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The transciption of genes that function in N2 fixation (nif) and nitrogen assimilation (glnII) in Bradyrhizobium japonicum is coordinately induced in response to O2 limitation as well as symbiotic development. We have determined the relative steady-state mRNA levels for the nifH, nifDK and glnII transcription units in bradyrhizobial cells grown under a variety of levels of aerobiosis and in cells isolated from soybean root nodules. All three transcripts are found in cells grown in a rich medium sparged with O2 concentrations of 5 % (v/v) or less. This expression is qualitatively similar to that observed for Bradyrhizobium during symbiotic development. Potential physiological mechanisms for the coordinate control of these genes are discussed.
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