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Volume 132, Issue 6

The Expression of the Gene in Free

Abstract

The gene was stably introduced into ϕC31-based phage cloning vectors in However, could not be stably introduced into on the plasmid vectors pIJ702 or pIJ41. Studies of the expression of in and were facilitated by the use of mutants and/or growth conditions in which endogenous β-galactosidase activity was low or absent. Plaques and lysogens of °C31:: constructs involving transcriptional fusions to the pBR322 gene contained β-galactosidase activity. Activities were markedly higher in than in Insertion of the major transcriptional terminator of coliphage fd between the promoter and reduced expression more in lysogens than in lytically infected cultures, suggesting the existence of a phage-specified anti-termination function. Several ϕC31 derivatives suitable for the construction of different kinds of transcriptional and translational fusions in were derived. The expression of in one transcriptional fusion vector (KC659) was increased both in plaques and in lysogens by the appropriately positioned insertion of a previously characterized promoter from the gene of When ϕC31 DNA fragments were inserted into KC659, at least one recombinant phage gave high β-galactosidase activity in lytic infections, but not in lysogens. The potential usefulness of fusions in is discussed.

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© Society for General Microbiology 1986
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1986-06-01
2024-04-26
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References

  1. Beck E., Sommer R., Auerswald E. A., Kurz C. L., Zink B., Osterburg G., Schaller H., Sugimoto K., Sugisaki H., Okamoto T., Takanami M. 1978; Nucleotide sequence of bacteriophage fd DNA.. Nucleic Acids Research 5:4495–4503
     [Google Scholar]
  2. Beringer J. E. 1974; R-factor transfer in Rhizobium.. Journal of General Microbiology 84:188–198
     [Google Scholar]
  3. Bibb M. J., Cohen S. N. 1982; Gene expression in Streptomyces: construction and application of pro- moter-probe plasmid vectors in Streptomyces lividans.. Molecular and General Genetics 187:265–277
     [Google Scholar]
  4. Bibb M. J., Freeman R. F., Hopwood D. A. 1977; Physical and genetical characterisation of a second sex factor, SCP2, for Streptomyces coelicolor A3(2).. Molecular and General Genetics 154:155–166
     [Google Scholar]
  5. Bibb M. J., Bibb M. J., Ward J. M., Cohen S. N. 1985; Nucleotide sequences encoding and promoting expression of three antibiotic resistance genes indigenous to Streptomyces.. Molecular and General Genetics 199:26–36
     [Google Scholar]
  6. Bolivar F., Rodriguez R. L., Green P. J., Betlach M. C., Heyneker H. L., Boyer H. W., Crosa J. H., Falkow S. 1977; Construction and characterisation of new cloning vehicles. II. A multipurpose cloning system.. Gene 2:95–113
     [Google Scholar]
  7. Casadaban M. J., Chou J., Cohen S. N. 1980; In vitro gene fusions that join an enzymatically active /i-galactosidase segment to amino-terminal fragments of exogenous proteins: Escherichia coli plasmid vectors for the detection and cloning of translational initiation signals.. Journal of Bacteriology 143:971–980
     [Google Scholar]
  8. Chater K. F. 1984; Morphological and physiological differentiation in Streptomyces.. In Microbial Development pp 89–115 Editor Losick R, Shapiro L. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory.;
     [Google Scholar]
  9. CHATER K. F., WlLDE L. C. 1980; Streptomyces albus G mutants defective in the SalGI restriction- modification system.. Journal of General Microbiologv 116:323–334
     [Google Scholar]
  10. Chater K. F., Bruton C. J., Springer W., Suarez J. E. 1981; Dispensable sequences and packaging constraints of DNA from the Streptomyces temperate phage µC3!.. Gene 15:249–256
     [Google Scholar]
