1887

Abstract

The antibiotic moenomycin is a valuable biochemical tool for studying the metabolism of peptidoglycan and the autolytic system in , since as a specific inhibitor of peptidoglycan polymerases it can efficiently promote cell lysis. In liquid media the bacteriolytic effect on K12 was dependent on the concentration of moenomycin, on growth phase and on growth rate. Before lysis cells underwent major morphological alterations. In sucrose-containing medium complete transformation to osmotically sensitive spheroplasts was easily achieved by addition of moenomycin. The minimum inhibitory concentration of the antibiotic varied with the strain of and was highly dependent on the growth medium. A tritiated derivative of moenomycin, [H]decahydromoenomycin A, was prepared and found to have the same inhibiting efficiency. Its binding to membranes and membrane proteins was investigated. The absence of irreversible binding suggested that moenomycin might be a competitive inhibitor of the peptidoglycan polymerases. Spontaneous moenomycin resistant variants were isolated at a frequency of about 10.

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1987-03-01
2024-12-06
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References

  1. Broome-Smith J.K., Edelman A., Yousif S., Spratt B.G. 1985; The nucleotide sequences of the pon A and pon B genes encoding penicillinbinding proteins 1A and 1B of Escherichia coli K 12. European Journal of Biochemistry 147:437–446
    [Google Scholar]
  2. Chen P.S., Toribara T.Y., Warner H. 1956; Microdetermination of phosphorus. Analytical Chemistry 28:1756–1758
    [Google Scholar]
  3. Clarke L., Carbon J. 1976; A colony bank containing synthetic Col El hybrid plasmids representative of the entire E. coli genome. Cell 9:91–99
    [Google Scholar]
  4. Gale E.F., Cundliffe E., Reynolds P.E., Richmond M.H., Waring M.J. 1980; The molecular basis of antibiotic action. London:: John Wiley.;
    [Google Scholar]
  5. Goodell E.W. 1985; Recycling of murein by Escherichia coli.. Journal of Bacteriology 163:305–310
    [Google Scholar]
  6. Goodell E.W., Schwartz U. 1983; Cleavage and resynthesis of peptide cross bridges in Escherichia coli murein. Journal of Bacteriology 156:136–140
    [Google Scholar]
  7. VanHeijenoort Y., Derrien M., VanHeijenoort J. 1979; Polymerization by transglycosylation in the biosynthesis of the peptidoglycan of Escherichia coli K 12 and its inhibition by antibiotics. FEBS Letters 99:141–144
    [Google Scholar]
  8. VanHeijenoort Y., VanHeijenoort J. 1980; Biosynthesis of the peptidoglycan of Escherichia coli K 12. Properties of the in vitro polymerization by transglycosylation. FEBS tetters 110:241–244
    [Google Scholar]
  9. Huber G. 1979; Moenomycin and related phosphorus containing antibiotics. In Antibiotic V/I. Mechanism of Action of Antibacterial Agents, pp Hahn F. E. Edited by Berlin & Heidelberg: Springer-Verlag;
    [Google Scholar]
  10. Ishino F., Matsuhashi M. 1981; Peptidoglycan synthetic enzyme activities of highly purified penicillin-binding protein 3 in Escherichia coli : a septumforming reaction sequence. Biochemical and Biophysical Research Communications 101:905–911
    [Google Scholar]
  11. Kato J., Suzuki H., Hirota Y. 1984; Overlapping of the coding regions for α and γ components of penicillin-binding protein lb in Escherichia coli.. Molecular and General Genetics 196:449–457
    [Google Scholar]
  12. Leduc M., Kasra R., vanHeijenoort J. 1982; Induction and control of the autolytic system of Escherichia coli.. Journal of Bacteriology 152:26–34
    [Google Scholar]
  13. Leduc M., Frehel C., vanHeijenoort J. 1985; Correlation between degradation and ultrastructure of peptidoglycan during autolysis of Escherichia coli.. Journal of Bacteriology 161:627–635
    [Google Scholar]
  14. Linnett P.E., Strominger J.L. 1973; Additional antibiotic inhibitors of peptidoglycan synthesis. Antimicrobial Agents and Chemotherapy 4:231–236
    [Google Scholar]
  15. Matsuhashi M., Ishino F., Nakagawa J., Mitsui K., Nakajima-Iijima S., Tamaki S. 1981; Enzymatic activities of penicillin-binding proteins of Escherichia coli and their sensitivities to β-lactam antibiotics. In β-Lactam Antibiotics, pp 169–184 Salton M., Shockman G. D. Edited by New York: Academic Press;
    [Google Scholar]
  16. Nakagawa J., Tamaki S., Tomioka S., Matsuhashi M. 1984; Functional biosynthesis of cell wall peptidoglycan by polymorphic bifunctional polypeptides. Penicillin-binding protein IBs of Escherichia coli with activities of transglycosylase and transpeptidase. Journal of Biological Chemistry 259:13937–13946
    [Google Scholar]
  17. Nikaido H. 1979; Non specific transport through the outer membrane. In Bacterial Outer Membrane, pp 360–407 Inouye M. Edited by New York: John Wiley;
    [Google Scholar]
  18. Rogers H.J., Perkins H.R., Ward J.B. 1980 Microbial Cell Walls and Membranes. London:: Chapman and Hall.;
    [Google Scholar]
  19. Slusarchyk W.A. 1971; Chemical and biological aspects of a new family of phosphorus containing antibiotics. Biotechnology and Bioengineering 13:399–407
    [Google Scholar]
  20. Spratt B.G. 1977; Properties of the penicillinbinding protein of Escherichia coli.. European Journal of Biochemistry 72:341–352
    [Google Scholar]
  21. Suzuki H., vanHeijenoort Y., Tamura T., Mizoguchi J., Hirota Y., Vanheijenoort J. 1980; In vitro peptidoglycan polymerization catalysed by penicillin-binding protein 1 b of Escherichia coli K 12. FEBS Letters 110:245–249
    [Google Scholar]
  22. Takahashi S., Serita K., Arai M. 1983; Structure of pholipomycin. Tetrahedron Letters 24:499–502
    [Google Scholar]
  23. Tamura T., Suzuki H., Nishimura Y., Mizoguchi J., Hirota Y. 1980; On the process of cellular division in Escherichia coli: isolation and characterization of penicillin-binding proteins 1a, 1b and 3. Proceedings of the National Academy of Sciences of the United States of America 77:4499–4503
    [Google Scholar]
  24. Wasielewski E.V., Muschaweck R., Schütze E. 1965; Moenomycin, a new antiobiotic. III. Biological properties. Antimicrobial Agents and Chemotherapy743–748
    [Google Scholar]
  25. Weidel W., Pelzer H. 1964; Bagshaped macromolecules. A new outlook on bacterial cell walls. Advances in Enzymology 26:193–232
    [Google Scholar]
  26. Welzel P., Witteler F.J., Müller D., Riemer W. 1981; Structure of the antiobiotic moenomycin A. Angewandte Chemie (International Edition in English) 20:121–123
    [Google Scholar]
  27. Welzel P., Wietfeld B., Kunisch F., Schubert T., Hobert K., Duddeck H., Müller D., Huber G., Maggio J.E., Williams D.H. 1983; Moenomycin A: further structural studies and preparation of simple derivatives. Tetrahedron 39:1583–1591
    [Google Scholar]
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