1887

Abstract

Summary: Intact cells of phosphate-depleted possessed non-specific alkaline phosphatase (EC 3.1.3.1; PMEase) and 5′-nucleotidase (EC 3.1.3.5; 5′-NDase) activities; there was also an extracellular PMEase. The optimum pH for the cell-bound PMEase was > 10.3, and for the 5′-NDase was 9–9.5. The extracellular PMEase had an optimum pH > 10.3 and accounted for > 30% of the total PMEase activity at this pH; there was no extracellular 5′-NDase activity. The activities of these enzymes increased during phosphate-deprivation, but the rate of AMP hydrolysis (by the action of both PMEase and 5′-NDase) always exceeded that of -nitrophenylphosphate at the physiological pH (for a marine organism) of 8–8.5. By the use of differential centrifugation after cell disruption in a French pressure cell, a highly purified fraction of cell walls was prepared. This fraction was virtually devoid of membranous material as viewed by electron microscopy, and exhibited PMEase, but no 5′-NDase activity. By using a different centrifugation procedure after disruption by shaking with glass beads, a microsomal fraction (pelleted by forces of 14000–156000 ) was prepared. This fraction was free of cell wall fragments as viewed by electron microscopy, and exhibited 5′-NDase activity but no PMEase activity. It is concluded that the PMEase was associated with cell walls, whilst the membrane-bound 5′-NDase which sedimented as vesicles in the microsomal fraction was associated with plasma membranes.

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1986-02-01
2021-09-24
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References

  1. Chiaudani G., Vighi M. 1982; Multistep approach to identification in Northern Adriatic eutro-phied coastal waters. Water Research 16:1161–1166
    [Google Scholar]
  2. Doonan B. B., Jensen T. E. 1982; Ultrastructural localization of alkaline phosphatase in the blue-green bacterium Plectonema boryanum. Journal of Bacteriology 132:967–973
    [Google Scholar]
  3. Dracup M. N. H., Barrett-Lennard E. G., Greenway H., Robson A. D. 1984; Effect of phosphorus deficiency on phosphatase activity of cell walls from roots of subterranean clover. . Journal of Bacteriology 35:466–480
    [Google Scholar]
  4. Flynn K. J., Syrett P. J. 1984; The development of the ability to take up L-lysine by the diatom Phaeodactvlum tricornutum. . Marine Biology 89:317–325
    [Google Scholar]
  5. Gilkes K. J., Weeks G. 1977; The purification and characterization of Dictyostelium discoideum plasma membranes. Biochimicu et biophysica acta 464:142–256
    [Google Scholar]
  6. Goldman J.C., McCarthy J.J., Peavey D.G. 1979; Growth rate influence on the chemical composition of phytoplankton in oceanic waters. Nature, London 279:210–215
    [Google Scholar]
  7. Karl D. M., Craven D. B. 1979; Effect of alkaline phosphatase activity on nucleotide measurements in aquatic microbial communities. Applied and Environmental Microbiology 40:549–561
    [Google Scholar]
  8. Kuenzler E. J. 1965; Glucose-6-phosphate utilization by marine algae. Journal of Phycology 1:156–158
    [Google Scholar]
  9. Kuenzler E. J., Perras J. P. 1965; Phosphatases of marine algae. Biological Bulletin 128:271–284
    [Google Scholar]
  10. Lanzetta P. A., Alvarez L. J., Reinach P. S., Candia O. A. 1965; An improved assay for nanomole amounts of inorganic phosphate . Analytical Biochemistry 100:95–97
    [Google Scholar]
  11. Lee A., Chance K., Weeks C., Weeks G. 1975; Studies on the alkaline phosphatase and 5'-nucleoti-dase of Dictyostelium discoideum. Archives of Biochemistry and Biophysics 171:405–417
    [Google Scholar]
  12. Leonard R. T., Hodges T. K. (1980); The plasma membrane. In The Biochemistry of Plants, 1. The Plant Cell, Edited by N. E. Tolbert. London: Academic Press. 163–182
    [Google Scholar]
  13. McGrath S. M., Sullivan C. W. (1981); Community metabolism of adenylates by microheterotrophs from the Los Angeles Harbor and Southern California coastal waters. Marine Biology 62:217–226
    [Google Scholar]
  14. Moller M., Myklestad S., Haug A. (1975); Alkaline and acid phosphatase of the ma ine diatoms Chaetoceros affinis var. willei (Gran) Hustedt and Skelelonema coslatum (Grev.) Cleve. Journal of Experimental Marine Biology and Ecology 19:217–226
    [Google Scholar]
  15. Myklestad S., Sakshaug E. 1983; Alkaline phosphatase activity of Skeletonema costatum populations in the Trondheimsfjord. Journal of Plankton Research 5:557–564
    [Google Scholar]
  16. Patri N. J., Billmire E., Aaronson S. 1974; Isolation of the Ochromonas danica plasma membrane and identification of several membrane fractions. Biochimica et biophysica acta 373:347–355
    [Google Scholar]
  17. Perry M. J. 1972; Alkaline phosphatase activity in subtropical central North Pacific waters using a sensitive fluorimetric method. Marine Biology 15:113–119
    [Google Scholar]
  18. Provasoli L, McLaughlin J. J. A., Droop M. R. 1957; The development of artificial media for marine algae. Archiv fur Mikrobiologie 25:392–428
    [Google Scholar]
  19. Rhee G.-Y. 1972; Competition between an alga and an aquatic bacterium for phosphate. Limnology and Oceanography 17:505–514
    [Google Scholar]
  20. Sakshaug F., Graneli E., Elbrachter M., Kayser H. 1984; Chemical composition and alkaline phosphatase activity of nutrient saturated and phosphorus deficient cells of four marine dinoflagellates. Journal of Experimental Marine Biology and Ecology 77:241–254
    [Google Scholar]
  21. Smith R. E. H., Kalff J. 1981; The effect of phosphorus limitation on algal growth rates: evidence from alkaline phosphatase. Canadian Journal of Fisheries and Aquatic Sciences 38:1421–1427
    [Google Scholar]
  22. Solorzano L., Strickland J. D. H. 1968; Polyphosphate in sea water. Limnology and Oceanography 13:515–518
    [Google Scholar]
  23. Stewart A. J., Wetzel R. G. 1982; Influence ot dissolved humic materials on carbon assimilation and alkaline phosphatase activity in natural algal bacterial assemblages. Freshwater Biology 12:369–380
    [Google Scholar]
  24. Sullivan C. W., Volcani B. E. 1974; Isolation and characterization of plasma and smooth membranes of the marine diatom Nitzschia alba. Archives of Biochemistry and Biophysics 163:29–45
    [Google Scholar]
  25. Taft J. L., Loftus M. E., Taylor W. R. 1977; Phosphate uptake from phosphomonoesters by phytoplankton in the Chesapeake Bay. Limnology and Oceanography 22:1012–1021
    [Google Scholar]
  26. Yoshida S., Uemura M., Niki T., Sakai A., Gusta L. V. 1983; Partition of membrane particles in an aqueous two-polymer phase system and its practical use for purification of plasma membranes from plants. Plant Physiology 72:105–114
    [Google Scholar]
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