SUMMARY: A3(2) and 66 strains were shown to be sensitive to the galactose analogue 2-deoxy--galactose. Spontaneous resistant mutants were isolated that were Gal and lacked the enzyme galactokinase. The gene (structural gene for galactokinase) from was cloned into using the low copy number vector pIJ922. The resulting plasmid (pMT650), which contained a 14 kb insert, complemented mutations in both species. The presence of the gene on a 2·8 kb RI fragment was confirmed by expressing it in where it complemented a well characterized mutation.


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