Procedures are described for the selection of mutants that constitutively take up and phosphorylate fructose, and convert it to fructose 1,6-bisphosphate. The phenotype of such mutants is described. The altered regulatory gene, , is highly co-transducible with and other markers located at min 2 on the genome. In merozygotes, is dominant to . Mutants can be readily isolated that are at 42 °C but at 30 °C; moreover, the integration of a Tn transposon in the genome at min 2converts strains to . It is therefore likely that the regulatory gene specifies a repressor protein.


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