1887

Abstract

The gene was stably introduced into ϕC31-based phage cloning vectors in However, could not be stably introduced into on the plasmid vectors pIJ702 or pIJ41. Studies of the expression of in and were facilitated by the use of mutants and/or growth conditions in which endogenous β-galactosidase activity was low or absent. Plaques and lysogens of °C31:: constructs involving transcriptional fusions to the pBR322 gene contained β-galactosidase activity. Activities were markedly higher in than in Insertion of the major transcriptional terminator of coliphage fd between the promoter and reduced expression more in lysogens than in lytically infected cultures, suggesting the existence of a phage-specified anti-termination function. Several ϕC31 derivatives suitable for the construction of different kinds of transcriptional and translational fusions in were derived. The expression of in one transcriptional fusion vector (KC659) was increased both in plaques and in lysogens by the appropriately positioned insertion of a previously characterized promoter from the gene of When ϕC31 DNA fragments were inserted into KC659, at least one recombinant phage gave high β-galactosidase activity in lytic infections, but not in lysogens. The potential usefulness of fusions in is discussed.

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1986-06-01
2021-07-30
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