1887

Abstract

The electron transfer system that transports electrons from formate to nitrogenase was examined in extracts of the obligate methanotroph OB3b. By supplementing a crude nitrogenase extract with NAD, formate dehydrogenase, NADH-ferredoxin reductase and FMN (an FDH cofactor), an electron transport system was established that coupled formate oxidation to nitrogenase-dependent acetylene reduction. The ferredoxin dependence of this reaction was demonstrated by its severe inhibition by antibodies to ferredoxin. The reaction sequence is as follows: formate → formate dehydrogenase → NADH → NADH-ferredoxin reductase → ferredoxin → nitrogenase.

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1988-12-01
2021-08-04
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