SUMMARY: A chemically defined medium for growing mass cultures of was developed. The growth characteristics and the effects of variations in the culture conditions and medium constituents were studied. The results show that can be grown in a completely defined medium with rates and yields nearly comparable to those obtained in non-defined media. In the defined medium, K but not Na, was indispensable for growth; unlike in media containing proteose peptone, the growth rate was independent of the presence of insoluble Fe complexes, but was more dependent on culture temperature. Although has an absolute requirement for preformed purine and pyrimidine bases in addition to several amino acids, its proliferation and growth were primarily under amino acid control.


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