1887

Abstract

Summary: DNA-dependent RNA polymerase has been isolated from . Like those from other eubacteria, the enzyme contained four subunits: beta and beta prime ( 155000), alpha ( 38000), and sigma ( 98000). Analysis by isoelectric focussing showed that both alpha and sigma had several forms with different isoelectric pH values. The enzyme was sensitive to rifampicin (5 ng rifampicin mlgave 50% inhibition) and capable of specific promoter selection with DNA from , calf thymus and phage T7D111.

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/content/journal/micro/10.1099/00221287-132-7-1939
1986-07-01
2024-04-27
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References

  1. Alberts B. M., Herrick G. 1971; DNA-cellulose chromatography. Methods in Enzymology 21:198–217
    [Google Scholar]
  2. Amemiya K., Wu C. W., Shapiro L. 1977; Cauiobacter crescentus RNA polymerase purification and characterization of holoenzyme and core polymerase. Journal of Biological Chemistry 252:4157–4165
    [Google Scholar]
  3. Burgess R. R. 1969; A new method for the large scale purification of Escherichia coli deoxyribonucleic acid-dependent ribonucleic acid polymerase. Journal of Biological Chemistry 244:6160–6167
    [Google Scholar]
  4. Burgess R. R. 1976; Purification and physical properties of E. coli RNA polymerase. In RNA Polymerase pp 69–100 Edited by Losick R., Chamberlin M. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory;
    [Google Scholar]
  5. Burgess R. R., Jendrisak J. J. 1975; A procedure for the rapid, large-scale purification of Escherichia coli DNA-dependent RNA polymerase involving polymin P precipitation and DNA-cellulose chromatography. Biochemistry 14:4634–4638
    [Google Scholar]
  6. Doi R. H. 1982; RNA polymerase of Bacillus subtilis. In The Molecular Biology of the Bacilli. vol 1 Bacillus subtilis pp 72–106 Edited by Dubnau D. D. New York: Academic Press;
    [Google Scholar]
  7. Dow C. S., Whittenbury R., Carr N. G. 1983; The ‘shut-down’ or ‘growth precursor’ cell - an adaptation for survival in a potentially hostile environment. Symposia of the Society for General Microbiology 34:187–247
    [Google Scholar]
  8. Dow C. S., Kelly D. J., Oakley C. J., Porter D., Scott N. W., Swoboda U., Whittenbury R. 1985 Differentiation events in Rhodomicrobium vannielii. Annales de l’Institute Pasteur/Microbiologie 136A:19–28
    [Google Scholar]
  9. Enami M., Ishihama A. 1984; Protein phosphorylation in Escherichia coli and purification of a protein kinase. Journal of Biological Chemistry 259:526–533
    [Google Scholar]
  10. Friesen J. D., Parker J., Watson R. J., Bendiak D., Reeh S. U., Pedersen S., Fiil N. P. 1976; A transducing bacteriophage carrying the structural gene for elongation factor Ts. Molecular and General Genetics 148:93–98
    [Google Scholar]
  11. Fukuda R., Ishihama A., Saitoh T., Taketo M. 1977; Comparative studies of RNA polymerase subunits from various bacteria. Molecular and General Genetics 154:135–144
    [Google Scholar]
  12. Gonzalez N., Wiggs J., Chamberlin M. J. 1977; A single procedure for resolution of Escherichia coli RNA polymerase holoenzyme from core polymerase. Archives of Biochemistry and Biophysics 182:404–408
    [Google Scholar]
  13. Gross C., Engbaek F., Flammang T., Burgess R. 1976; Rapid micro-method for the purification of Escherichia coli ribonucleic acid polymerase and the preparation of bacterial extracts active in ribonucleic acid synthesis. Journal of Bacteriology 128:382–389
    [Google Scholar]
  14. Hayward R. S., Fyfe S. 1978; Over-synthesis and instability of sigma proteins in a merodiploid strain of Escherichia coli. Molecular and General Genetics 159:89–99
    [Google Scholar]
  15. Hinkle D. C., Chamberlin M. J. 1972; Studies of the binding of Escherichia coli RNA polymerase to DNA. (1) The role of sigma subunit in site selection. Journal of Molecular Biology 70:157–185
