemistry 46:83–88
    [Google Scholar]
  6. Caldwell H. D., Kromhout J., Schachter J. 1981; Purification and partial characterization of the major outer membrane proteins ofChlamydia trachomatis. Infection and Immunity 31:1161–1176
    [Google Scholar]
  7. Garrett A. J., Harrison M. J., Manire G. P. 1974; A search for the bacterial mucopeptide component, muramic acid, inChlamydia. Journal of General Microbiology 80:315–318
    [Google Scholar]
  8. Hackstadt T., Todd W. J., Caldwell H. D. 1985; Disulfide-mediated interactions of the chlamydial major outer membrane protein: role in the differentiation of chlamydiae? . Journal of Bacteriology 161:25–31
    [Google Scholar]
  9. Hatch T. P., Vance D. W. Jr Al-Hossainy E. 1981; Identification of a major envelope protein inChalmydiaspp. Journal of Bacteriology 146:426–429
    [Google Scholar]
  10. Hatch T. P., Allan I., Pearce J. H. 1984; Structural and polypeptide differences between envelopes of infective and reproductive life cycle forms ofChlamydiaspp. Journal of Bacteriology 157:13–20
    [Google Scholar]
  11. Hatch T. P., Miceli M., Sublett J. E. 1986; Synthesis of disulfide-bonded outer membrane proteins during the developmental cycle ofChlamydia psittaciandChlamydia trachomatis. Journal of Bacteriology 165:379–385
    [Google Scholar]
  12. Hoch J. A. 1976; Genetics of bacterial sporulation. Advances in Genetics 18:69–99
    [Google Scholar]
  13. Kaiser D., Manoil C., Dworkin M. 1979; Myxobacteria: cell interactions, genetics, and development. Annual Review of Microbiology 33:595–639
    [Google Scholar]
  14. Kramer M. J., Gordon r. 1971; uurasiruc-tural analysis of the effects of penicillin and chlortetracycline on the development of genital tractChlamydia. Infection and Immunity 3:333–341
    [Google Scholar]
  15. Laemmli U. K., Favre M. 1973; Maturation of the head of bacteriophage T4. I. DNA packaging events. Journal of Molecular Biology 80:575–599
    [Google Scholar]
  16. Manire G. P., Tamura A. 1967; Preparation and chemical composition of the cell walls of mature infectious dense forms of meningopneumonitis organisms. Journal of Bacteriology 94:1178–1183
    [Google Scholar]
  17. Matsumoto A., Manire G. P. 1970; Electron microscopic observations on the effect of penicillin on the morphology ofChlamydia psittaci. Journal of Bacteriology 101:278–285
    [Google Scholar]
  18. Newhall W. J. V. 1987; Biosynthesis and disulfide cross-linking of outer membrane components during the growth cycle ofChlamydia trachomatis. Infection and Immunity 55:162–168
    [Google Scholar]
  19. Newhall W. J. V., Jones R. B. 1983; Disulfide- linked oligomers of the major outer membrane protein of chlamydiae. Journal of Bacteriology 154:998–1001
    [Google Scholar]
  20. Piggot P. J., Coote J. G. 1976; Genetic aspects of bacterial endospore formation. Bacteriological Reviews 40:908–952
    [Google Scholar]
  21. Rosenkranz H. S., Gutter B., Becker Y. 1973; Studies on the developmental cycle ofChlamydia trachomatis:selective inhibition by hydroxyurea. Journal of Bacteriology 115:682–690
    [Google Scholar]
  22. Salari S. H., Ward M. E. 1981; Polypeptide composition ofChlamydia trachomatis. Journal of General Microbiology 123:197–207
    [Google Scholar]
  23. Schachter J., Caldwell H. D. 1980; Chlamydiae. Annual Review of Microbiology 34:285–309
    [Google Scholar]
  24. Tamura A., Manire G. P. 1967; Preparation and chemical composition of the cell membranes of developing reticulate forms of meningopneumonitis organisms. Journal of Bacteriology 94:1184–1188
    [Google Scholar]
  25. Tamura A., Manire G. P. 1968; Effect of penicillin on the multiplication of meningopneumonitis organisms(Chlamydia psittaci). Journal of Bacteriology 96:875–880
    [Google Scholar]
  26. Treharne J. D., Day J., Yeo C. K., Jones B. R., Squires S. 1977; Mode for the mechanism of the irreversible effects of penicillin. In Nongonococcal Urethritis and Related Infections pp 213–214 Holmes K. K., Hobson D. Edited by Washington, DC: American Society for Microbiology;
    [Google Scholar]
  27. Zusman D. R. 1984; Cell-cell interactions and development inMyxococcus xanthus. Quarterly Review of Biology 59:119–138
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-134-4-997
Loading
/content/journal/micro/10.1099/00221287-134-4-997
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error