1887

Abstract

SUMMARY: Deletion mutants of recombinant plasmids encoding the KS71B fimbrial antigens of the uropathogenic strain KS71 (O4:K12) were constructed. The effects of these mutations were tested by transforming the mutated plasmids into non-fimbriated HB101 cells and testing the transformants for fimbriation and haemagglutination. A deletion transcriptionally upstream from the fimbrial subunit gene increased the expression of KS71B fimbriae. Deletion of the fimbrial subunit gene resulted in non-fimbriated but haemagglutinating transformants, whereas a deletion 6 kb transcriptionally downstream from the subunit gene resulted in non-haemagglutinating but fimbriate transformants, indicating that fimbriation and haemagglutination were genetically separable. We also present evidence suggesting that the fimbrillin and haemagglutinin are physically associated in the wild-type KS71 strain.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-132-1-71
1986-01-01
2021-08-02
Loading full text...

Full text loading...

/deliver/fulltext/micro/132/1/mic-132-1-71.html?itemId=/content/journal/micro/10.1099/00221287-132-1-71&mimeType=html&fmt=ahah

References

  1. Altenbuchner J., Schmid K., Scmitt R. 1983; Tn 1721 -encoded tetracycline resistance: mapping of structural and regulatory genes mediating resistance. Journal of Bacteriology 153:116–123
    [Google Scholar]
  2. Båga M., Normark S., Hardy J., O’Hanley P., Lark D., Olsson O., Schoolnik G., Falkow S. 1984; Nucleotide sequence of the papA gene encoding the pap pilus subunit of human uropatho-genic Escherichia coli. Journal of Bacteriology 157:330–333
    [Google Scholar]
  3. Birnboim H. C., Doly J. 1979; A rapid alkaline extraction procedure for screening recombinant plasmid DNA. Nucleic Acids Research 7:1513–1517
    [Google Scholar]
  4. Bolivar F., Rodriguez R. L., Greene P. J., Betlach M. C., Heynecker H. L., Boyer H. W., Crosa J. H., Falkow S. 1977; Construction and characterization of new cloning vehicles. II. A multipurpose cloning system. Gene 2:95–113
    [Google Scholar]
  5. Brinton C. C. 1965; The structure, function, synthesis, and genetic control of bacterial pili and a model for DNA and RNA transport in Gramnegative bacteria. Transactions of the New York Academy of Sciences 27:1003–1054
    [Google Scholar]
  6. Chang A. C., Cohen S. N. 1978; Construction and characterization of amplifiable multicopy DNA cloning vehicles derived from the P15A cryptic miniplasmid. Journal of Bacteriology 134:1141–1156
    [Google Scholar]
  7. Clegg S., Pierce J. K. 1983; Organization of genes responsible for the production of mannoseresistant fimbriae of a uropathogenic Escherichia coli isolate. Infection and Immunity 42:900–906
    [Google Scholar]
  8. Duguid J. P., Smith I. W., Dempster G., Edmunds P. N. 1955; Non-flagellar filamentous appendages (‘fimbriae’) and haemagglutination activity in Bacterium coli. Journal of Pathology and Bacteriology 70:335–348
    [Google Scholar]
  9. Gaastra W., de Graaf F. K. 1982; Host-specific fimbrial adhesins of non-invasive enterotoxigenic Escherichia coli strains. Microbiological Reviews 46:129–161
    [Google Scholar]
  10. de Graaf F. K., Krenn B. E., Klaasen P. 1984; Organization and expression of genes involved in the biosynthesis of K99 fimbriae. Infection and Immunity 43:508–514
    [Google Scholar]
  11. Korhonen T. K., Eden S., Svanborg Eden C. 1980a; Binding of purified Escherichia coli pili to human urinary tract epithelial cells. FEMS Microbiology Letters 7:237–240
