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Summary: Δψ-reduced amiA mutants of Streptococcus pneumoniae were shown to be resistant to the positively charged antitumoral drugs 2-N-methylellipticinium (NME) and 2-N-methyl-9-hydroxyellipticinium (NMHE). Conversely, mutants selected for their resistance to NMHE were mapped within the amiA locus and exhibited the pleiotropic AmiA− phenotype. This shows that Δψ is a critical parameter in determining resistance to these drugs in S. pneumoniae and suggests that they are accumulated within this bacterium in response to Δψ. As a consequence NME and NMHE appear to be valuable tools for selecting Δψ-reduced mutants in S. pneumoniae.
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