1887

Abstract

The formation of single-stranded breaks in DNA following UV irradiation is assessed in 34 mutants. By altering the SOS DNA-repair system, either by additional mutations or by using drugs affecting transcription or translation, it is shown that such single-stranded breaks require one or more DNA-damage-inducible functions. A UV-sensitive strain is characterized as carrying a Tn insertion into the gene. The absence of post-irradiation incision in this strain demonstrates that function is absolutely required for the incision stage of excision repair, and suggests that other mutants are ‘leaky’.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-134-2-403
1988-02-01
2021-10-26
Loading full text...

Full text loading...

/deliver/fulltext/micro/134/2/mic-134-2-403.html?itemId=/content/journal/micro/10.1099/00221287-134-2-403&mimeType=html&fmt=ahah

References

  1. Bichara M., Fuchs R. P. P. 1987; uvrC gene function has no specific role in repair of N-2- aminofluorene adducts. Journal of Bacteriology 169:423–426
    [Google Scholar]
  2. Blingsmo O. L., Steinum A. L., Rivedal E., Seeberg E. 1981; Cloning of the uvrC+ gene from Escherichia coli onto a plaque-forming phage vector. In Chromosome Damage and Repair pp. 303–309 Seeberg E., Kleppe K. Edited by New York: Plenum Press;
    [Google Scholar]
  3. Caron P. R., Kushner S. R., Grossman L. 1985; Involvement of helicase II (uvrD gene product) and DNA polymerase I in excision mediated by the uvrABC protein complex. Proceedings of the National Academy of Sciences of the United States of America 82:4925–4929
    [Google Scholar]
  4. Cooper P. K., Hanawalt P. C. 1972; Role of DNA polymerase I and the rec system in excision repair in Escherichia coli. Proceedings of the National Academy of Sciences of the United States of America 69:1156–1160
    [Google Scholar]
  5. Emmerson P. T., Hicksoni D., Gordonr L., Tomkinsona D. 1981; Cloning of recA+ and lexA+ and some of their mutant alleles; an investigationof their mutual interaction. In Chromosome Damage and Repair pp. 281–285 Seeberg E., Kleppe K. Edited by New York: Plenum Press;
    [Google Scholar]
  6. Feinberg A., Vogelstein B. 1984; A technique for radiolabelling DNA restriction endonuclease fragments to high specific activity. Analytical Biochemistry 137:266–267
    [Google Scholar]
  7. Forster J. W., Strike P. 1985; Organisation and control of the Escherichia coli uvrC gene. Gene 35:71–82
    [Google Scholar]
  8. Fuchs R. P. P., Seeberg E. 1984; pBR322 plasmid DNA modified with 2-acetylaminofluorene derivatives: transforming activity and in vitro strand cleavage by the Escherichia coli uvrABC endonuclease. EMBO Journal 3:757–760
    [Google Scholar]
  9. Granger-Schnaar M., Schnaar M., Van Sluis C. A. 1985; In vitro study of the interaction of the LexA repressor and the UvrC protein with a uvrC regulatory region. FEBS Letters 198:61–65
    [Google Scholar]
  10. Halling S. M., Simons R. W., Way J. C., Walsh R. B., Kleckner N. 1982; DNA sequence organization of Tn10’s IS10-Right and comparison with IS10-Left. Proceedings of the National Academy of Sciences of the United States of America 79:2608–2612
    [Google Scholar]
  11. Hillen W., Schollmeier K. 1983; Nucleotide sequence of the Tn10 encoded tetracycline resistance gene. Nucleic Acids Research 22:525–539
    [Google Scholar]
  12. Husain I., Van Houten B., Thomas D. C., Abdel-Morem M., Sancar A. 1985; Effect of DNA polymerase I and DNA helicase II on the turnover of UvrABC excision nuclease. Proceedings of the National Academy of Sciences of the United States of America 82:6774–6778
    [Google Scholar]
  13. Kacinski B. M., Rupp W. D. 1981; Escherichia coli uvrB protein binds to DNA in the presence of the uvrA protein. Nature; London: 294480–481
    [Google Scholar]
  14. Kato T. 1972; Excision repair characteristics of recB res and uvrC strains of Escherichia coli. Journal of Bacteriology 112:1237–1246
    [Google Scholar]
  15. Kleckner N., Barker D., Ross D., Botstein D. 1978a; Properties of the translocatable tetracycline resistance element Tn10 in Escherichia coli and bacteriophage λ. Genetics 90:427–450
    [Google Scholar]
  16. Kleckner N., Swan J. A., Zabeau M. 1978b; Restriction enzyme analysis of Tn10 insertions in the immunity region of bacteriophage lambda. Genetics 90:450–461
    [Google Scholar]
  17. Kleckner N., Reichardt K., Botstein D. 1979; Inversions and deletions of the Salmonella chromosome generated by the translocatable tetracycline resistance element Tn10. Journal of Molecular Biology 127:89–115
    [Google Scholar]
  18. Little J. W. 1980; Isolation of recombinant plasmids and phage carrying the lexA gene of Escherichia coli. Gene 10:237–247
    [Google Scholar]
  19. Little J. W., Hanawalt P. C. 1977; Induction of protein X in Escherichia coli. Molecular and General Genetics 150:237–248
    [Google Scholar]
