1887

Abstract

Analysis of human gut contents showed that substantial quantities of soluble protein, ammonia and branched chain volatile fatty acids occurred throughout the large intestine [0·1-24·4 g (kg contents), 7·7-66·0 mmol (kg contents) and 1·5-11·1 mmol (kg contents) respectively]. The presence of these metabolites suggested that substantial proteolysis was occurring. studies showed that casein and bovine serum albumin were partly degraded in slurries of human faeces over a 96 h incubation period, to produce TCA-soluble peptides, ammonia and volatile fatty acids. Proteolytic activity detected in the stools of five individuals ranged from 3·5 to 19·8 mg azocasein hydrolysed h (g faecal material). Washed cell and washed particulate faecal fractions accounted for 24-67% of total activity. The predominant proteolytic bacteria in the faecal samples examined were identified as spp. [1·0 × 10-1·3 × 10 (g dry wt faeces)] and spp. [1·2 × 10-1·0 × 10 (g dry wt faeces)]. Other proteolytic bacteria which occurred in lesser numbers were identified as belonging to the genera and These results demonstrate that the gut microflora could potentially play a major role in proteolysis in the human colon.

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1986-06-01
2024-12-02
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References

  1. Allison M. J. 1978; Production of branched-chain volatile fatty acids by certain anaerobic bacteria.. Applied and Environmental Microbiology 35:872–877
    [Google Scholar]
  2. Appleby J. C. 1955; The isolation and classification of proteolytic bacteria from the rumen of the sheep.. Journal of General Microbiology 12:526–533
    [Google Scholar]
  3. Blackburn T. H. 1968; Protease production by Bacteroides amylophilus strain H 18. Journal of General Microbiology 53:27–36
    [Google Scholar]
  4. BLACKBURN T. H., HOBSON P. N. 1960; Proteolysis in the sheep rumen by whole and fractionated rumen contents.. Journal of General Microbiology 22:272–281
    [Google Scholar]
  5. Blackburn T. H., Hobson P. N. 1962; Further studies on the isolation of proteolytic bacteria from the sheep rumen.. Journal of General Microbiology 29:69–81
    [Google Scholar]
  6. Brock F. M., , Forsberg C. W., Buchanan-Smith J. G. 1982; Proteolytic activity of rumen microorganisms and effects of proteinase inhibitors.. Applied and Environmental Microbiology 44:561–569
    [Google Scholar]
  7. Cowan S. T. 1974; Manual for the Identification of Medical Bacteria.. Cambridge: Cambridge University Press.;
    [Google Scholar]
  8. CUMMINGS J. H. 1981; Short chain fatty acids in the human colon. . Gut 22:763–779
    [Google Scholar]
  9. Cummings J. H., , Hill M. J., , Bone E. S., , Branch W. J., Jenkins D. J. A. 1979; The effect of meat protein and dietary fiber on colonic function and metabolism. Part II. Bacterial metabolites in faeces and urine.. American Journal of Clinical Nutrition 32:2094–2101
    [Google Scholar]
  10. Drasar B. S., Hill M. J. 1974; Human Intestinal Flora.. London & New York: Academic Press.;
    [Google Scholar]
  11. Fulghum R. S., Moore W. E. C. 1963; Isolation, enumeration, and characteristics of proteolytic ruminal bacteria. . Journal of Bacteriology 85:808–815
    [Google Scholar]
  12. Hazlewood G. P., Edwards R. 1981; Proteolytic activities of a rumen bacterium, Bacteroides rumini- cola R8/4. Journal of General Microbiology 125:11–15
    [Google Scholar]
  13. Hespell R. B., Smith C. J. 1983; Utilization of nitrogen sources by gastrointestinal tract bacteria. . in Human Intestinal Microflora in Health and Disease, PP 167–187 Editor Hentges D. J. London& New York: Academic Press.;
    [Google Scholar]
  14. Holdeman L. V., , Cato E. P., Moore W. E. C. (editors) 1977; Anaerobic Laboratory Manual, 4th edn. Blacksburg, Virginia: VPI Anaerobe Laboratory..
    [Google Scholar]
  15. Ingram E., , Holland K. T., , Gowland G., Cunliffe W. J. 1983; Studies of the extracellular proteolytic activity produced by Propionibacterium acnes.. Journal of Applied Bacteriology 54:263–271