    [Google Scholar]
  16. Jaehning J. A., Wiggs J. L., Chamberlin M. J. 1979; Altered promoter selection by a novel form of Bacillus subtilis RNA polymerase. Proceedings of the National Academy of Sciences of the United States of America 76:5470–5474
    [Google Scholar]
  17. Kudo T., Doi R. H. 1981; Free factor of Escherichia coli RNA polymerase can bind to DNA. Journal of Biological Chemistry 256:9778–9781
    [Google Scholar]
  18. Kudo T., Jaffe D., Doi R. H. 1981; Free sigma subunit of Bacillus subtilis RNA polymerase binds to DNA. Molecular and General Genetics 181:63–68
    [Google Scholar]
  19. Laemmli U. K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature London: 227680–685
    [Google Scholar]
  20. Losick R., Pero J. 1981; Cascades of sigma factors. Cell 25:582–584
    [Google Scholar]
  21. Lotz W., Fees H., Wohlleben W., Burkardt H. J. 1981; Isolation and characterization of the DNA-dependent RNA polymerase of Rhizobium leguminosarum 300. Journal of General Microbiology 125:301–309
    [Google Scholar]
  22. Mcdonell M. W., Simon M. N., Studier F. W. 1977; Analysis of restriction fragments of T7 DNA and determination of molecular weights by electrophoresis and alkaline gels. Journal of Molecular Biology 110:119–146
    [Google Scholar]
  23. Marmur J. 1961; A procedure for the isolation of deoxyribonucleic acid from microorganisms. Journal of Molecular Biology 3:208–218
    [Google Scholar]
  24. O’Farrell P. H. 1975; High resolution two-dimensional electrophoresis of proteins. Journal of Biological Chemistry 250:4007–4021
    [Google Scholar]
  25. Ouchterlony O. 1958; Diffusion-in-gel methods for immunological analysis. In Progress in Allergy vol 5 pp 1–78 Edited by Kallos P. Basel & New York: S. Karger;
    [Google Scholar]
  26. Ovchinnikov Y. A., Lipkin V. M., Modyanov N. N., Chertov O. Y., Smirnov Y. V. 1977; Primary structure of the α-subunit of DNA-dependent RNA polymerase from E. coli. FEBS Letters 76:108–111
    [Google Scholar]
  27. Ovchinnikov Y. A., Monasturskaya G. S., Gubanov V. V., Guryev S. O., Chertov O. Y., Modyanov N. N., Grinkevich V. A., Makarova I. A., Marchenko T. V., Polovnikova I. N., Lipkin V. M., Sverdlov E. D. 1981; The primary structure of E. coli RNA polymerase. Nucleotide sequence of the rpo B gene and amino acid sequence of the β-subunit. European Journal of Biochemistry 116:621–629
    [Google Scholar]
  28. Potts L. E., Dow C. S. 1979; Nucleic acid synthesis during the developmental cycle of the Rhodomicrobium vannielii swarm cell. FEMS Microbiology Letters 6:393–395
    [Google Scholar]
  29. Regensburger B., Hennecke H. 1983; RNA polymerase from Rhizobium japonicum. Archives of Microbiology 135:103–109
    [Google Scholar]
  30. Sternbach H., Engelhardt R., Lezius A. G. 1975; Rapid isolation of highly active RNA polymerase from Escherichia coli and its subunits by matrix-bound Heparin. European Journal of Biochemistry 60:51–55
    [Google Scholar]
  31. Taylor S. C., Dow C. S. 1980; Ribulose-1,5-bisphosphate carboxylase from Rhodomicrobium vannielii. Journal of General Microbiology 116:81–87
    [Google Scholar]
  32. Wehrli W., Staehelin M. 1971; Actions of the rifamycins. Bacteriological Reviews 35:290–309
    [Google Scholar]
  33. Whittenbury R., Dow C. S. 1977; Morphogenesis and differentiation in Rhodomicrobium vannielii and other budding and prosthecate bacteria. Bacteriological Reviews 41:754–808
    [Google Scholar]
  34. Wiggs J. L., Bush J. W., Chamberlin M. J. 1979; Utilization of promoter and terminator sites on bacteriophage T7 DNA by RNA polymerases from a variety of bacterial orders. Cell 16:97–109
    [Google Scholar]
  35. Wray W., Boulikas T., Wray V. P., Hancock R. 1981; Silver staining of proteins in polyacrylamide gels. Analytical Biochemistry 118:197–203
    [Google Scholar]
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