  20. Little J. W., Edmiston S. H., Pacelli L. Z., Mount D. W. 1980; Cleavage of the Escherichia coli lexA protein by the recA protease. Proceedings of the National Academy of Sciences of the United States of America 76:6147–6151
    [Google Scholar]
  21. Maniatis T., Fritsch E. F., Sambrook J. 1982 Molecular cloning : a Laboratory Manual Cold Spring Harbor: Cold Spring Harbor Laboratory;
    [Google Scholar]
  22. Moolenaar G. F., Van Sluis C. A., Backendorf C., Van De Putte P. 1987; Regulation of the Escherichia coli excision repair gene uvrC. Overlap between the uvrC structural gene and the region coding for a 24 kD protein 15:4273–4289
    [Google Scholar]
  23. Morse L. S., Pauling C. 1975; Induction of errorprone repair as a consequence of DNA ligase deficiency in Escherichia coli. Proceedings of the National Academy of Sciences of the United States of America 72:4645–4649
    [Google Scholar]
  24. Pacelli L. Z., Edmiston S. H., Mount D. W. 1979; Isolation and characterization of amber mutations in the lex A gene of Escherichia coli K-12. Journal of Bacteriology 137:568–573
    [Google Scholar]
  25. Roberts R. J., Strike P. 1981; Efficiency of Escherichia coli repair processes on UV-damaged transforming plasmid DNA. Plasmid 5:213–220
    [Google Scholar]
  26. Rupp W. D., Wilde D. E., Reno D. L., Howard-Flanders P. 1971; Exchanges between DNA strands in UV-irradiated Escherichia coli. Journal of Molecular Biology 61:25–44
    [Google Scholar]
  27. Sancar A., Rupp W. D. 1983; A novel repair enzyme: UVRABC excision nuclease cuts a DNA strand on both sides of the damaged region. Cell 33:249–260
    [Google Scholar]
  28. Satta G., Gudas L. J., Pardee A. B. 1979; Degradation of Escherichia coli DNA: evidence for limitation in vivo by protein X, the recA gene product. Molecular and General Genetics 168:69–80
    [Google Scholar]
  29. Seeberg E., Rupp W. D. 1975; Effects of mutations in lig and polA on UV-induced strand cutting in a uvrC mutant of Escherichia coli. In Molecular Mechanisms in DNA Repair pp. 439–441 Hanawalt P. C., Setlow R. B. Edited by New York: Plenum Press;
    [Google Scholar]
  30. Seeberg E., Steinum A. L. 1982; Purification and properties of the uvrA protein from Escherichia coli. Proceedings of the National Academy of Sciences of the United States of America 79:988–992
    [Google Scholar]
  31. Seeberg E., Strike P. 1976; Excision repair of ultraviolet-irradiated deoxyribonucleic acid in plasmolysed cells of Escherichia coli. Journal of Bacteriology 125:787–795
    [Google Scholar]
  32. Seeberg E., Nissen-Meyer J., Strike P. 1976; Incision of ultraviolet-irradiated DNA by extracts of Escherichia coli requires three different gene products. Nature; London: 263524–526
    [Google Scholar]
  33. Seeberg E., Rupp W. D., Strike P. 1980; Impaired incision of ultraviolet-irradiated deoxyribonucleic acid in uvrC mutants of Escherichia coli. Journal of Bacteriology 144:97–104
    [Google Scholar]
  34. Sharma S., Moses R. E. 1979; uvrC gene function in excision repair in toluene-treated Escherichia coli. Journal of Bacteriology 137:397–408
    [Google Scholar]
  35. Sharma S., Stark T. F., Beattie W. G., Moses R. E. 1986; Multiple control elements for the uvrC gene unit of Escherichia coli. Nucleic Acids Research 14:2301–2318
    [Google Scholar]
  36. Van Sluis C. A., Moolenaar G. F., Backendorf C. 1983; Regulation of the uvrC gene of Escherichia coli K12: localization and characterization of a damage-inducible promoter. The EMBO Journal 2:2313–2318
    [Google Scholar]
  37. Southern E. 1975; Detection of specific sequences among DNA fragments separated by gel electrophoresis. Journal of Molecular Biology 98:503–517
    [Google Scholar]
  38. Strike P., Roberts R. J. 1982; Resident enhanced repair: novel repair process action on plasmid DNA transformed into Escherichia coli K-12. Journal of Bacteriology 150:385–388
    [Google Scholar]
  39. Strike P., Rupp W. D. 1985; Cross-linking studies with the uvrA and uvrB proteins of Escherichia coli. Mutation Research 145:43–48
    [Google Scholar]
  40. Tang M. S., Ross L. 1985; Single-strand breakage of DNA in UV-irradiated uvrA, uvrB, and uvrC mutants of Escherichia coli. Journal of Bacteriology 161:933–938
    [Google Scholar]
  41. Wilson G. G., Young K. K. Y., Edlin G. J., Konigsberg W. 1979; High frequency generalised transduction by bacteriophage T4. Nature; London: 28080–82
    [Google Scholar]
  42. Yeung A. T., Mattes W. B., Grossman L. 1986; Protein complexes formed during the incision reaction catalysed by the Escherichia coli UvrABC endonuclease. Nucleic Acids Research 14:2567–2582
    [Google Scholar]
  43. Yoakum G. H., Grossman L. 1981; Identification of the uvrC protein. Nature; London: 292171–173
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-134-2-403
Loading
/content/journal/micro/10.1099/00221287-134-2-403
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error