  11. Chater K. F., Bruton C. J., King A. A., Suarez J. E. 1982; The expression of Streptomyces and Escherichia coli drug resistance determinants cloned into the Streptomyces phage 0C 31.. Gene 19:21–32
     [Google Scholar]
  12. Chater K. F., King A. A., Rodicio M. R., Bruton C. J., Fisher S. H., Piret J. M., Smith C. P., Foster S. G. 1985; Cloning and analysis of Streptomyces DNA in C31 -derived vectors.. In Microbiology 1 985: pp 421–426 Editor Hegemon G., Hershberger C. L. Washington, DC: American Society for Microbiology;
     [Google Scholar]
  13. Chinenova T. A., Mkrtumian N. M., Lomov- skaya N. D. 1982; Genetic study of a novel phage resistance character in Streptomyces coelicotor A3(2).. Genetika 18:1945–1952
     [Google Scholar]
  14. Eckhardt T. G., Fare L. R. 1982; /i-Galactosi- dase genes in Streptomyces lividans.. In Abstract Book, Fourth International Symposium on Genetics of Industrial Microorganisms (Kyoto, Japan, June 1982) p. 36
     [Google Scholar]
  15. Fayerman J. T., Jones M. D., Richardson M. A. 1985; Development of systems for heterologous gene expression in Streptomyces spp.. In Microbiology 1 985: pp 414–420 Editor Hegemon G, Hershberger. C. L. Washington, DC: American Society for Microbiology;
     [Google Scholar]
  16. Friedman D. I., Gottesman M. 1983; Lytic mode of lambda development.. In Lambda II pp 21–51 Editor Hendrix R. W., Stahl J. W., Weisberg. R. A. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory;
     [Google Scholar]
  17. Gentz R., Langner A., Chang A. C. Y., Cohen S. N., Bujard H. 1981; Cloning and analysis of strong promoters is made possible by the downstream placement of an RNA termination signal.. Proceedings of the National Academy of Sciences of the United States of America 78:4931–4940
     [Google Scholar]
  18. Guarente L., Lauer G., Roberts T. M., Ptashne M. 1980; Improved methods for maximizing expresson of a cloned gene: a bacterium that synthesizes rabbit /J-globin.. Cell 20:543–553
     [Google Scholar]
  19. Harris J. E., Chater K. F., Bruton C. J., Piret J. M. 1983; The restriction mapping and use in cloning vector development of c gene deletions of Streptomyces bacteriophage C31. Gene 22:167–174
     [Google Scholar]
  20. Herbert D., Phipps P. J., Strange R. E. 1971; Chemical analysis of microbial cells.. Methods in Microbiology 5B209–344
     [Google Scholar]
  21. Hopwood D. A., Wright H. M. 1973; Transfer of a plasmid between Streptomycs species.. Journal of General Microbiology 77:187–195
     [Google Scholar]
  22. Hopwood D. A., Lydiate D. J., Malpartida F., Wright H. M. 1985a; Conjugative sex plasmids of Streptomyces.. In Plasmids in Bacteria pp. 615–643 Editor Helinski S. N., Cohen D. B., Clewell D. A., Jackson D. A., Hollaender. A. New York: Plenum Press;
     [Google Scholar]
  23. Hopwood D. A., Bruton C. J., Chater K. F., Feitelson J. S., Gil J. A., Jones G. H., Lydiate D. J., Malpartida F. 1985b; Cloning of antibiotic biosynthesis genes in Streptomyces.. In Genetics: New Frontiers. Proceedings of the XV International Congress of Genetics Recombinant DNA Technology vol,2: pp. 3–16 Editor Chopra B. C., Joshi R. P., Sharma V.L., Bansal H. C. New Delhi: Oxford & IBH Publishing Co;
     [Google Scholar]
  24. Hopwood D. A., Bibb M. J., Chater K. F., Kieser T., Bruton C. J., Kieser H. M., Lydiate D. J., Smith C. P., Ward J. M., Schrempf H. 1985c; Genetic Manipulation of Streptomyces:. A Laboratory Manual. Norwich, UK: The John Innes Foundation;
     [Google Scholar]
  25. Janssen G. R., Bibb M. J., Smith C. P., Ward J. M., Kieser T., Bibb M. J. 1985; Isolation and analysis of Streptomyces promoters.. In Microbiology 1 985: pp 392–396 Editor Hegemon G., Hershberger C. L. Washington, DC: American Society for Microbiology;
     [Google Scholar]
  26. Jaurin B., Cohen S. N. 1984; Streptomyces lividans RNA polymerase recognizes and uses Escherichia coli transcription signals.. Gene 28:83–91
     [Google Scholar]
