1887

Abstract

Pigmented and non-pigmented strains of produced extracellular vesicles and had wetting activity when grown at 30°C but not at 37°C. Light microscopy showed that the red pigment was present in vesicles and intracellular granules. Electron microscopy revealed the presence of vesicles surrounded by the bacterial membrane. Three lipids having the wetting activity, W1, W2 and W3, were isolated by thin-layer chromatography of lipids from different strains of . Dispersions of the isolated wetting agents had small contact angles on a polystyrene surface and the ability to lower surface tension. Wetting agent W1 was the aminolipid serratamolide. Wetting agents W2 and W3 were also aminolipids but were shown to be different from serratamolide by chemical analyses. Wetting agent and prodigiosin (in a pigmented strain) were the main lipids of isolated vesicles.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-132-4-865
1986-04-01
2021-07-29
Loading full text...

Full text loading...

/deliver/fulltext/micro/132/4/mic-132-4-865.html?itemId=/content/journal/micro/10.1099/00221287-132-4-865&mimeType=html&fmt=ahah

References

  1. Blanchard D. C., Syzdek K. D. 1978; Seven problems in bubble and jet drop researches.. Limnology and Oceanography 23:389–400
    [Google Scholar]
  2. Grimont P. A. D., Grimont F. 1978; The genus Serratia.. Annual Review of Microbiology 32:221–248
    [Google Scholar]
  3. Heller K. B. 1979; Lipolytic activity copurified with the outer membrane of Serratia marcescens.. Journal of Bacteriology 140:1120–1122
    [Google Scholar]
  4. Hermansson M., Kjelleberg S., Norkrans B. 1979; Interaction of pigmented wildtype and pigmentless mutant of Serratia marcescens with lipid surface film.. FEMS Microbiology Letters 6:129–132
    [Google Scholar]
  5. Kawai Y., YaNO I. 1983; Ornithine-containing lipid of Bordetella pertussis, a new type of hemagglutinin.. European Journal of Biochemistry 136:531–538
    [Google Scholar]
  6. KJELLEBERG S., LAGERCRANTZ C., LARSSON TH. 1980; Quantitative analysis of bacterial hydropho- bicity studied by the binding of dodecanoic acid. FEMS Microbiology Letters 7:41–44
    [Google Scholar]
  7. Kobayashi T., Grossman G., Robertson B., Ueda T. 1984; Effects of artificial and natural surfactant supplementation in immature newborn rabbits.. Journal of the Japanese Medical Association of Biological Interface 15:53–59
    [Google Scholar]
  8. Kole M. M., Altosaar I. 1985; Increased chitinase production by a non-pigmented mutant of Serratia marcescens.. FEMS Microbiology Letters 26:265–269
    [Google Scholar]
  9. Mack G. L. 1935; The determination of contact angles from measurements of the dimensions of small bubbles and drops. I. The spheroidal segment method for acute angles.. Journal of Physical Chemistry 40:159–167
    [Google Scholar]
  10. Marrie T. J., Costerton J. W. 1981; Prolonged survival of Serratia marcescens in chlorhexidine.. Applied and Environmental Microbiology 42:1093–1102
    [Google Scholar]
  11. Matsuyama T. 1977; Resistance of Bordetella pertussis phase I to mucociliary clearance by rabbit tracheal mucous membrane.. Journal of Infectious Diseases 136:609–616
    [Google Scholar]
  12. Matsuyama T., Fujita M., Yano I. 1985; Wetting agent produced by Serratia marcescens.. FEMS Microbiology Letters 28:125–129
    [Google Scholar]
  13. Murakami T., Matsuyama T. 1980; Growth of Bacteroides fragilis inoculated on rabbit tracheal explant in an atmospheric environment.. Journal of Infectious Diseases 142:332–337
    [Google Scholar]
  14. Ohno Y., Yano I., Hiramatsu T., Masui M. 1976; Lipids and fatty acids of a moderately halophilic bacterium, no.101. Biochimica et biophy- sica acta 424:337–350
    [Google Scholar]
  15. Purkayastha M., Williams R. P. 1960; Association of pigment with the cell envelope of Serratia marcescens (Chromobacterium prodigiosum).. Nature, London 187:349–350
    [Google Scholar]
  16. Rosenberg M. 1981; Bacterial adherence to polystyrene : a replica method of screening for bacterial hydrophobicity.. Applied and Environmental Microbiology 42:375–377
    [Google Scholar]
  17. Wasserman H. H., Keggi J. J., McKeon J. E. 1961; Serratamolide, a metabolic product of Serratia.. Journal of the American Chemical Society 83:4107–4108
    [Google Scholar]
  18. Wasserman H. H., Keggi J. J., McKeon J. E. 1962; The structure of serratamolide.. Journal of the American Chemical Society 84:2978–2982
    [Google Scholar]
  19. Williams R. P., Qadri S. M. H. 1980; The pigment of Serratia.. In The Genus Serratia pp 3175 Editor von Graevenitz A., Rubin S. J. Boca Raton, Florida: CRC Press.;
    [Google Scholar]
  20. Winkler U., Heller K. B., Folle B. 1978; Pleiotropic consequences of mutations towards antibiotic-hypersensitivity in Serratia marcescens.. Archives of Microbiology 116:259–268
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-132-4-865
Loading
/content/journal/micro/10.1099/00221287-132-4-865
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error