1887

Abstract

SUMMARY: Two forms of citrate synthase (EC 4.1.3.7) have been found in several species of , a ‘large’ form ( ≃ 250000) which is generally inhibited by NADH and reactivated by AMP, and a ‘small’ form ( ≃ 100000) which is insensitive to these nucleotide effectors. Other species of were found to contain either the ‘large’ or the ‘small’ form. Gel filtration and ion-exchange with the technique of fast protein liquid chromatography were used to resolve the enzymes. Where both citrate synthases were present, there did not appear to be an equilibrium between the two forms. The results reveal a new and complex diversity of citrate synthase within the genus

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-132-3-737
1986-03-01
2022-01-20
Loading full text...

Full text loading...

/deliver/fulltext/micro/132/3/mic-132-3-737.html?itemId=/content/journal/micro/10.1099/00221287-132-3-737&mimeType=html&fmt=ahah

References

  1. Danson M. J., Weitzman P. D. J. 1973; Functional groups in the activity and regulation of Escherichia coli citrate synthase. Biochemical Journal 135:513–524
    [Google Scholar]
  2. Higa A. I.., Massarini E., Cazzulo J. J. 1978; Purification and some properties of the citrate synthase from a marine Pseudomonas. Canadian Journal of Microbiology 24:215–221
    [Google Scholar]
  3. Massarini E., Cazzulo J. J. 1975; Two forms of citrate synthase in a marine pseudomonad. FEBS Letters 57:134–138
    [Google Scholar]
  4. Mitchell C. G., O’Neil S., Reeves H. C., Weitzman P. D. J. 1986; Isoenzyme separation by fast protein liquid chromatography. FEBS Letters (in the Press)
    [Google Scholar]
  5. Palleroni N. J. 1978; Patterns of ProgressThe Pseudomonas Group. Shildon, UK: Meadowfield Press.
    [Google Scholar]
  6. Skinner A. J., Clarke P. H. 1968; Acetate and acetamide mutants of Pseudomonas aeruginosa 8602. Journal of General Microbiology 50:183–194
    [Google Scholar]
  7. Solomon M., Weitzman P. D. J. 1983; Occurrence of two distinct citrate synthases in a mutant of Pseudomonas aeruginosa and their growth-dependent variation. FEBS Letters 155:157–160
    [Google Scholar]
  8. Weitzman P. D. J. 1980; Citrate synthase and succinate thiokinase in classification and identification. In Microbiological Classification and Identification, pp. 107–125 Edited by M. Goodfellow & R. G. Board. London & New York: Academic Press.
    [Google Scholar]
  9. Weitzman P. D. J. 1981; Unity and diversity in some bacterial citric acid cycle enzymes. Advances in Microbial Physiology 22:185–244
    [Google Scholar]
  10. Weitzman P. D. J., Dunmore P. 1969; Citrate synthases: allosteric regulation and molecular size. Biochimica et biophysica acta 171:198–200
    [Google Scholar]
  11. Wright J. A., Sanwal B. D. 1971; Regulatory mechanisms involving nicotinamide adenine nucleotides as allosteric effectors. IV. Physicochemical study and binding of ligands to citrate synthase. Journal of Biological Chemistry 246:1689–1699
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-132-3-737
Loading
/content/journal/micro/10.1099/00221287-132-3-737
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error