1887

Abstract

Exopolysaccharides (EXPs) of are associated with virulence in animal models. An EXP from the strain Smith diffuse was previously detected in 64·3 % of clinical isolates. EXP was isolated from culture supernatants of this strain after DNAase, RNAase, phosphodiesterase I and lysostaphin treatment, and was further purified by cation-exchange and molecular-sieve chromatography. Isoelectric focusing revealed a pl of 3·6 for the EXP while the pl of teichoic acid was < 2·7. Crossed immunoelectrophoresis with homologous Smith diffuse antisera indicated that the EXP contained two immunological components. A major precipitin line persisted after the antisera had been absorbed with the non-EXP-producing variant strain, Smith compact, while the second component was removed. Tandem immunoelectrophoresis also demonstrated that the EXP was distinct from teichoic acid. The EXP contained 2-amino-2-deoxyglucuronic acid, glucose, mannose and galactose. No fatty acids or nucleic acids were present and total protein content was < 2%. Teichoic acid could not be demonstrated in the EXP, thus further substantiating the immunological studies. EXP isolated by the present method can be used for further serological and virulence studies.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-133-2-431
1987-02-01
2021-07-30
Loading full text...

Full text loading...

/deliver/fulltext/micro/133/2/mic-133-2-431.html?itemId=/content/journal/micro/10.1099/00221287-133-2-431&mimeType=html&fmt=ahah

