SUMMARY: Bacteriophage cloning vector ϕ105J27, the construction of which is described in an accompanying paper, has been used for shotgun cloning of sporulation genes in . Various genomic libraries have been constructed and screened for the presence of recombinant phages capable of transducing strains containing sporulation () mutations to Spo. Of a total of 30 loci tested, transducing phages have been isolated for 23, more than half of the known loci. Included are nine loci () that do not appear to have been cloned previously. Preliminary genetic characterization of some of the new clones by a rapid screening procedure has enabled the status of various sporulation loci to be clarified.


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