Summary: Low levels of invertase (EC activity were observed in most diploid strains of used in this work. There was no effect of mating type on invertase levels, and cell surface was not a limiting factor, because an increase in ploidy did not cause further decrease in specific invertase activity. Finally, some diploids showed the activity expected from the additive effects of different genes, and haploid strains possessing two genes expressed very variable invertase activities depending on the strain. This suggested the existence of one or more additional genes which control the levels of invertase. Genetic analysis of strains provided evidence of the existence of a new gene, which drastically reduced the specific invertase activity in strains possessing active alleles. The recessive allele of this gene () allows expression of higher levels of invertase. We suggest that genes similar to are responsible for the low levels of invertase activity observed in diploid strains of .


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