SUMMARY: The structural gene of the locus of was replaced with the promoterless gene of . The :: gene fusion was transferred to the chromosome and β-galactosidase activity was measured under sporulation conditions. Expression of the hybrid gene could be detected as early as 40 min after the induction of sporulation. Transcription of the :: gene was dependent on the products of two stage 0 loci, and . Mutations in the and loci did not impede expression of and, therefore, neither of the sigma factors coded for by these loci seems to be necessary for its transcription. Consequently, the locus does not seem to be part of the dependent sequence of operons involved in the developmental change, although its protein product is clearly needed for the completion of spore formation.


Article metrics loading...

Loading full text...

Full text loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error