1887

Abstract

Summary: pFM739, an R plasmid from that encodes penicillin, streptomycin and sulphonamide resistance, and the enterobacterial IncQ(P-4) plasmid RSF1010, which encodes streptomycin and sulphonamide resistance, were incompatible, and were mobilized by the same conjugative plasmids. Restriction mapping confirmed a high degree of similarity between both R plasmids; pFM739 carried DNA fragments corresponding to the known replication and resistance regions of RSF1010. pFM739 also carried an extra segment with the same restriction map as that described for the β-lactamase-coding region of transposon Tn It is suggested that the R plasmids isolated from commensal sp. could have resulted from transposition of a Tn -like genetic element to an RSF1010-like plasmid, and that they contain deletion derivatives of transposon Tn

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1986-09-01
2022-01-20
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References

  1. Albriiton W. L., Brunton J. L., Slaney L., McLean I. 1982; Plasmid-mediated sulfonamide resistance in Haemophilus ducreyi. Antimicrobial Agents and Chemotherapy 21:159–165
    [Google Scholar]
  2. Bagdasarian M., Lurz R., Ruckert B., Franklin F. C. H., Bagasarian M. M., Frey J., Timmis K. N. 1981; Specific-purpose plasmid cloning vectors II. Broad host range, high copy number, RSF 1010-derived vectors, and a host vector system for gene cloning in Pseudomonas. Gene 16:237–247
    [Google Scholar]
  3. Barth P. T., Tobin L., Sharpe G. S. 1981; Development of broad host-range plasmid vectors. In Molecular Biology, Pathogenicity and Ecology of Bacterial Plasmids pp. 439–448 Edited by Levy S. B., Clowes R. C., Koenig E. L. New York: Plenum Press;
    [Google Scholar]
  4. Cenamor R., Rotger R., Nombela C. 1986; Characterization of β-lactamases produced by commensal Neisseria sicca and Branhamella catarrhalis. Microbiología 2:29–36
    [Google Scholar]
  5. Cohen S. N., Chang A. c. Y., Hsu L. 1972; Non-chromosomal antibiotic resistance in bacteria: genetic transformation of Escherichia coli by R-factor DNA. Proceedings of the National Academy of Sciences of the United States of America 69:2110–2114
    [Google Scholar]
  6. Dillon J. R., Pauze M., Yeung K.-H. 1983; Spread of penicillinase-producing and transfer plasmids from the gonococcus to Neisseria meningitidis. Lancet 1:779–781
    [Google Scholar]
  7. Fayet O., Froment Y., Piffaretti J. C. 1982; β-Lactamase-specifying plasmids isolated from Neisseria gonorrhoeae have retained an intact right part of a Tn3-like transposon. Journal of Bacteriology 149:136–144
    [Google Scholar]
  8. Genco C. A., Knapp J. S., Clark V. L. 1984; Conjugation of plasmids of Neisseria gonorrhoeae to other Neisseria species: potential reservoirs for the β-lactamase plasmid. Journal of Infectious Diseases 150:397–401
    [Google Scholar]
  9. Grinsted J., Bennett P. M., Higginson S., Richmond M. H. 1978; Regional preference of insertion of Tn501 and Tn802 into RP1 and its derivatives. Molecular and General Genetics 166:313–320
    [Google Scholar]
  10. Guerry P., van Embden J. D. A., Falkow S. 1974; Molecular nature of two non-conjugative plasmids carrying drug resistance genes. Journal of Bacteriology 117:619–630
    [Google Scholar]
  11. Heffron F., Rubens C., Falkow S. 1977; Transportation of a plasmid DNA sequence that mediates ampicillin resistance: identity of laboratory-constructed plasmids and clinical isolates. Journal of Bacteriology 129:530–533
    [Google Scholar]
  12. Heffron F., Kostriken R., Morita =G., Parker R. 1981; Tn3 encodes a site-specific recombination system: identification of essential sequences, genes, and the actual site of recombination. Cold Spring Harbor Symposia on Quantitative Biology 45:258–268
    [Google Scholar]
  13. Holmes D.S., Quigley M. 1981; A rapid boiling method for the preparation of bacterial plasmids. Analytical Biochemistry 114:193–197
    [Google Scholar]
  14. Meyers J. A., Sanchez O., Elwell L. P., Falkow S. 1976; Simple agarose gel electrophoresis method for the identification and characterization of plasmid DNA. Journal of Bacteriology 127:1529–1537
    [Google Scholar]
  15. Pintado C., Salvador C., Rotger R., Nombela C. 1985; Multiresistance plasmids from commensal Neisseria strains. Antimicrobial Agents and Chemotherapy 27:120–124
    [Google Scholar]
  16. Rotger R., Nombela C. 1983; Characterization of penicillin-resistant β-lactamase-producing strains of Neisseria gonorrhoeae isolated in Spain (1977–81). Microbiología Española 36:115–122
    [Google Scholar]
  17. Rotger R., Pintado C., Nombela C. 1981; Multiple transmissible antibiotic resistance in commensal Neisseria. FEMS Microbiology Letters 10:161–164
    [Google Scholar]
  18. Sakaguchi K. 1982; Vectors for gene cloning in Pseudomonas and their application. In Current Topics in Microbiology and Immunology, vol. 96, Gene Cloning in Organisms other than E. coli, pp31–45
    [Google Scholar]
  19. Southern E. 1979; Gel electrophoresis of restriction fragments. Methods in Enzymology 68:152–176
    [Google Scholar]
  20. Yamada Y., Calame K. L., Grindley J. N., Nakada D. 1979; Location of an ampicillin resistance transposon, Tn1701, in a group of small, nontransferring plasmids. Journal of Bacteriology 137:990–999
    [Google Scholar]
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