    [Google Scholar]
  16. Macfarlane G. T., Allison C. 1985; Utilization of protein by human gut bacteria.. Journal of Applied Bacteriology 59:xix.
    [Google Scholar]
  17. Macfarlane G. T., , Allison C., Cummings J. H. 1985; Distribution of proteolytic activity in the intestinal contents of man.. Journal of Applied Bacteriology 59:xviii–xix.
    [Google Scholar]
  18. Malik A. C., , Reinhold G. W., Vedamuthu E. R. 1968; An evaluation of the taxonomy of Propioni- bacterium.. Canadian Journal of Microbiology 14:1185–1191
    [Google Scholar]
  19. Mangan J. L. 1972; Quantitative studies on nitrogen metabolism in the bovine rumen. The rate of proteolysis of casein and ovalbumin and the release and metabolism of free amino acids.. British Journal of Nutrition 27:261–283
    [Google Scholar]
  20. Martley F., G„ Jayashankar S. R., Lawrence R. C. 1970; An improved agar medium for the detection of proteolytic organisms in total bacterial counts.. Journal of Applied Bacteriology 33:363–370
    [Google Scholar]
  21. Moore W. E. C., Holdeman L. V. 1974; Human fecal flora: the normal flora of 20 Japanese- Hawaiians.. Applied and Environmental Microbiology 27:961–979
    [Google Scholar]
  22. Nugent J. H. A., Mangan J. L. 1978; Rumen proteolysis of fraction 1 leaf protein, casein and bovine serum albumin.. Proceedings of the Nutrition Society 37:48A.
    [Google Scholar]
  23. Prins R. A. 1977; Biochemical activities of gut microorganisms. . in Microbial Ecology of the Gut PP 73–183 Editor Clarke R. T. J., Bauchop. T. London& New York: Academic Press.;
    [Google Scholar]
  24. Sabbaj J., , Sutter V. L., Finegold S. M. 1970; Urease and deaminase activities in fecal bacteria in hepatic coma. . in Antimicrobial Agents and Chemotherapy PP 181–185 Editor Hobby G. L. Washington, DC: American Society for Microbiology.;
    [Google Scholar]
  25. Salyers A. A., Leedle J. A. Z. 1983; Carbohydrate metabolism in the human colon.. in Human Intestinal Microflora in Health and Disease, PP 129–146 Editor Hentges. D. J. London: New York: Academic Press.;
    [Google Scholar]
  26. Salyers A. A., , Gherardini F., O’Brien M. 1981; Utilization of xylan by two species of human colonic Bacteroides.. Applied and Environmental Microbiology 41:1065–1068
    [Google Scholar]
  27. Solorzano L. 1969; Determination of ammonia in natural waters by the phenolhypochlorite method. Limnology and Oceanography 14:799–801
    [Google Scholar]
  28. SUMMERSKILL W. H. J., WOLPERT E. 1970; Ammonia metabolism in the gut.. American Journal of Clinical Nutrition 22:633–639
    [Google Scholar]
  29. Varel V. H., , Bryant M. P., , Holdeman L. V., Moore W. E. C. 1974; Isolation of ureolytic Peptostreptococcus productus from feces using defined medium: failure of common urease tests.. Applied Microbiology 28:394–399
    [Google Scholar]
  30. Vince A., , Down P. F., , Morison J., , Twigg F. J., Wrong O. M. 1976; Generation of ammonia from non-urea sources in a faecal incubation system.. Journal of Clinical Science and Molecular Medicine, 51:313–322
    [Google Scholar]
  31. Wallace R. J., Brammall M. L. 1985; The role of different species of bacteria in the hydrolysis of protein in the rumen.. Journal of General Microbiology 131:821–832
    [Google Scholar]
  32. Wiggins H. S. 1983; Gastroenterological functions of dietary fibre. . in Dietary Fibre PP 205–219 Editor Birch G. G., Parker. K. J. London: Applied Science Publishers.;
    [Google Scholar]
  33. Wilson D. R., , Ing T. S., , Metcalfe- Gibson A., Wrong O. M. 1968; In vivo dialysis of faeces as a method of stool analysis. III. The effect of intestinal antibiotics.. Clinical Science 34:211–221
    [Google Scholar]
  34. Wrong O. M., , Vince A. J., Waterlow J. C. 1985; The contribution of endogenous urea to faecal ammonia in man, determined by 15N labelling of plasma urea.. Clinical Science 68:193–199
    [Google Scholar]
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