1887

Abstract

SUMMARY: Mutants of impaired in lithoautotrophic metabolism (Aut) were obtained by Tn5 mutagenesis, mitomycin C treatment or incubation at sublethal temperature. A colony assay provided a fast screening method for hydrogenase-negative mutants. Three classes of mutants were identified: the first had lost the ability to grow autotrophically with H and heterotrophically with NO as nitrogen source; the second lacked both hydrogenase and ribulose-1,5-bisphosphate carboxylase activity but nitrate metabolism was unaffected; the third retained the activities of the key enzymes of lithoautotrophic metabolism but failed to grow with H and CO. All of the mutants showed the plasmid pattern of the wild-type.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-132-1-91
1986-01-01
2021-05-16
Loading full text...

Full text loading...

/deliver/fulltext/micro/132/1/mic-132-1-91.html?itemId=/content/journal/micro/10.1099/00221287-132-1-91&mimeType=html&fmt=ahah

References

  1. Andersen K., Wilke-Douglas M. 1984; Construction and use of a gene bank of Alcaligenes eutrophus in the analysis of ribulose bisphosphate carboxylase genes. Journal of Bacteriology 159:973–978
    [Google Scholar]
  2. Andersen K., Tait R. C., King W. R. 1981; Plasmids required for utilization of molecular hydrogen by Alcaligenes eutrophus. Archives of Microbiology 129:384–390
    [Google Scholar]
  3. Bowien B., Schlegel H. G. 1981; Physiology and biochemistry of aerobic hydrogen-oxidizing bacteria. Annual Review of Microbiology 35:405–452
    [Google Scholar]
  4. Bowien B., Friedrich B., Friedrich C. G. 1984; Involvement of megaplasmids in heterotrophic derepression of the carbon dioxide assimilating enzyme system in Alcaligenes spp. Archives of Microbiology 139:305–310
    [Google Scholar]
  5. Brewin N. J., DeJong T. M., Phillips D. A., Johnston A. W. B. 1980; Co-transfer of determinants for hydrogenase activity and nodulation ability in Rhizobium leguminosarum. Nature, London 288:77–79
    [Google Scholar]
  6. Cammack R., Hall D. O., Rao KiK. 1984; Hydrogenases: structure and applications in hydrogen production. pp. Edited by R. K. Poole & C. Dow. New York; Academic Press. In Microbial Gas Metabolism: Mechanistic, Metabolic and Biotechnological Aspects75–102
    [Google Scholar]
  7. Chippaux M., Giudici D., Abou-Jaoude A., Casse F., Pascal M. C. 1978; A mutation leading to the total lack of nitrite reductase activity in Escherichia coli KI2. Molecular and General Genetics 160:225–229
    [Google Scholar]
  8. Friedrich C. G. 1982; Derepression of hydrogenase during limitation of electron donors arid derepression of ribulose-bisphosphate carboxylase during carbon limitation of Alcaligenes eutrophus. Journal of Bacteriology 149:203–210
    [Google Scholar]
  9. Friedrich B., Hogrefe C., Schlegel H. G. 1981a; Naturally occurring genetic transfer of hydrogen-oxidizing ability between strains of Alcaligenes eutrophus. Journal of Bacteriology 147:198–205
    [Google Scholar]
  10. Friedrich C. G., Friedrich B., Bowien B. 1981b; Formation of enzymes of autotrophic metabolism during heterotrophic growth of Alcali-genes eutrophus. Journal of General Microbiology 122:69–78
    [Google Scholar]
  11. Gerstenberg C., Friedrich B., Schlegel H. G. 1982; Physical evidence for plasmids in autotrophic, especially hydrogen-oxidizing bacteria. Archives of Microbiology 133:90–96
    [Google Scholar]
  12. Haugland R. A., Cantrell M. A., Beaty J. S., Hanus F. J., Russel S. A., Evans H. J. 1984; Characterization of Rhizobium japonicum hydrogen uptake genes. Journal of Bacteriology 159:1006–1012
    [Google Scholar]
  13. Hogrefe C., Friedrich B. 1984; Isolation and characterization of megaplasmid DNA from litho-autotrophic bacteria. Plasmid 12:161–169
    [Google Scholar]
  14. Hogrefe C., Romermann D., Friedrich B. 1984; Alcaligenes eutrophus hydrogenase genes (Hox). Journal of Bacteriology 158:43–48
    [Google Scholar]
  15. Kado C. J., Liu S. T. 1981; Rapid procedure for detection and isolation of large and small plasmids. Journal of Bacteriology 145:1365–1373
    [Google Scholar]
  16. Krasna A. I. 1984; Mutants of Escherichia coli with altered hydrogenase activity. Journal of General Microbiology 130:779–787
    [Google Scholar]
  17. Leadbeater L., Siebert K., Schobert P., Bowien B. 1982; Relationships between activities and protein levels of ribulose-bisphosphate carboxylase and phosphoribulokinase in Alcaligenes eutrophus. FEMS Microbiology Letters 14:263–266
    [Google Scholar]
  18. Pootjes C. F. 1977; Evidence for plasmid coding of the ability to utilize hydrogen gas by Pseudomonas facilis. Biochemical and Biophysical Research Communications 76:1002–1006
    [Google Scholar]
  19. Schink B., Schlegel H. G. 1978; Mutants of Alcaligenes eutrophus defective in autotrophic metabolism. Archives of Microbiology 117:123–129
    [Google Scholar]
  20. Schink B., Schlegel H. G. 1979; The membranebound hydrogenase of Alcaligenes eutrophus. Biochimica et biophysica acta 567:315–324
    [Google Scholar]
  21. Schink B., Schlegel H. G. 1980; The membranebound hydrogenase of Alcaligenes eutrophus. II. Localization and immunological comparison to other hydrogenase systems. Antonie van Leeuwenhoek 46:1–14
    [Google Scholar]
  22. Schlegel H. G., Kaltwasser H., Gottschalk G. 1961; Ein Submersverfahren zur Kultur wasser-stoffoxidierender Bakterien; wachstumsphysio-logische Untersuchungen. Archiv fur Mikrobiologie 38:209–222
    [Google Scholar]
  23. Schlegel H. G., Meyer M. 1985; Isolation of hydrogenase regulatory mutants of hydrogen-oxidizing bacteria by a colony screening method. Archives of Microbiology 141:377–383
    [Google Scholar]
  24. Schneider K., Schlegel H. G. 1977; Localisation and stability of hydrogenases from aerobic hydrogen bacteria. Archives of Microbiology 112:229–238
    [Google Scholar]
  25. Simon R., Priefer U., Puhler A. 1983; Vector plasmids for in-vivo and in-vitro manipulations of Gram-negative bacteria. Edited by A. Puhler. Berlin: Springer-Verlag. . In Molecular Genetics of the Bacteria-Plant Interaction98–106
    [Google Scholar]
  26. Srivastava S., Urban M., Friedrich B. 1982; Mutagenesis of Alcaligenes eutrophus by insertion of the drug-resistance transposon Tn5. Archives of Microbiology 131:203–207
    [Google Scholar]
  27. Yates M. G., Robson R. L. 1985; Mutants of Azotobacter chroococcum defective in hydrogenase activity. Journal of General Microbiology 131:1459–1466
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-132-1-91
Loading
/content/journal/micro/10.1099/00221287-132-1-91
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error