1887

Abstract

When eight strains of the oleaginous yeasts and were starved of carbon after having accumulated lipid up to 34% of their biomass, the lipid was readily converted to new biomass in all cases except the two strains of When D was grown in a two-stage chemostat with the second stage as a carbon-starvation vessel (but containing NH ) biosynthesis of new biomass reached 1·9 ± 0·2 g per g lipid utilized. Experiments in a single-stage chemostat undergoing transition from lipid accumulation (nitrogen-limited medium) to carbon-starvation conditions showed that the lipid in was rapidly mobilized. When the lipid was pre-labelled with C, a transitory pool of rapidly metabolizable non-lipid material appeared within 1.5 h of the initiation of starvation. Rates of lipid loss indicated that initiation of lipid degradation occurred immediately carbon was lost from the external medium.

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/content/journal/micro/10.1099/00221287-134-2-339
1988-02-01
2021-10-23
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