    [Google Scholar]
  12. Korhonen T. K., Nurmiaho E.-L., Ranta H., Svanborg Eden C. 1980b; New method for isolation of immunologically pure pili from Escherichia coli. Infection and Immunity 27:569–575
    [Google Scholar]
  13. Korhonen T. K., Leffler H., Svanborg Eden C. 1981; Binding specificity of piliated strains of Escherichia coli and Salmonella typhimurium to epithelial cells, Saccharomyces cerevisiae cells, and erythrocytes. Infection and Immunity 32:769–804
    [Google Scholar]
  14. Korhonen T. K., Väisänen V., Saxen H., Hult-BERG H., Svenson S. B. 1982; P-antigen-recognizing fimbriae from human uropathogenic Escherichia coli strains. Infection and Immunity 37:286–291
    [Google Scholar]
  15. Korhonen T. K., Rhen M., Väisänen-Rhen V., Pere A. 1985a; Antigenic and functional properties of enterobacterial fimbriae. pp. Edited by D. E. S. Stewart-Tull & M. Davies. Chichester: John Wiley & Sons. In Immunology of the Bacterial Cell Wall319–353
    [Google Scholar]
  16. Korhonen T. K., Valtonen V. M., Parkkinen J., Väisänen-Rhen V., Finne J., ørskov F., ørskov L., Svenson S. B., Makela P. H. 1985b; Serotypes, hemolysine production, and receptor recognition of Escherichia coli strains associated with neonatal sepsis and meningitis. Infection and Immunity 48:486–491
    [Google Scholar]
  17. Lindberg F. P., Lund B., Normark S. 1984; Genes of pyelonephritogenic Escherichia coli required for digalactoside specific agglutination of human cells. EMBO Journal 3:1167–1173
    [Google Scholar]
  18. Mandel M., Higa A. 1970; Calcium dependent bacteriophage DNA infection. Journal of Molecular Biology 53:154–162
    [Google Scholar]
  19. Maniatis T., Fritsch E. F., Sambrook J. 1982; Molecular Cloning: A Laboratory Manual. Cold Spring Harbor: Cold Spring Harbor Laboratory.
    [Google Scholar]
  20. Messinger J., Vieira J. 1982; A new pair of M13 vectors for selecting either DNA strand of doubledigest restriction fragments. Gene 19:269–276
    [Google Scholar]
  21. Mooi F. R., Harms N., Bakker D., de Graaf F. K. 1981; Organization and expression of genes involved in the production of the K88ab antigen. Infection and Immunity 32:1155–1163
    [Google Scholar]
  22. Norgren M., Normark S., Lark D., O’Hanley P., Schoolnik G., Falkow S., Svanborg Eden C., BAga M., Uhlin B. E. 1984; Mutations in E. coli cistrons affecting adhesion to human cells do not abolish Pap pili fiber formation. EMBO Journal 3:1159–1165
    [Google Scholar]
  23. Normark S., Lark D., Hull R., Norgren M., BAga M., O’Hanley P., Schoolnik G., Falkow S. 1983; Genetics of a digalactoside binding adhesin from a uropathogenic Escherichia coli strain. Infection and Immunity 41:942–948
    [Google Scholar]
  24. Nowicki B., Rhen M., Väisänen-Rhen V., Pere A., Korhonen T. K. 1984; Immunofluorescence study of fimbrial phase variation in Escherichia coli KS71. Journal of Bacteriology 160:691–695
    [Google Scholar]
  25. Nowicki B., Rhen M., Väisänen-Rhen V., Pere A., Korhonen T. K. 1985; Fractionation of a bacterial cell population by absorption to erythrocytes and yeast cells. FEMS Microbiology Letters 26:35–40
    [Google Scholar]
  26. ørskov L., ørskov F., Birch-Andersen A. 1980; Comparison of Escherichia coli fimbrial antigen F7 with type-1 fimbriae. Infection and Immunity 27:657–666
    [Google Scholar]
