1887

Abstract

SUMMARY: The modification status of DNA throughout the life cycle of strain 26 was analysed by restriction analysis with methylation-sensitive enzymes. A significant fraction of the GATC sequences ( target) in spore DNA contain -methyladenine, a modification that is lacking during the vegetative phase. From the modulation of the modification pattern of GATC sites, the existence of a methylase may be inferred. Spore DNA was more sensitive than vegetative cell DNA to HI, I, I and I, indicating that the sites for these enzymes are modified during the vegetative growth phase.

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1986-10-01
2021-07-25
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