1887

Abstract

Summary: A mutant of defective in β-isopropylmalate dehydrogenase was isolated. It was transformed with YEp13, a shuttle vector containing the 2 gene, either using the protoplasting method or by treatment with LiCl. Leu transformants were recovered at a frequency of 30-60 μg DNA). The transformants were shown to contain an autonomously replicating plasmid by stability tests, hybridization experiments and by the recovery of YEp13 from transformed cells via transformation of . YEp13 linearized by HI digestion was also effective in transformation; the linearized plasmid was shown to recircularize and replicate autonomously in the transformants.

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1986-12-01
2022-01-22
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