  27. ørskov L., ørskov F., Birch-Andersen A., Kana-mori M., Svanborg Eden C. 1982; O, K, H, and fimbrial antigens in Escherichia coli associated with pyelonephritis and cystitis. Scandinavian Journal of Infectious Diseases, Suppiementum 33:18–25
    [Google Scholar]
  28. Porter R. R. 1959; The hydrolysis of rabbit gammaglobulin and antibodies with crystalline papain. Biochemical Journal 13:119–126
    [Google Scholar]
  29. Rhen M. 1985; Characterization of DNA fragments encoding fimbriae of the uropathogenic Escherichia coli strain KS71. Journal of General Microbiology 131:571–580
    [Google Scholar]
  30. Rhen M., Klemm P., Wahlstrom E., Svenson S. B., Kallenius G., Korhonen T. K. 1983a; P-fimbriae of Escherichih coli: immuno- and protein-chemical characterization of fimbriae from two pyelonephritogenic strains. FEMS Microbiology Letters 18:233–238
    [Google Scholar]
  31. Rhen M., Knowles J., Penttila M. E., Sarvas M., Korhonen T. K. 1983b; P-fimbriae of Escherichia coli. molecular cloning of DNA fragments containing the structural genes. FEMS Microbiology Letters 19:119–123
    [Google Scholar]
  32. Rhen M., Makela P. H., Korhonen T. K. 1983c; P-fimbriae of Escherichia coli are subjected to phase variation. FEMS Microbiology Letters 19:267–271
    [Google Scholar]
  33. Rhen M., Väisänen-Rhen V., Pere A., Korhonen T. K. 1985a; Complementation and regulatory interaction between two cloned fimbrial gene clusters of Escherichia coli strain KS71. Molecular and General Genetics 200:60–64
    [Google Scholar]
  34. Rhen M., Van Die L., Väisänen-Rhen V., Bergmans H. 1985b; The nucleotide sequences of the genes encoding the KS71A and F7j fimbrial subunits of uropathogenic Escherichia coli. European Journal of Biochemistry 151:573–577
    [Google Scholar]
  35. Roberts J. A., Hardaway K., Kaack B., Fussel E. N., Baskin G. 1984; Prevention of pyelonephritis by immunization with P-fimbriae. Journal of Urology 131:602–607
    [Google Scholar]
  36. Sanger F., Nicklen S., Coulson A. R. 1977; DNA sequencing with chain terminating inhibitors. Proceedings of the National Academy of Sciences of the United States of America 74:5463–5467
    [Google Scholar]
  37. Svanborg Eden C., Hansson H. A. 1978; Escherichia coli pili as possible mediators of attachment to human urinary tract epithelial cells. Infection and Immunity 21:229–237
    [Google Scholar]
  38. Väisänen V., Elo J., Tallgren L. G., Siitonen A., Makela P. H., Svanborg Eden C., Kallenius G., Svenson S. B., Hultberg H., Korhonen T. K. 1981; Mannose-resistant haemagglutination and P antigen recognition are characteristic of Escherichia coli causing primary pyelonephritis. Lancet ii:1366–1369
    [Google Scholar]
  39. Väisänen-Rhen V., Elo J., Väisänen E., Siitonen A., ørskov L., ørskov F., Svenson S. B., Makela P. H., Korhonen T. K. 1984; P-fimbriated clones among uropathogenic Escherichia coli strains. Infection and Immunity 43:149–155
    [Google Scholar]
  40. Van Die I., Bergmans H. 1984; Nucleotide sequence of the gene encoding the F72 fimbrial subunit of an uropathogenic Escherichia coli strain. Gene 32:83–90
    [Google Scholar]
  41. Van Die L., Van Megen L., Hoekstra W., Bergmans H. 1984; Molecular organization of the genes involved in the production of F72 fimbriae, causing mannose resistant haemagglutination of a uropathogenic Escherichia coli 06: K2 : H1 : F7 strain. Molecular and General Genetics 194:528–533
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-132-1-71
Loading
/content/journal/micro/10.1099/00221287-132-1-71
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error