Leucine and histidine biosynthetic genes from HP1 that complemented auxotrophic mutations in TK54 were cloned in pIJ61. DNA from one leucine recombinant plasmid was subcloned into pBR322. From the latter, a recombinant plasmid was obtained that complemented the mutation in CV512 but not other leucine markers in Analysis of this and several subclones, including mutant plasmids constructed , established that the cloned gene was expressed in from the tetracycline promoter of pBR322 to produce a polypeptide of 67 kDa; the corresponding coding region was shown to be within a 1.7 kbp DNA fragment. Blot hybridization revealed corresponding homologous genes in several other streptomycetes.


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