  27. Katz E., Thompson C. J., Hopwood D. A. 1983; Cloning and expression of the tyrosinase gene from Streptomyces antibioticus in Streptomyces lividans.. Journal of General Microbiology 129:2703–2714
     [Google Scholar]
  28. KING A. A. 1983; Streptomyces gene fusions involving the Escherichia coli (i-galactosidase gene.. PhD thesis University of East Anglia: Norwich, UK;
     [Google Scholar]
  29. Lomovskaya N. D., Mkrtumian N. M., , Gostim- skaya N. L., Danilenko V. N. 1972; Characterisation of temperate actinophage 0C31 isolated from Streptomyces coelicolor A3(2).. Journal of Virology 9:258–262
     [Google Scholar]
  30. MANIATIS T., FRISCH E. F., SAMBROOK J. 1982; Molecular Cloning: a Laboratory Manual.. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory.;
     [Google Scholar]
  31. MILLER J. 1972; Experiments in Molecular Genetics.. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory.;
     [Google Scholar]
  32. Mitchell D. H., Reznikoff W. S., Beckwith J. R. 1975; Genetic fusions defining trp and lac operon regulatory elements.. Journal of Molecular Biology 93:331–350
     [Google Scholar]
  33. Murray N. E., Brammar W. J., Murray K. 1977; Lambdoid phages that simplify the recovery of in vitro recombinants.. Molecular and General Genetics 150:53–61
     [Google Scholar]
  34. Piret J. M., Chater K. F. 1985; Phage-mediated cloning of bldA, a region involved in Streptomyces coelicolor morphological development, and its analysis by genetic complementation.. Journal of Bacteriology 163:965–972
     [Google Scholar]
  35. Rodicio M. R., Bruton C. J., Chater K. F. 1985; New derivatives of the Streptomyces temperate phage $C31 useful for cloning and functional analysis of Streptomyces DNA.. Gene 34:283–292
     [Google Scholar]
  36. Seno E. T., Chater K. F. 1983; Glycerol catabolic enzymes and their regulation in wild-type and mutant strains of Streptomyces coelicolor A3(2).. Journal of General Microbiology 129:1403–1413
     [Google Scholar]
  37. Shapira S. K., Chou J., Richaud F. V., Casadaban M. J. 1983; New versatile plasmid vectors for expression of hybrid proteins coded by a cloned gene fused to lacZ gene sequences encoding an enzymatically active carboxy-terminal portion of /J-galactosidase.. Gene 25:71–82
     [Google Scholar]
  38. SILHAVY T. J., BERNAN M. L., ENQUIST L. W. 1984; Experiments with Gene Fusions.. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory.;
     [Google Scholar]
  39. Suarez J. E., Chater K. F. 1980; DNA cloning in Streptomyces: a bifunctional replicon comprising pBR322 inserted into a Streptomyces phage.. Nature, London 286:527–529
     [Google Scholar]
  40. Thompson C. J., Gray G. S. 1983; Nucleotide sequence of a streptomycete aminoglycoside phosphotransferase gene and its relationship to phosphotransferases encoded by resistance plasmids.. Proceedings of the National Academy of Sciences of the United States of America 80:5190–5194
     [Google Scholar]
  41. Thompson C. J., Kieser T., Ward J. M., Hopwood D. A. 1982a; Physical analysis of antibiotic-resistance genes from Streptomyces and their uses in vector construction.. Gene 20:51–62
     [Google Scholar]
  42. THOMPSON C. J., WARD J. M., HOPWOOD D. A. 1982b; Cloning of antibiotic resistance and nutritional genes in streptomycetes.. Journal of Bacteriology 151:668–677
     [Google Scholar]
  43. WARD J. M., JANSSEN G. R., KIESER T., BIBB M. J., BUTTNER M. J., BIBB M. J. 1986; Construction and characterisation of a series of multi-copy promoter-probe plasmid vectors for Streptomyces using the aminoglycoside phosphotransferase gene from Tn5 as indicator.. Molecular and General Genetics (in the Press).
     [Google Scholar]
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The Expression of the Escherichia coli lacZ Gene in Streptomyces
Microbiology 132, 1739 (1986); https://doi.org/10.1099/00221287-132-6-1739
/content/journal/micro/10.1099/00221287-132-6-1739
/content/journal/micro/10.1099/00221287-132-6-1739
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