1887

Abstract

Summary: Chromosomal DNA from the Nine Mile phase I strain of (CB9MIC7) was cloned into the cosmid vector pHC79. The resulting gene library was probed with a radiolabelled III fragment present in the parental strain but absent from a spontaneously derived Nine Mile phase II strain (CB9MIIC4). The insert, which includes the missing III fragment, was 38.5 kb in length. When DNA from this cosmid clone was hybridized to genomic DNA of the parental CB9MIC7 and its derivative CB9MIIC4, a number of fragments were missing or altered in the latter strain. Restriction mapping localized the fragments to a contiguous portion of the chromosomal DNA fragment. The data were consistent with an 18 kb deletion in the chromosome of CB9MIIC4. Another intrastrain spontaneous derivative, CB9MI514, also lacked the sentinel III fragment and carried a deletion of approximately 29 kb within the same cloned insert. Both deletions appeared to share a common terminus, within the limits of resolution. In all other strains investigated, both phase I and phase II, the DNA represented by the insert seemed intact. The strains examined were representative of various stages of phase variation. The relationship between the observed deletions and the mechanism of phase transition in Nine Mile strains is discussed.

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1986-09-01
2021-07-26
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References

  1. Amano K.-I., Williams J. C. 1984; Chemical and immunological characterization of lipopolysaccharides from phase I and phase II Coxiella burnetii. Journal of Bacteriology 160:994–1002
    [Google Scholar]
  2. Burton P. R., Stueckemann J., Welsh R. M., Paretsky D. 1978; Some ultrastructural effects of persistent infections by the rickettsia Coxiella burnetii in mouse L cells and green monkey kidney (Vero) cells. Infection and Immunity 21:556–566
    [Google Scholar]
  3. Collins J., Hohn B. 1978; Cosmids: a type of plasmid gene-cloning vector that is packageable in vitro in bacteriophage λ heads. Proceedings of the National Academy of Sciences of the United States of America 15:4242–4246
    [Google Scholar]
  4. Damrow T. A., Williams J. C., Waag D. M. 1985; Suppression of in vitro lymphocyte proliferation in C57BL/ 10 ScN mice vaccinated with phase I Coxiella burnetii. Infection and Immunity 47:149–156
    [Google Scholar]
  5. Goldman R. c., Leive L. 1980; Heterogeneity of antigens side chain length in lipopolysaccharide from Escherichia coli 01II and Salmonella typhimurium LT2. European Journal of Biochemistry 107:145–153
    [Google Scholar]
  6. Grunstein M., Hoogness D. 1975; Colony hybridization: a method for the isolation of cloned DNAs that contain a specific gene. Proceedings of the National Academy of Sciences of the United States of America 72:3691–3695
    [Google Scholar]
  7. Hackstadt T., Peacock M. G., Hitchcock P. J., Cole R. L. 1985; Lipopolysaccharide variation in Coxiella burnetii: intrastrain heterogeneity in structure and antigenicity. Infection and Immunity 48:359–365
    [Google Scholar]
  8. Johnson J. W., Eddy G. A., Pedersen C. E. Jr 1976; Biological properties of the M-44 strain of Coxiella burnetii. Journal of Infectious Diseases 133:334–338
    [Google Scholar]
  9. Kazar J., Brezina R., Schramek S., Urvolgyi J., Pospisil v., Kovacova E. 1974; Virulence, antigenics properties and physico-chemical characteristics of Coxiella bumetii strains with different chick embryo yolk sac passage history. Acta virologica 18:434–442
    [Google Scholar]
  10. Myers W. F., Baca O. G., Weman C. L. 1980; Genome size of the rickettsia Coxiella burnetii. Journal of Bacteriology 144:460–461
    [Google Scholar]
  11. O’Rourke A. T., Peacock M., Samuel J. E., Frazier M. E., Natvig D. O., Mallavia L. P., Baca O. 1985; Genomic analysis of phase I and II Coxiella burnetii with restriction endonucleases. Journal of General Microbiology 131:1543–1546
    [Google Scholar]
  12. Rigby P. W. J., Dieckmann M., Rhodes C., Berg P. 1977; Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I. Journal of Molecular Biology 113:237–251
    [Google Scholar]
  13. Samuel J. E., Frazier M. E., Mallavia L. P. 1985; Correlation of plasmid type and disease caused by Coxiella burnetii. Infection and Immunity 49:775–779
    [Google Scholar]
  14. Schramek S., Mayer H. 1982; Different sugar compositions of lipopolysaccharides isolated from phase I and phase II cells of Coxiella burnetii. Infection and Immunity 38:53–57
    [Google Scholar]
  15. Stoker M. G. P., Fisbt P. 1956; Phase variation of the Nine Mile and other strains of Rickettsia burnetii. Canadian Journal of Microbiology 2:310–321
    [Google Scholar]
  16. Vodkin M. H., Williams J. C., Stephenson E. H. 1986; Genetic heterogeneity among isolates of Coxiella burnetii. Journal of General Microbiology 132:455–463
    [Google Scholar]
  17. Williams B. G., Blattner F. R. 1980; Bacteriophage λ vectors for DNA cloning. Genetic Engineering 2:201 Edited by Setlow J. K., Hollaender A. New York: Plenum Press;
    [Google Scholar]
  18. Williams J. C., Cantjlbll J. L. 1982; Biological and immunological properties of Coxiella burnetii vaccines in C57BL/10 ScN endotoxin-nonresponder mice. Infection and Immunity 35:1091–1102
    [Google Scholar]
  19. Williams J. C., Stewart S. 1984; Identification of immunogenic proteins of Coxiella burnetii phase variants. Microbiology pp 257–262 Edited by Schlessinger D. Washington, DC: American Society for Microbiology;
    [Google Scholar]
  20. William J. C., Peacock M. G., McCaul T. F. 1981; Immunological and biological characterization of Coxiella burnetii, phase I and II, separated from host components. Infection and Immunity 32:84–851
    [Google Scholar]
  21. Williams J. C., Johnston M. R., Peacock M. G., Thomas L. A., Stewart S., Polltis J. L. 1984; Monoclonal antibodies distinguish phase variants of Coxiella burnetii. Infection and Immunity 43:421–428
    [Google Scholar]
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