1887

Abstract

Summary: DNA-dependent RNA polymerase has been isolated from . Like those from other eubacteria, the enzyme contained four subunits: beta and beta prime ( 155000), alpha ( 38000), and sigma ( 98000). Analysis by isoelectric focussing showed that both alpha and sigma had several forms with different isoelectric pH values. The enzyme was sensitive to rifampicin (5 ng rifampicin mlgave 50% inhibition) and capable of specific promoter selection with DNA from , calf thymus and phage T7D111.

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/content/journal/micro/10.1099/00221287-132-7-1939
1986-07-01
2021-07-31
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