References

  1. Amador E., Urban J. 1972; Simplified serum phosphorus analyses by continuous-flow ultraviolet spectrophotometry. Clinical Chemistry 18:601–604
    [Google Scholar]
  2. Brock J. H., Reiter B. 1976; Chemical and biological properties of extracellular slime produced by Staphylococcus aureus grown in high-carbohydrate, high-salt medium. Infection and Immunity 13:653–660
    [Google Scholar]
  3. Caputy G. G., Costerton J. W. 1984; Immunological examination of the glycocalyces of the Staphylococcus aureus strain Wiley and Smith. Current Microbiology 11:297–302
    [Google Scholar]
  4. Clamp J. R., Bhatti T., Chambers R. E. 1971; The determination of carbohydrate in biological materials by gas-liquid chromatography. In Methods of Biochemical Analysis 1 pp. 229–344 Glick D. Edited by New York: Wiley;
    [Google Scholar]
  5. Daly J. A., Ertingshausen G. 1972; Direct method for determining inorganic phosphate in serum with the CentrifiChem’. Clinical Chemistry 18:262–265
    [Google Scholar]
  6. Ekstedt R. D., Bernhard J. M. 1973; Preparation and characterization of a slime layer material produced by Staphylococcus aureus (36964). Proceedings of the Society of Experimental Biology and Medicine 142:86–91
    [Google Scholar]
  7. Fournier J. M., Vann W. F., Karakawa W. W. 1984; Purification and characterization of Staphylococcus aureus type 8 capsular polysaccharide. Infection and Immunity 45:87–93
    [Google Scholar]
  8. Frazer B. A., Mallette M. F. 1973; An improved isolation method and new composition data for Forssman hapten from sheep erythrocytes. Immunochemistry 10:745–753
    [Google Scholar]
  9. Hanessian S., Haskell T. H. 1964; Structural studies on staphylococcal polysaccharide antigen. Journal of Biological Chemistry 239:2758–2764
    [Google Scholar]
  10. Haskell T. H., Hanessian S. 1963; 2-Amino-2- deoxy-d-glucuronic acid, the main constituent of SPA (staphylococcal polysaccharide antigen). Nature; London: 1991075–1076
    [Google Scholar]
  11. Haskell T. H., Hanessian S. 1964; The purification and characterization of a new active immunizing polysaccharide prepared from Staphylococcus aureus . Biochimica et biophysica acta 83:35–41
    [Google Scholar]
  12. Haukenes G. 1962; Immunochemical studies on polysaccharide A of Staphylococcus aureus. 1. On the chemical basis of the serological reactivity and the nature of the extraction process. Acta pathologica et microbiologica scandinavica 55:463–474
    [Google Scholar]
  13. Hunt G. A., Moses A. J. 1958; Acute infection of mice with Smith strain of Staphylococcus aureus . Science 128:1574–1575
    [Google Scholar]
  14. Koenig M. G. 1962; Factors relating to the virulence of staphylococci. I. Comparative studies on two colonial variants. Yale Journal of Biology and Medicine 34:537–559
    [Google Scholar]
  15. Kozel T. R., Gotschlich E. C. 1982; The capsule of Cryptococcus neoformans passively inhibits phagocytosis of the yeast by macrophages. Journal of Immunology 129:1675–1680
    [Google Scholar]
  16. Lambert M. A., Moss C. W. 1983; Comparison of the effects of acid and base hydrolyses on hydroxy and cyclopropane fatty acids in bacteria. Journal of Clinical Microbiology 18:1370–1377
    [Google Scholar]
  17. Liau D., Melly M. A., Hash J. H. 1974; Surface polysaccharide from Staphylococcus aureus M that contains taurine, d-aminogalacturonic acid, and d-fucosamine. Journal of Bacteriology 119:913–922
    [Google Scholar]
  18. Mcdonald H. C., Karakawa W. W. 1970; Immunochemical analysis of a uronic acid polymer of Staphylococcus epidermidis, strain 53. Journal of Immunology 105:389–395
    [Google Scholar]
  19. Metcalfe L. D., Schmitz A. A. 1961; The rapid preparation of fatty acid esters for gas chromatographic analysis. Analytical Chemistry 33:363–364
    [Google Scholar]
  20. Morse S. I. 1962; Isolation of a surface antigen of Staphylococcus aureus . Journal of Experimental Medicine 115:295–311
    [Google Scholar]
  21. Murthy S.V.K.N., Melly M. A., Harris T. M., Hellerqvist C. G., Hash J. H. 1983; The repeating sequence of the capsular polysaccharide of Staphylococcus aureus M. Carbohydrate Research 117:113–123
    [Google Scholar]
  22. Oeding P. 1957; Agglutinability of pyogenic staphylococci at various conditions. Acta pathologica et microbiologica scandinavica 41:310–324
    [Google Scholar]
  23. Peterson P. K., Wilkinson B. J., Kim Y., Schmel-Ing D., Douglas S. D., Quie P. G., Verhoef J. 1978; The key role of peptidoglycan in opsonization of Staphylococcus aureus . Journal of Clinical Investigation 61:597–609
    [Google Scholar]
  24. Righetti P. G., Drysdale J. W. 1979; Isoelectric focusing. In Laboratory Techniques in Biochemistry and Molecular Biology pp. 1–323 Work T. S., Work E. Edited by New York: North-Holland Publishing Co;
    [Google Scholar]
  25. Smith J. M., Dubos R. J. 1956; The behavior of virulent and avirulent staphylococci in the tissues of normal mice. Journal of Experimental Medicine 103:87–107
    [Google Scholar]
  26. Taylor R. L., Conrad H. E. 1972; Stoichiometric depolymerization of polyuronides and glycos- aminoglycuronans to monosaccharides following reduction of their carbodiimide-activated carboxyl groups. Biochemistry 11:1383–1388
    [Google Scholar]
  27. Verbrugh H. A., Peters R., Rozenberg-Arska M., Peterson P. K., Verhoef J. 1981; Antibodies to cell wall peptidoglycan of Staphylococcus aureus in patients with serious staphylococcal infections. Journal of Infectious Diseases 144:1–9
    [Google Scholar]
  28. West T. E., West M. E., Mylotte J. M. 1985; Antiserum agar method for identification of Smith type exopolysaccharides in clinical isolates of Staphylococcus aureus . Journal of Clinical Microbiology 21:490–492
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-133-2-431
Loading
/content/journal/micro/10.1099/00221287-133-2-431
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error