SUMMARY: The development of an optimized polyuridylic acid-dependent polyphenylalanine-synthesizing system using cell-free extracts of the basidiomycete fungus is described. The assay has been used to show that cycloheximide-resistant strains CY8.2, CY9.23 and Sp98, all mutant at the -2 locus, have cytoplasmic ribosomes which are more resistant to the drug than the corresponding sensitive strains, CY8, CY9 and CY3. Cycloheximide concentrations and molar ratios of cycloheximide to ribosomes required for 50% inhibition under standard assay conditions are presented for these strains. The molar ratio required for 50% inhibition is dependent on the concentration of ribosomes in the assay.


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